The Fate of Hydrocortisone-4-14c in the Tissues of the Mouse

Abstract

When hydrocortisone-4-14C is injected intravenously into mice, the whole of the 14C remains in a form which can be recovered from the carcase by extraction with 70% aqueous methanol.The fraction of recovered 14C which remains soluble in chloroform declines rapidly at first and more slowly thereafter, so that at 1 hr less than 15 % is still chloroform soluble.Glucuronidase increases the chloroform soluble fraction slightly, and a further small amount is made chloroform soluble by acid hydrolysis. The latter fraction seems to increase with time.The rate of destruction of true hydrocortisone has been measured. There is a rapid decomposi-

Increased Corticosteroid Binding ("Transcortin") Activity in Adrenalectomized and Hypophysectomized Rats

“…Similarly, the passage of cortisol into the put of adrenalectomized mice is slower than in normals (3). It was of interest to investigate whether this ~irolongcd retention of cxticosteroid hormoc in the ldood of adrenalectomized animal&, might brt causally related to quantitatiw changes in the activity of the specific corticosteroid-tinding protein system, "transcortin" (for references, see ( 4 ) ) .…”