The feeding status regulates the action of insulin and 20-hydroxyecdysone on haemolymph trehalose level in Bombyx larvae

Kh., Sanathoibi D.; Keshan, Bela

doi:10.1016/j.cbpb.2021.110579

Cited by 6 publications

(5 citation statements)

References 38 publications

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“…Trehalase gene expression was found to be regulated by the ILP signaling pathway, as demonstrated experimentally in a variety of insects. In Bombyx mori larvae, trehalase‐2 mRNA expression increased after insulin treatment of food‐deprived larvae 57 . Furthermore, in Tribolium castaneum , the expression of genes encoding trehalase was regulated by the ILP2 and IIS pathways 58 .…”

Section: Discussionmentioning

confidence: 99%

“…In Bombyx mori larvae, trehalase-2 mRNA expression increased after insulin treatment of food-deprived larvae. 57 Furthermore, in Tribolium castaneum, the expression of genes encoding trehalase was regulated by the ILP2 and IIS pathways. 58 In the current study, the expression of the BtTre1 and BtTre2 genes was upregulated by ILP7 in B. tabaci MED.…”

Section: Discussionmentioning

confidence: 99%

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Effects of insulin‐like peptide 7 in Bemisia tabaciMED on tomato chlorosis virus transmission

Zhang

et al. 2023

Pest Management Science

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BACKGROUND Tomato chlorosis virus (ToCV) is a semi‐persistent plant virus that is primarily transmitted by the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae). It causes a serious disease that lowers tomato yield. Insulin‐like peptide (ILP), an insulin homolog, regulates trehalose metabolism in a variety of insects. In a previous study, we discovered that trehalose metabolism is required for whiteflies to transmit ToCV effectively. Furthermore, transcriptome sequencing revealed that the BtILP7 gene was highly expressed in B. tabaci infected with ToCV. Therefore, the whitefly ILP7 gene may facilitate the transmission of ToCV and be an attractive target for the control of whiteflies and subsequently ToCV. RESULTS The ToCV content in B. tabaci MED was found to be correlated with BtILP7 gene expression. Subsequent RNA interference (RNAi) of the BtILP7 gene had a significant impact on B. tabaci MED's trehalose metabolism and reproductive capacity, as well as ability to transmit ToCV. CONCLUSIONS These results indicate that the BtILP7 gene was closely related to ToCV transmission by regulating trehalose metabolism and reproduction behavior, thus providing a secure and environmentally friendly management strategy for the control of whiteflies and ToCV‐caused disease. © 2022 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of insulin‐like peptide 7 in Bemisia tabaciMED on tomato chlorosis virus transmission

Zhang

et al. 2023

Pest Management Science

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“…Several studies have demonstrated that 20E, an insect steroid hormone, plays a key role in regulating carbohydrate metabolism during the larval–pupal transition [ 20 ]. In Bombyx mori , 20E induces the accumulation of glycogen in the fat body of fed and food-deprived larvae and decreases the hemolymph trehalose levels by decreasing its synthesis in food-deprived larvae [ 8 ] but shows mild trehalose action in fed larvae [ 21 ]. In addition, the 20E treatment inhibited key glycolytic enzyme activity and mRNA levels in the fat body of B. mori larvae, whereas the knockdown of the 20E receptor EcR-USP had a contrary effect to the 20E treatment [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

20-Hydroxyecdysone and Receptor Interplay in the Regulation of Hemolymph Glucose Level in Honeybee (Apis mellifera) Larvae

Wang

Chen

et al. 2023

Metabolites

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The hormone 20-hydroxyecdysone (20E) and the ecdysone receptors (ECR and USP) play critical roles in the growth and metabolism of insects, including honeybees. In this study, we investigated the effect of 20E on the growth and development of honeybee larvae by rearing them in vitro and found reduced food consumption and small-sized pupae with increasing levels of 20E. A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based analysis of widely targeted metabolomics was used to examine the changes in the metabolites after an exogenous 20E application to honeybee larvae and the underlying mechanisms. A total of 374 different metabolites were detected between the control group and the 20E treatment group, covering 12 subclasses. The most significant changes occurred in 7-day-old larvae, where some monosaccharides, such as D-Glucose and UDP-galactose, were significantly upregulated. In addition, some metabolic pathways, such as glycolysis/gluconeogenesis and galactose metabolism, were affected by the 20E treatment, suggesting that the 20E treatment disrupts the metabolic homeostasis of honeybee larvae hemolymph and that the response of honeybee larvae to the 20E treatment is dynamic and contains many complex pathways. Many genes involved in carbohydrate metabolism, including genes of the glycolysis and glycogen synthesis pathways, were downregulated during molting and pupation after the 20E treatment. In contrast, the expression levels of the genes related to gluconeogenesis and glycogenolysis were significantly increased, which directly or indirectly upregulated glucose levels in the hemolymph, whereas RNA interference with the 20E receptor EcR-USP had an opposite effect to that of the 20E treatment. Taken together, 20E plays a critical role in the changes in carbohydrate metabolism during metamorphosis.

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“…Studies have found that the carbohydrate metabolism can be regulated by the 20-hydroxyecdysone (20E), which is an insect steroid hormone that involves in the regulation of molting (Colombani et al, 2005;Kh and Keshan, 2021). 20E is able to induce hunger by slowly reducing food consumption (Liu et al, 2010), and can inhibit the transcription of key glycolytic enzymes (Tian et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Inhibited expression of amylases and cellulases in crabs with hepatopancreatic necrosis disease and their regulation by EcR and HR38

Ren

Zhao

et al. 2023

Front. Mar. Sci.

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Hepatopancreatic necrosis disease (HPND) is a serious threat to the stable and sustainable development of the Chinese mitten crab farming industry. Studies have found that the hepatopancreas is the main lesion organ in HPND crabs, capable of causing metabolic abnormalities and non-feeding (starvation state). Here, four amylases (named as EsAmy1-4) and four cellulases (endo-beta-1, 4-glucanase, named as EsGlu1-4) that relate to carbohydrate metabolism were cloned from Eriocheir sinensis. Our studies found that the expressions of EsAmy1-4 and EsGlu1-4 were significantly decreased in the hepatopancreas of crabs with HPND. Moreover, the expressions of two important regulatory factors of carbohydrate metabolism, hormone receptor 38 (EsHR38) and ecdysone receptor (EsEcR), were remarkably decreased in the hepatopancreas of crabs with HPND. Further studies found that knockdown of EsHR38 and EsEcR could down-regulated the expressions of EsAmy1-4 and EsGlu1-4. In addition, downregulation of EsEcR could decrease the expression of EsHR38. Hence, HPND causes down-regulation of EsEcR expression, inhibits the transcription of EsHR38, further inhibits the expression of EsAmy1-4 and EsGlu1-4, and then leads to the abnormal carbohydrate metabolism. The abnormalities of carbohydrate metabolism may result in the abnormal food intake in crabs. This study provides new evidence for further understanding the mechanism of HPND.

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The feeding status regulates the action of insulin and 20-hydroxyecdysone on haemolymph trehalose level in Bombyx larvae

Cited by 6 publications

References 38 publications

Effects of insulin‐like peptide 7 in Bemisia tabaciMED on tomato chlorosis virus transmission

Effects of insulin‐like peptide 7 in Bemisia tabaciMED on tomato chlorosis virus transmission

20-Hydroxyecdysone and Receptor Interplay in the Regulation of Hemolymph Glucose Level in Honeybee (Apis mellifera) Larvae

Inhibited expression of amylases and cellulases in crabs with hepatopancreatic necrosis disease and their regulation by EcR and HR38

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