The F1-type ATPase in anaerobic Lactobacillus casei

Muntyan, Maria S.; Mesyanzhinova, Irina V.; Milgrom, Yakov M.; Skulachev, Vladimir P.

doi:10.1016/0005-2728(90)90171-y

Cited by 7 publications

(2 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, the levels of inhibition of MB F 1Ec (or F 1 F oEc ) at the above concentrations of DCCD and Na azide were between 92 and 98%. A higher tolerance of MB and soluble F 1 F oCp or F 1Cp to inhibition by DCCD and Na azide is not unusual, as similar results were reported for several gram-positive bacteria (3,26,28,32,49). A comparison of the ATPase activities of DM-and EDTA-extracted membranes with that of untreated membranes indicated that about 87 and 70% of ATPase activities were solubilized from membranes by DM and EDTA, respectively ( Table 2).…”

Section: I) Atpb(a) Atpe(c) Atpf(b) Atph(␦) Atpa(␣) Atpg(␥) Atsupporting

confidence: 80%

Clostridium pasteurianum F ₁ F _o ATP Synthase: Operon, Composition, and Some Properties

Das

Ljungdahl

2003

J Bacteriol

View full text Add to dashboard Cite

Section: I) Atpb(a) Atpe(c) Atpf(b) Atph(␦) Atpa(␣) Atpg(␥) Atsupporting

confidence: 80%

Clostridium pasteurianum F ₁ F _o ATP Synthase: Operon, Composition, and Some Properties

Das

Ljungdahl

2003

J Bacteriol

View full text Add to dashboard Cite

“…A number of attempts have been made to determine relative molar subunit stoichiometries of multi-subunit protein complexes from scanned electrophoresis profiles after protein staining with Coomassie Brilliant Blue dye [20][21][22][23][24][25][26][27][28][29]. The quantitative reliability of such measurements is questionable, however, especially considering that staining intensity for this dye is only quantitatively related to protein mass when comparing identical or very similar proteins.…”

Section: Discussionmentioning

confidence: 99%

Comparison of three different fluorescent visualization strategies for detectingEscherichia coli ATP synthase subunits after sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Berggren¹,

Chernokalskaya

Lopez³

et al. 2001

Proteomics

View full text Add to dashboard Cite

The correlation between protein molecular weight and the number of lysine or basic amino acid residues was found to be high for broad range molecular weight standards, subunits of Escherichia coli F1F0-ATP synthase and the translated open reading frame of E. coli. A relatively poor correlation between protein molecular weight and the number of cysteine residues was observed in all cases. The ability of amine-reactive, thiol-reactive and basic amino acid-binding fluorophores to detect the eight subunits of F1F0-ATP synthase complex was assessed using 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF), monobromobimane (MBB) and SYPRO Ruby protein gel stain, respectively. Though experimentally none of the fluorophores provided accurate estimates of the subunit stoichiometry of this complex, MDPF and SYPRO Ruby protein gel stain were capable of semiquantitative detection of every subunit. MBB, however, failed to detect subunits a, b and c of the hydrophobic F0 complex, as well as subunit epsilon of the F1 complex. All three fluorescent detection procedures permitted subsequent identification of representative subunits by peptide mass profiling using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The use of thiol-reactive fluorophores for the global analysis of protein expression profiles does not appear to be advisable as a significant number of proteins have few or no cysteine residues, thus escaping detection.

show abstract

Chemiosmotic systems in bioenergetics: H+-cycles and Na+-cycles

Skulachev

1991

Bioscience Reports

View full text Add to dashboard Cite

The development of membrane bioenergetic studies during the last 25 years has clearly demonstrated the validity of the Mitchellian chemiosmotic H+ cycle concept. The circulation of H+ ions was shown to couple respiration-dependent or light-dependent energy-releasing reactions to ATP formation and performance of other types of membrane-linked work in mitochondria, chloroplasts, some bacteria, tonoplasts, secretory granules and plant and fungal outer cell membranes. A concrete version of the direct chemiosmotic mechanism, in which H+ potential formation is a simple consequence of the chemistry of the energy-releasing reaction, is already proved for the photosynthetic reaction centre complexes. Recent progress in the studies on chemiosmotic systems has made it possible to extend the coupling-ion principle to an ion other than H+. It was found that, in certain bacteria, as well as in the outer membrane of the animal cell, Na+ effectively substitutes for H+ as the coupling ion (the chemiosmotic Na+ cycle). A precedent is set when the Na+ cycle appears to be the only mechanism of energy production in the bacterial cell. In the more typical case, however, the H+ and Na+ cycles coexist in one and the same membrane (bacteria) or in two different membranes of one and the same cell (animals). The sets of delta mu H+ and delta mu Na+ generators as well as delta mu H+ and delta mu Na+ consumers found in different types of biomembranes, are listed and discussed.

show abstract

The F1-type ATPase in anaerobic Lactobacillus casei

Cited by 7 publications

References 44 publications

Clostridium pasteurianum F ₁ F _o ATP Synthase: Operon, Composition, and Some Properties

Clostridium pasteurianum F ₁ F _o ATP Synthase: Operon, Composition, and Some Properties

Comparison of three different fluorescent visualization strategies for detectingEscherichia coli ATP synthase subunits after sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Chemiosmotic systems in bioenergetics: H+-cycles and Na+-cycles

Contact Info

Product

Resources

About

The F1-type ATPase in anaerobic Lactobacillus casei

Cited by 7 publications

References 44 publications

Clostridium pasteurianum F 1 F o ATP Synthase: Operon, Composition, and Some Properties

Clostridium pasteurianum F 1 F o ATP Synthase: Operon, Composition, and Some Properties

Comparison of three different fluorescent visualization strategies for detectingEscherichia coli ATP synthase subunits after sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Chemiosmotic systems in bioenergetics: H+-cycles and Na+-cycles

Contact Info

Product

Resources

About

Clostridium pasteurianum F ₁ F _o ATP Synthase: Operon, Composition, and Some Properties

Clostridium pasteurianum F ₁ F _o ATP Synthase: Operon, Composition, and Some Properties