Palaeoecological and archaeological studies conducted in Mozgawa (southern Poland) demonstrated a long-term sustainable land-use by inhabitants of a large settlement occupied during the second half of the fourth millennium BC. The Middle Neolithic society established a settlement that covered an area of about 30–35 ha and functioned during at least three centuries, as validated by the absolute chronology. A thorough analysis based on the fuzzy-logic principle combined with archaeobotanical and archaeozoological data showed that the subsistence model was based on agricultural practices, related with both crop cultivation and animal husbandry, together with an important component of hunting, fishing and gathering. Ecological requirements of plants and animals represented by fossil remains suggested that the exploited area included a variety of habitats. In the open landscape, wet and dry grasslands, used partly as pastures, cultivated fields and marshes were present. Wooded areas included deciduous lime-oak-elm forests, also of open canopy type, and riparian forests. This was due to the transitional location of the Mozgawa site on a loess-mantled hill, covered with fertile soils, but right on the border with a vast alluvial plain of the Nida River. Such ecologically diversified micro-region became a managed landscape, which provided rich resources for seasonal diets of the settlement’s inhabitants. The case study of the Mozgawa site offers a unique insight into the palaeoeconomy of the Funnel Beaker culture in central Europe.