The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteins

Making the most of affinity tags

“…Although many different tags have been described in the literature, few of them have been tested in a high-throughput context. Peptide epitopes like the FLAG-tag [19], the calmodulin-binding peptide [20], the Strep-tag or Streptag II [21,22] and the biotin acceptor peptide [23] all exhibit a high degree of specificity for their cognate binding partners. However, the resins (immobilized proteins) that they interact with tend to be expensive, are easily fouled and have relatively low binding capacities, making them less than ideal for high-throughput applications.…”

One-by-One Sample Preparation Method for Protein Network Analysis

“…This automated system is compatible with the single-step affinity purification technique using the Flag-tag system (Einhauer & Jungbauer, 2001), without sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation. Affinity-purification is a technique for purification of physiological protein complexes using target proteins (bait proteins) fused with affinity tags, such as short epitope peptides (e.g., Flag and Myc) or tandem-affinity purification (TAP) tags (Kocher & Superti-Furga, 2007).…”

Overexpression of sorcin results in multidrug resistance in gastric cancer cells with up-regulation of P-gp

“…FLAG fusion proteins can be readily purified and assayed by ELISA or any other immunochemical detection method (26,27). Thus, we chose this affinity system for further studies of sorcin protein characterization.…”