The first isoform-selective protein biosensor: a metallothionein potentiometric electrode

Capdevila, Mercè; González-Bellavista, Anna; Muñoz, Marı́a; Atrian, Sı́lvia; Fàbregas, Esteve

doi:10.1039/b922284g

Cited by 8 publications

(8 citation statements)

References 28 publications

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“…Hence voltammetry, chronopotentiometry and polarography applications have lowered the detection range to as low as 10 -7 to 10 -10 M, and recently even zeptomolar sensitivity has been reported. Despite these attractive perspectives, electrochemical approaches entail high preparation purity, due to the interference caused by different compounds in the sample [146]. Electrochemical methods are used mainly detection of MTs as environmental pollution biomarker.…”

Section: Electrochemical Methodsmentioning

confidence: 99%

“…In comparison to the bare carbon paste electrode the detection limit of the sensor was ten times lowered making it comparable to modified carbon paste electrodes. A precise, accurate and, significantly, selective electrodes for MT1 quantification, were presented by Capdevila et al [146] The membrane was created by embedding mammalian Zn-7-MT1 complexes as ionophores in a polysulfone matrix. It was proved that the presence of other mammalian MT isoforms do not interfere in MT1 measurement.…”

Section: Metallothionein As a Part Of Metal-detecting Electrochemicalmentioning

confidence: 99%

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Analytical Methods for Metallothionein Detection

Ryvolová¹,

Křížková²,

Adam³

et al. 2011

CAC

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Metallothioneins (MTs) are a group of low molecular mass, cysteine-rich proteins with a variety of functions including involvement in metal homeostasis, free radical scavenging, protection against heavy metal damage, and metabolic regulation via Zn donation. The overexpression of MTs in proliferating cells is turning the attention to the study of MTs as novel promising marker of tumor diseases. Besides, levels of MTs in invertebrates and aquatic vertebrates well correlate with heavy metal pollution of an environment and, thus, serve as bio-environmental marker. It is not surprising that these proteins are of great interest not only for biochemists, molecular biologists clinical chemists, but also for environmental chemists and ecologists.Detection and quantification of MTs, which is needed in all above mentioned areas, is challenging due to the high cysteine content and relatively low molecular mass. This review is giving an overview of the analytical methods used for determination of MTs comprising a broad range of immunochemical, electrochemical as well as spectrometric methods, which have been optimized and even hyphenated with different separation methods to detect MTs. More attention is paid to the structural analysis of these proteins as well as the employment of analytical instruments for environmental, biochemical and clinical purposes.

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Section: Electrochemical Methodsmentioning

confidence: 99%

Section: Metallothionein As a Part Of Metal-detecting Electrochemicalmentioning

confidence: 99%

Analytical Methods for Metallothionein Detection

Ryvolová¹,

Křížková²,

Adam³

et al. 2011

CAC

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“…The majority of known electrochemical biosensors are based on immobilized specific biomolecules, such as proteins, enzymes, nuclear acids, antibodies, and antigens on the modified electrodes [1][2][3][4][5][6][7][8]. These resulting biomolecule-based devices usually show high sensitivity and specificity due to the high loading of enzymes on the nanoparticles [9][10][11][12].…”

mentioning

confidence: 99%

Nanostructured Mimic Enzymes for Biocatalysis and Biosensing

Zhang

Wang

2011

NanoBiosensing

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Accurate, rapid, inexpensive, and selective analysis is required today for use in clinical diagnostics and the food industry. The majority of known electrochemical biosensors are based on immobilized specific biomolecules, such as proteins, enzymes, nuclear acids, antibodies, and antigens on the modified electrodes [1][2][3][4][5][6][7][8]. These resulting biomolecule-based devices usually show high sensitivity and specificity due to the high loading of enzymes on the nanoparticles [9][10][11][12]. However, their stability is limited due to easy denaturation [13] and leakage of biomolecules during their storage and immobilization procedure. Furthermore, the preparation and purification of biomolecules are usually time-consuming and expensive [14]. Therefore, the syntheses of artificial biomolecules with highly catalytic properties and a wide range of practical applications are becoming a significant field for different purposes.Artificial enzyme mimetics have attracted considerable interest because they can overcome the disadvantages of the natural enzyme [15][16][17][18]. Up to now, some coordination compounds have been prepared as artificial enzyme mimetics [19][20][21][22][23]. Through in vitro selection, nonnatural ribozymes, deoxyribozymes (catalytic DNA molecules) [24][25][26][27], and many other enzyme mimetics [28][29][30][31][32][33][34][35][36]

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“…Recently, Capevila et al demonstrated a biosensor membrane by embedding mammalian Zn 7 -MT1 complexes as ionophores in the analysis of MT1, which opens up a broad range of possibilities in the use of small proteins or metalloproteins as sensing elements for potentiometric sensors [7]. In this paper, we report a novel potentiometric detection strategy that makes use of protamine as sensing element in organic membrane to offer a very promising method for simple and selective sensing of heparin.…”

Section: Introductionmentioning

confidence: 99%

“…In many cases of practical relevance, however, blood electrolytes such as lipophilic anions thiocyanide (SCN À ) and salicylate (Sal À ) strongly interfere with the heparin response [4]. A significant advance in heparin detection involves the use of protamine sensor as endpoint detector titration of heparin with protamine to avoid such interferences, but such titration methodology complicates the analysis procedure [5,6].Recently, Capevila et al demonstrated a biosensor membrane by embedding mammalian Zn 7 -MT1 complexes as ionophores in the analysis of MT1, which opens up a broad range of possibilities in the use of small proteins or metalloproteins as sensing elements for potentiometric sensors [7]. In this paper, we report a novel potentiometric detection strategy that makes use of protamine as sensing element in organic membrane to offer a very promising method for simple and selective sensing of heparin.…”

mentioning

confidence: 99%