The flavonoids extract from Okra flowers protects against DSS-induced colitis via regulating NF-κB signaling pathway and gut microbiota

Phenolics from Dendrobium officinale Leaf Ameliorate Dextran Sulfate Sodium-Induced Chronic Colitis by Regulating Gut Microbiota and Intestinal Barrier

“…Compared with the MC group, the tight junction of the DOPL group was significantly restored (Figure I–K). Previous studies have shown that dietary flavonoids and phenolic compounds have a positive regulatory effect on the increased levels of tight junction proteins. , Our experiments confirmed this idea and indicated that DOP may improve the intestinal barrier by enhancing the tight junction protein.…”

Rosa Roxburghii Tratt Fruit Extract Prevents Dss-Induced Ulcerative Colitis in Mice by Modulating the Gut Microbiota and the IL-17 Signaling Pathway

“…It is worth noting that the dilution concentration of RRTE in the ABTS-scavenging assay was up to 0, 10, 20, 40, 60, 80, 100, 120, 140, 160, 180, and 200 µg/mL, and -scavenging up to 100, 200, 300, 400, 500, 600, 700, 800, 900, and 1000 µg/mL in the DPPH-scavenging assay, which confirmed after pre-experiment. The human normal colon epithelial (NCM460) cells were purchased from ATCC (the United States) and cultured in DMEM medium containing 10% serum and 1% double antibody in a cell culture incubator at 37 • C with 5% CO 2 concentration [10].…”

“…NCM460 cells were planted in 96-well plates at 5 × 10 3 cells/well; and after wall attachment, we added RRTE at a concentration gradient of 200-500 µg/mL for 12/24 h, and then 90 mg/mL DSS solution for 12 h. After the intervention was completed, 20 µL of MTT solution at a concentration of 5 mg/mL was added; and after incubation for 2-4 h, the solution in the wells was aspirated, DMSO solution (150 µL/well) was added and the solution was shaken for 15 min to dissolve the blue-violet crystals at the bottom, and the absorbance at 570 nm was determined [10].…”

“…When 10,000 cell data were collected in the designated area, the collection was terminated to ensure that the number of cells in the designated area is constant during each collection. The statistical data of ROS expression are based on the area of fluorescence accumulation in the same size gate, which is the same as our previous method [10]. C with 40-60% humidity, 12 h of light/dark cycle, and free access to drinking water and food.…”

“…Natural products derived from herbs, medicinal plants and functional foods have been favored by researchers for their bioactivities such as anti-inflammatory, anti-oxidant, and immunomodulatory properties, combined with high safety and low cost [7][8][9]. Flavonoid extracts of okra flowers have been found to alleviate DSS-induced UC by protecting the intestinal barrier and modulating the NF-κB signaling pathway and intestinal microbial composition [10]; cherry polyphenol extract reduces oxidative damage and pro-inflammatory cytokine release, protects intestinal barrier function, and inhibit the Wnt/β-Catenin signaling pathway to protect from the UC [11]; the proprietary Chinese medicine Zhuling Jianpi capsule may rely on its flavonoids, terpenoids and other compounds to alleviate UC [12]. Therefore, natural products that are rich in polyphenols, flavonoids, and terpenoids may be promising alternative therapeutic agents for UC [13].…”