The first Japanese biobank of patient‐derived pediatric acute lymphoblastic leukemia xenograft models

Abstract

A lack of practical resources in Japan has limited preclinical discovery and testing of therapies for pediatric relapsed and refractory acute lymphoblastic leukemia (ALL), which has poor outcomes. Here, we established 57 patient‐derived xenografts (PDXs) in NOD.Cg‐Prkdcscidll2rgtm1Sug/ShiJic (NOG) mice and created a biobank by preserving PDX cells including three extramedullary relapsed ALL PDXs. We demonstrated that our PDX mice and PDX cells mimicked the biological features of relapsed ALL and that PDX models reproduced treatment‐mediated clonal selection. Our PDX biobank is a useful scientific resource for capturing drug sensitivity features of pediatric patients with ALL, providing an essential tool for the development of targeted therapies.

Patient-derived xenograft models in cancer therapy: technologies and applications

“… N/A Kamili et al 254 Neuroblastoma 9 (64%) Orthotopic inoculation Poor outcome Miyamoto et al 255 Cervical cancer (Adenocarcinoma, adenosquamous carcinoma, squamous cell carcinoma) 11 (50%) Large tumor size, high serum squamous cell carcinoma antigen and carbohydrate antigen 125 levels, and advanced FIGO stages. Clinically poor prognoses Schütte et al 173 Colorectal cancer (N/A) 59 (60%) N/A N/A Tanaka et al 256 Acute lymphoblastic leukemia (B- acute lymphoblastic leukemia, T-acute lymphoblastic leukemia) 57 (93.3%) N/A N/A Tew et al 257 Central nervous system metastasis (Adenocarcinoma, Squamous cell carcinoma, Invasive ductal carcinoma, etc.) 39 (84.8%) N/A N/A Chapuy et al 258 Diffuse large B-cell lymphoma (activated B-cell (ABC)-type tumors, germinal B-cell type tumors, and plasmablastic lymphoma) 9 (32%) N/A N/A Baschnagel et al 259 Small cell lung cancer brain metastases (Adenocarcinoma, sarcomatoid carcinoma) 9 (64%) N/A N/A Elst et al 260 Advanced penile cancer (Usual, warty-basaloid, sarcomatoid) 11 (61%) N/A N/A Lilja-Fischer et al 261 Oropharyngeal squamous cell carcinoma (N/A) 12 (35%) N/A N/A Zhang et al 262 B-cell lymphoma (Mantle cell lymphoma, Burkitt’s lymphoma, Follicular lymphoma, Marginal zone lymphoma, etc.)…”