The fate of recombinant plant DNA in soil

Paget, Eric; Lebrun, Michel; Freyssinet, Georges; Simonet, Pascal

doi:10.1016/s1164-5563(99)90005-5

Cited by 87 publications

(57 citation statements)

References 41 publications

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“…2) suggests that the number of aadA sequences quantified by real-time PCR is only a relative indicator of the biological potential. These data also confirm the need for additional studies to determine if persistent transgenic sequences detected in field soils (12,20) maintain their biological potential.…”

supporting

confidence: 64%

“…When experiments were conducted with soil microcosms, PCR tests indicated that most of the plant DNA was rapidly lost, although a proportion remained detectable in the soil (28,29). Although others have found detectable transgenic plant sequences in field soil, where the presence of plant tissues was no longer detectable (12,20), the decaying plant process certainly affects DNA stability and persistence over time (27). During plant senescence, active nucleases can degrade DNA.…”

mentioning

confidence: 99%

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Degradation and Transformability of DNA from Transgenic Leaves

Ceccherini

Poté

Kay

et al. 2003

Appl Environ Microbiol

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The fate of transplastomic (chloroplast genome contains the transgene) tobacco plant DNA in planta was studied when the plant leaves were subjected to decay conditions simulating those encountered naturally, including grinding, incubation with cellulase or enzymes produced by Erwinia chrysanthemi, and attack by the plant pathogen Ralstonia solanacearum. Direct visualization of DNA on agarose gels, gene extraction yield (the number of amplifiable aadA sequences in extracted plant DNA), and the frequency that recipient bacteria can be transformed by plant DNA were used to evaluate the quality and quantity of plant DNA and the transgene. These measurements were used to monitor the physical and biological degradation of DNA inside decaying plant tissues. Our results indicate that while most of the DNA will be degraded inside plant cells, sufficient DNA persists to be released into the soil.

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supporting

confidence: 64%

mentioning

confidence: 99%

Degradation and Transformability of DNA from Transgenic Leaves

Ceccherini

Poté

Kay

et al. 2003

Appl Environ Microbiol

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“…All the above data and available evidence point to the same conclusion: horizontal gene transfer from transplastomic plants to bacteria might happen but only under very specific, optimized conditions, e.g., in the presence of homologous sequences and highly competent bacteria, and at an extremely low frequency, i.e., around or just above the detection limit of the actually applied methods. This conclusion is supported by ample evidence that under field conditions, though with nuclear transgenic plants, no horizontal gene transfer has been detected so far (Paget et al 1998;Gebhard and Smalla 1999;Badosa et al 2004;Demanèche et al 2008;Wagner et al 2008;Kim et al 2010;Isaza et al 2011;Ma et al 2011).…”

Section: −8mentioning

confidence: 84%

Transplastomic plants for innovations in agriculture. A review

Wani

Sah

Sági

et al. 2015

Agron. Sustain. Dev.

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Food production has to be significantly increased in order to feed the fast growing global population estimated to be 9.1 billion by 2050. The Green Revolution and the development of advanced plant breeding tools have led to a significant increase in agricultural production since the 1960s. However, hundreds of millions of humans are still undernourished, while the area of total arable land is close to its maximum utilization and may even decrease due to climate change, urbanization, and pollution. All these issues necessitate a second Green Revolution, in which biotechnological engineering of economically and nutritionally important traits should be critically and carefully considered. Since the early 1990s, possible applications of plastid transformation in higher plants have been constantly developed. These represent viable alternatives to existing nuclear transgenic technologies, especially due to the better transgene containment of transplastomic plants. Here, we present an overview of plastid engineering techniques and their applications to improve crop quality and productivity under adverse growth conditions. These applications include (1) transplastomic plants producing insecticidal, antibacterial, and antifungal compounds. These plants are therefore resistant to pests and require less pesticides. (2) Transplastomic plants resistant to cold, drought, salt, chemical, and oxidative stress. Some pollution tolerant plants could even be used for phytoremediation. (3) Transplastomic plants having higher productivity as a result of improved photosynthesis. (4) Transplastomic plants with enhanced mineral, micronutrient, and macronutrient contents. We also evaluate field trials, biosafety issues, and public concerns on transplastomic plants. Nevertheless, the transplastomic technology is still unavailable for most staple crops, including cereals. Transplastomic plants have not been commercialized so far, but if this crop limitation were overcome, they could contribute to sustainable development in agriculture.

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“…Plusieurs études ont été menées pour évaluer l'incidence des transferts horizontaux impliquant des molécules d'ADN recombinant dans le milieu édaphique (Bertolla et Simonet 1999;Gebhard et Smalla 1998, 1999Nielsen et al 1998;Paget et al 1998;Schütler et al 1995;Widmer et al 1997). En bref, aucune de ces études n'a permis de démontrer l'incidence d'évènements de transformation géné-tique au sein des populations bactériennes isolées du sol, malgré la présence d'ADN libre à proximité.…”

Section: L'incidence Des Transferts (Trans)géniques Horizontauxunclassified

Impact environnemental des cultures transgéniques

Michaud

2006

phyto

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L’adoption à grande échelle des cultures transgéniques depuis dix ans a soulevé de nombreuses questions quant aux impacts possibles de ces nouvelles lignées végétales sur les écosystèmes agricoles et naturels. Des questions ont été soulevées, en particulier, sur le devenir des transgènes dans le milieu et sur une possible « pollution » du patrimoine génétique des organismes vivants à l’échelle des écosystèmes. Après une énumération des impacts environnementaux associés aux végétaux transgéniques, cet article de synthèse dresse un aperçu des connaissances actuelles sur le devenir – ou la migration – des transgènes dans le milieu. Les phénomènes d’hybridation et d’introgression génique en direction d’espèces ou de lignées apparentées sont d’abord abordés, après quoi sont considérés les phénomènes de transfert horizontal des transgènes en direction d’organismes non apparentés. Un article complémentaire publié dans ce même numéro traite de l’impact environnemental des protéines recombinantes encodées par les transgènes (Michaud 2005).

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The fate of recombinant plant DNA in soil

Cited by 87 publications

References 41 publications

Degradation and Transformability of DNA from Transgenic Leaves

Degradation and Transformability of DNA from Transgenic Leaves

Transplastomic plants for innovations in agriculture. A review

Impact environnemental des cultures transgéniques

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