The Fission Yeast Actomyosin Cytoskeleton

Gachet, Yannick; Mulvihill, Daniel P.; Hyams, Jeremy S.

doi:10.1007/978-3-662-10360-9_14

Cited by 9 publications

(7 citation statements)

References 77 publications

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“…The dynamic behavior of F-actin patches and cables has been well documented during both the cell cycle and the life cycle of S. pombe (Marks et al, 1986;Petersen et al, 1998; reviewed by Gachet et al, 2004). In addition, colocalization of Myo1 with F-actin during the life cycle has been reported (Toya et al, 2001).…”

Section: Intracellular Localization Of Cam2 During the S Pombe Life mentioning

confidence: 99%

Localization of Type I Myosin and F-actin to the Leading Edge Region of the Forespore Membrane in Schizosaccharomyces pombe

Itadani

Nakamura

Shimoda

2006

Cell Struct. Funct.

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ABSTRACT. Myo1, a heavy chain of type I myosin of the fission yeast Schizosaccharomyces pombe, is essential for sporulation. Here we have analyzed the expression, localization and cellular function of the type I myosin light chain calmodulin, Cam2, encoded by cam2 + . Transcription of cam2 + was constitutive and markedly enhanced in meiosis. The cam2 null mutant was viable and completed sporulation normally at 28°C, but formed four-spored asci poorly at 34°C. In those sporulation-defective cells, the forespore membrane was formed abnormally. A Cam2-GFP fusion protein accumulated at the cell poles in interphase cells and at the medial septation site in postmitotic cells, colocalizing with Myo1 and F-actin patches. During the mating process, a single Cam2-GFP dot was detected at the tip of the mating projection. During meiosis-I, the Cam2-GFP dots dispersed into the cell periphery and the cytoplasm. At metaphase-II, intense Cam2-GFP signals appeared near Meu14 rings which were formed at the leading edge of expanding forespore membranes. This localization of Cam2 was dependent upon Myo1; and sporulation defect of cam2∆ at 34°C was alleviated by overexpressing Myo1∆IQ. These results suggest a close relationship between Cam2 and Myo1. In addition, both F-actin and Myo1 localized with Cam2 in the leading edge region. In summary, type I myosin and F-actin accumulate at the leading edge area of the forespore membrane and may play a pivotal role in its assembly.

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Section: Intracellular Localization Of Cam2 During the S Pombe Life mentioning

confidence: 99%

Localization of Type I Myosin and F-actin to the Leading Edge Region of the Forespore Membrane in Schizosaccharomyces pombe

Itadani

Nakamura

Shimoda

2006

Cell Struct. Funct.

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“…S. pombe uses both microtubules and the actin cytoskeleton for this purpose (for reviews, see Yarm et al, 2001;Chang and Verde, 2004;Gachet et al, 2004). S. pombe uses both microtubules and the actin cytoskeleton for this purpose (for reviews, see Yarm et al, 2001;Chang and Verde, 2004;Gachet et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Rgf1p Is a Specific Rho1-GEF That Coordinates Cell Polarization with Cell Wall Biogenesis in Fission Yeast

Garcia

Tajadura

García

et al. 2006

MBoC

111

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Rho1p regulates cell integrity by controlling the actin cytoskeleton and cell wall synthesis. We have identified a new GEF, designated Rgf1p, which specifically regulates Rho1p during polarized growth. The phenotype of rgf1 null cells was very similar to that seen after depletion of Rho1p, 30% of cells being lysed. In addition, rgf1(+) deletion caused hypersensitivity to the antifungal drug Caspofungin and defects in the establishment of bipolar growth. rho1(+), but none of the other GTPases of the Rho-family, suppressed the rgf1Delta phenotypes. Moreover, deletion of rgf1(+) suppressed the severe growth defect in rga1(+) null mutants (a Rho1-GAP, negative regulator). Rgf1p and Rho1p coimmunoprecipitated and overexpression of rgf1(+) specifically increased the GTP-bound Rho1p; it caused changes in cell morphology, and a large increase in beta(1,3)-glucan synthase activity. These effects were similar to those elicited when the hyperactive rho1-G15V allele was expressed. A genetic relationship was observed between Rgf1p, Bgs4p (beta[1,3]-glucan synthase), and Pck1p (protein kinase C [PKC] homologue); Bgs4p and Pck1p suppressed the hypersensitivity to Caspofungin in rgf1Delta mutants. Rgf1p localized to the growing ends and the septum, where Rho1, Pck1p, and Bgs4p are known to function. Our results suggest that Rgf1p probably activates the Rho functions necessary for coordinating actin deposition with cell wall biosynthesis during bipolar growth, allowing the cells to remodel their wall without risk of rupture.

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“…Following cell separation polarity must be re‐established at the old end. Fission yeast uses both microtubules and the actin cytoskeleton for cell expansion (for reviews, see14, 30, 125). It has been proposed that it is the interaction of microtubules with the cell tips that allows the establishment of new sites of actin assembly (reviewed by6, 13, 14, 38, 64, 109).…”

Section: The Role Of Cdc42p Scd1p and Gef1p In Cell Shapementioning

confidence: 99%

“…Cell growth transitions are correlated with changes in the actin cytoskeleton. The growing ends of fission yeast contain polarized cables and actin patches30, 63, while at cell division, actin patches disappear from the poles and the cytokinetic actomyosin ring (CAR) forms at the cell equator from a combination of the reorganization of interphase cables and de novo actin assembly1, 90. The relationship between actin and cell wall deposition at the cell poles and equator is still not well understood109.…”

Section: The Role Of Rho1p Rgf3p Rgf1p and Rgf2p In Maintaining Celmentioning

confidence: 99%

Role of Rho GTPases and Rho‐GEFs in the regulation of cell shape and integrity in fission yeast

Garcia

Tajadura

García

et al. 2006

Yeast

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The Rho family of GTPases are highly conserved molecular switches that control some of the most fundamental processes of cell biology, including morphogenesis, vesicular transport, cell division and motility. Guanine nucleotide-exchange factors (GEFs) are directly responsible for the activation of Rho-family GTPases in response to extracellular stimuli. In fission yeast, there are seven Dbl-related GEFs and they activate six Rho-type GTPases within a particular spatio-temporal context. The failure to do so might have consequences reflected in aberrant phenotypes and in some cases lead to cell death. In this review, we briefly summarize the role of Rho GTPases and Rho-GEFs in the establishment and maintenance of cell polarity and cell integrity in Schizosaccharomyces pombe.

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The Fission Yeast Actomyosin Cytoskeleton

Cited by 9 publications

References 77 publications

Localization of Type I Myosin and F-actin to the Leading Edge Region of the Forespore Membrane in Schizosaccharomyces pombe

Localization of Type I Myosin and F-actin to the Leading Edge Region of the Forespore Membrane in Schizosaccharomyces pombe

Rgf1p Is a Specific Rho1-GEF That Coordinates Cell Polarization with Cell Wall Biogenesis in Fission Yeast

Role of Rho GTPases and Rho‐GEFs in the regulation of cell shape and integrity in fission yeast

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