The follicle‐associated epithelium of the ileal Peyer's patch in ruminants is distinguished by its shedding of 50 nm particles

Landsverk, Thor

doi:10.1038/icb.1987.28

Cited by 55 publications

(35 citation statements)

References 12 publications

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“…ln a previous study it was reported that the FAE of the ileal PP in ruminants release 50 nm membrane-bounded particles from their lateral plasma membrane (1). We now report that CA is a selective enzyme marker for the membrane-bounded particles.…”

Section: Introductionmentioning

confidence: 67%

Carbonic anhydrase—a marker for particles shed from the epithelium to the lymphoid follicles of the ileal Peyer's patch in goat kids and lambs

Landsverk

Jansson

Nicander³

et al. 1987

Immunol Cell Biol

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Summary Carbonic anhydrase (CA) was found to be a marker for 50 nanometer membrane‐bounded particles shed from the lateral cell border of follicle‐associated epithelial cells (FAE) in the ileal Peyer's patch (PP) of pre‐ and post‐natal lambs and goat kids. The CA‐positive particles seemed to filter into the underlying lymphoid tissue where they formed part of the matrix embedding the cells of the follicle centre.

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Section: Introductionmentioning

confidence: 67%

Carbonic anhydrase—a marker for particles shed from the epithelium to the lymphoid follicles of the ileal Peyer's patch in goat kids and lambs

Landsverk

Jansson

Nicander³

et al. 1987

Immunol Cell Biol

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“…In sheep, the ileal Peyer's patch functions as an organ of post-rearrangement diversification (Reynaud et al, 1995;Weill and Reynaud, 1996) and is responsible for the vast majority of B-cell production (Reynolds and Morris, 1983;Gerber et al, 1986). The growth and function of the ileal Peyer's patch has been related to processes of epithelial differentiation (Landsverk, 1987;Landsverk et al, 1987Landsverk et al, , 1990 (Reynolds, 1985).…”

Section: Discussionmentioning

confidence: 99%

Development of Accessory Cells in B‐Cell Compartments Is Retarted in B‐Cell‐Depleted Fetal Sheep

Press

Reynolds

McClure

et al. 1997

Journal of Immunology Research

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(Halleraker et al., 1994). As sheep are only exposed to exogenous nonself antigens after birth because of the protection provided by a syndesmochorial placenta (Brambell, 1970;Boyd et al., 1976), it follows that factors other than exogenous nonself antigens are responsible for this modification.The interdependence of lymphoid-cell differentiation and stromal-cell development has been the subject of a number of studies. In man, rat and sheep, the early occurrence of FDCs was concomitant with B-cell aggregation and the appearance of primary follicles (Markgraf et al., 1982;Kroese et al., 1987;Halleraker et al., 1994 (Press et al., 1996) has allowed the investigation of the development of lymphoid and accessory-cell populations in a species shielded from exogenous antigen during gestation. In addition to FDCs in primary follicles, other accessory-cell populations that share a close association with B cells include the FDCs of gut-associated lymphoid follicles and macrophages of the marginal zone of the spleen. The aim of the present study was to characterize, using a panel of enzyme and immunohistochemical techniques, the accessory-cell populations in the lymphoid tissues of B-cell-depleted fetal sheep. RESULTS Ileal Peyer's Patch FolliclesThe small follicles in the ileal Peyer's patch of the Bcell-depleted fetuses demonstrated intense reactivity for the enzyme 5'nucleotidase (5'N), which was similar to the intensity of reactivity shown in the ileal Peyer's patch follicles of the untreated litter mates and the age-matched control fetuses (Figure 1). There was intense reactivity for magnesium-dependent adenosine triphosphatase (MgATPase) in some cells of the dome and interfollicular areas in the ileal Peyer's patch of the B-cell-depleted fetuses ( Figure 1). The dome region of ileal Peyer's patch of the Bcell-depleted fetuses showed increased reactivity for acid phosphatase whereas there was an absence of reactivity in the rudimentary follicle (Figure 1) There was also some reactivity for nonspecific esterase in the dome and interfollicular areas (not shown).A double immunofluorescence technique showed that the rudimentary follicles of the ileal Peyer' s patch in the B-cell-depleted fetuses showed strong staining for complement receptor-2 (CD21) and did not stain for IgM (not shown). Primary Follicles in the Spleen and Lymph NodesThe spleen and lymph nodes of the B-cell-depleted fetuses did not show the characteristic patterns of enzyme reactivity for 5'N, MgATPase, nonspecific esterase, and alkaline phosphatase that are associated with primary follicles (Figure 1). Serial step sectioning of the spleen and lymph nodes combined with staining for 5'N reactivity did not identify primary follicles in the B-cell-depleted fetuses. Primary follicles were readily identifiable by their typical pattern of enzyme reactivities in the control fetuses (Figure 1).The spleen and lymph nodes of B-cell-depleted fetuses contained a small population of CD21 cells.In the lymph nodes, most of these cells were scattered in the cortex, b...

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“…The surface of the dome region is covered by the follicle-associated epithelium (FAE) comprising M (microfold) cells (13), whose function is to selectively sample and transport luminal Ags, including proteins and microorganisms (14). The numbers of M cells vary between species, ranging from percentages as low as 2% of the FAE in humans (15) to an almost homogeneous population in the ruminant ileal Peyer's patch (16,17). The differentiation and uptake processes of M cells are poorly defined (18), mainly because their scarcity in the intestinal mucosa has hampered their cellular and biochemical studies.…”

Section: Peyer's Patches M Cells and Dendritic Cells (Dc) 3 In Intementioning

confidence: 99%

Roundtrip Ticket for Secretory IgA: Role in Mucosal Homeostasis?

Corthésy¹

2007

The Journal of Immunology

196

148

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An important activity of mucosal surfaces is the production of Ab referred to as secretory IgA (SIgA). SIgA serves as the first line of defense against microorganisms through a mechanism called immune exclusion. In addition, SIgA adheres selectively to M cells in intestinal Peyer’s patches, thus mediating the transepithelial transport of the Ab molecule from the intestinal lumen to underlying gut-associated organized lymphoid tissue. In Peyer’s patches, SIgA binds and is internalized by dendritic cells in the subepithelial dome region. When used as carrier for Ags in oral immunization, SIgA induces mucosal and systemic responses associated with production of anti-inflammatory cytokines and limits activation of dendritic cells. In terms of humoral immunity at mucosal surfaces, SIgA appears thus to combine properties of a neutralizing agent (immune exclusion) and of a mucosal immunopotentiator inducing effector immune responses in a noninflammatory context favorable to preserve local homeostasis of the gastrointestinal tract.

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The follicle‐associated epithelium of the ileal Peyer's patch in ruminants is distinguished by its shedding of 50 nm particles

Cited by 55 publications

References 12 publications

Carbonic anhydrase—a marker for particles shed from the epithelium to the lymphoid follicles of the ileal Peyer's patch in goat kids and lambs

Carbonic anhydrase—a marker for particles shed from the epithelium to the lymphoid follicles of the ileal Peyer's patch in goat kids and lambs

Development of Accessory Cells in B‐Cell Compartments Is Retarted in B‐Cell‐Depleted Fetal Sheep

Roundtrip Ticket for Secretory IgA: Role in Mucosal Homeostasis?

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