The fatty acid synthase gene in avian liver. Two mRNAs are expressed and regulated in parallel by feeding, primarily at the level of transcription.

Characterization of a genomic and cDNA clone coding for the thioesterase domain and 3' noncoding region of the chicken liver fatty acid synthase gene

“…Downstream of the consensus polyadenylation signal sequence AATAAA, the 3' end formation signal sequences "CA and G/T cluster" starting at 3377 bp (Lai et al, 1979;McLauchlan et al, 1985) are also found (Figure 5). Back et al (1986) have found that chicken liver contains two mRNAs for fatty acid synthase, using goose cDNA as a probe. Our S[ mapping studies have indicated that chicken FAS mRNA is a single species, a conclusion that is further supported by the presence of only one potential poly(A+) addition signal sequence.…”

“…The changes in the synthase activity and content under these various conditions are due in part to alterations in the rate of enzyme synthesis (Zehner et al, 1977; Fischer & Goodridge, 1978;Joshi & Aranda, 1979;Morris et al, 1982;Goodridge et al, 1984;Nepokroeff et al, 1984). Such changes are accompanied or preceded by corresponding changes in the levels of fatty acid synthase mRNA (Morris et al, 1982(Morris et al, , 1984Goodridge et al, 1984) suggesting that the regulation of synthase mRNA is predominantly at the level of gene transcription (Back et al, 1986). However, the precise molecular mechanism by which the changes in synthase protein are regulated is not yet known.…”

“…The 1 Abbreviations: FAS, fatty acid synthase; bp, base pair(s); kb, kilobase(s); kbp, kilobase pair(s); SDS, sodium dodecyl sulfate; TE1 and TE2, oligonucleotide probes; TE, thioesterase. 0006-2960/88/0427-7778S01.50/0 © 1988 American Chemical Society synthase mRNAs of goose uropygial gland and liver (Zehner et al, 1980;Morris et al, 1982;Back et al, 1986) and lactating rat mammary gland (Mattick et al, 1981) are estimated to be about 10-16 kb, which is about twice the number of nucleotides required for the 2300 amino acids needed for the subunit protein synthesis. The excess nucleotides present in the mRNA would have to be distributed between the 5' and 3' untranslated regions of the molecule.…”

Expression patterns of AMPK and genes associated with lipid metabolism in newly hatched chicks during the metabolic perturbation of fasting and refeeding

“…FAS is a key enzyme in fatty acid synthesis. In the chick liver, FAS activity and expression level are related to the rate of fatty acid synthesis, and the change in FAS mRNA expression is positively correlated with the rate of FAS protein synthesis ( Back et al, 1986 ). In this experiment, the FAS expression in liver tissues of Group C was increased ( P < 0.05) at F24 and F48.…”

The Simvastatin Effect on Acetyl Coa Carboxylase1 and Fatty Acid Synthase Gene Expression in Rat Fed With High Fat Diets and Its Association With Fatty Acid Proportion and Concentration

“…The other studies have demonstrated that the relative mRNA level and the rate of synthesis of FAS increase in mouse pre -adipocytes 42 and upon feeding in the avian liver 41 which was in agreement with our study that the FAS mRNA expression was increased when all groups compared to the G1, but it was not significant (P> 0.05). Study of Back et al 43 have indicated that feeding and fasting regulated FAS synthesis at the level of transcription. Slight increase in FAS mRNA may be the reason of a slight increase in some fatty acid consumptions.…”