The First Mitotic Cycle of Mesophyll Protoplasts

Meyer, Yves; Aspart, Lorette

doi:10.1007/978-3-0348-6776-4_9

Cited by 1 publication

(1 citation statement)

References 59 publications

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“…",/-fusion: protoplasts can be synchronized with or without blocking cell wall synthesis (30); use of Go cells may favour enhanced recombination exchanges during heterokaryon formation from Go to S phase; 'Egg transformation': pollen is synchronized in G2; produced subchromatid breaks are essential in terms of chromosome pairing, recombination, and repair (37).…”

Section: The Cell Cycle Stagementioning

confidence: 99%

Progress in cellular engineering of plants: biochemical and genetic assessment of selectable markers from cultured cells

1984

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Recent availability of stable and well characterized selectable markers and ability to combine alien genomes parasexually have contributed to the development of molecular biology in higher plants, including gene expression and genetic manipulation.Several types of biochemical mutants (resistant to inhibitory concentrations of aminoacid(s) or aminoacid analogs as well as deficient for enzyme activity) have recently been isolated and characterized biochemically and genetically. Among them, mutants with alterations in the nitrogen and aminoacid metabolism, or in the activity of alcohol dehydrogenases are being used in the development of more efficient techniques of gene transfer.The manipulation of whole genomes by sexual or somatic cell fusion offers new potential in this field, but refinement of transfer techniques is desirable. The new set of selectable markers obtained through advanced cellular technology, as well as our ability to regenerate plants from manipulated cell lines are expected to play a major role in cellular engineering.

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Section: The Cell Cycle Stagementioning

confidence: 99%