The formation of a new horseradish peroxidase binding rare earth

Jiang, Na; Wang, Lihong; Du, C.-R.; Ding, Xifeng; Huang, Xiaohua

doi:10.1007/s10311-009-0262-6

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…基于 CHARMm 力场 Steepest Descent 和 Conjugate Gradient 连续优化, 溶剂环境为水, 离子强度为 0.1 M. 用 Ramanchandran Plot 检验建模结果可靠性 [28] . 设定植物细胞质膜上和质膜外环境(pH 6.0~6.5), 对所构建 VN 分子模型进行分子动力学模拟并蛋白表面电荷分布计算 [29] . 选取蛋白表面负电荷集中区域一个氨基酸残基进行与稀土相互作用的量子化学计算.…”

Section: 量子化学计算unclassified

Different targets of lanthanum(III), cerium(III) and terbium(III) action on cells

Yang¹,

Wang²,

He³

et al. 2016

Sci. Sin.-Chim

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本文通过 140 La(III)、 141 Ce(III)、 160 Tb(III)在植物体内放射自显影技术和量子化学计算等研究发现, 化学性质相似的稀土离子 La(III)、Ce(III)、Tb(III)在细胞质膜和质膜外作用靶点显著不同, 即 Ce(III)主要与质膜物质发生键合, La(III)还能与细胞质膜外的细胞外物质和胞外基质分子键合, Tb(III)更可与细胞壁物质键合; La(III)和 Tb(III)与胞吞作用进入细胞的物质发生键合而进入细胞, Ce(III)尚未观察到此现象; La(III)和 Tb(III)与细胞质膜和质膜外物质通过配位共价键及分子间氢键形成稳定的路易斯酸碱配合物, Ce(III)则可能仅靠库仑力与细胞质膜物质发生作用. 由此导致 La(III)、Ce(III)、Tb(III) 3 种离子对细胞的作用靶点具有明显选择性, 其中 Ce(III)的选择性>La(III)>Tb(III). 可见, 3 种稀土离子不同数量 f 电子的成键活性所致细胞质膜及膜外靶分子键长、键角等参数差异, 将导致下游信号转导及细胞化学行为迥异, 终致其毒理不同. 本文结果可为科学探究稀土累积对生物危害的细胞学机理提供新的思考与借鉴, 也为稀土食品安全及人类健康预防提供重要指导.

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Section: 量子化学计算unclassified

Different targets of lanthanum(III), cerium(III) and terbium(III) action on cells

Yang¹,

Wang²,

He³

et al. 2016

Sci. Sin.-Chim

Self Cite

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Direct Interaction between Terbium Ion and Peroxidase in Horseradish at Different pH Values

Wang

Zhou

Huang

2014

Biol Trace Elem Res

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Rare earth elements (REEs) entering plant cells can directly interact with peroxidase in plants, which is the structural basis for the decrease in the activity of peroxidase. Different cellular compartments have different pH values. However, little information is available regarding the direct interaction between REEs and peroxidase in plants at different pH values. Here, we investigated the charge distribution on the surface of horseradish peroxidase (HRP) molecule as well as the interaction of terbium ion (Tb(3+), one type of REEs) and HRP at different pH values. Using the molecular dynamics simulation, we found that when the pH value was from 4.0 to 8.0, a large amount of negative charges were intensively distributed on the surface of HRP molecule, and thus, we speculated that Tb(3+) with positive charges might directly interact with HRP at pH 4.0-8.0. Subsequently, using ultraviolet-visible spectroscopy, we demonstrated that Tb(3+) could directly interact with HRP in the simulated physiological solution at pH 7.0 and did not interact with HRP in other solutions at pH 5.0, pH 6.0 and pH 8.0. In conclusion, we showed that the direct interaction between Tb(3+) and HRP molecule depended on the pH value of cellular compartments.

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Toxic effects of heavy metal terbium ion on the composition and functions of cell membrane in horseradish roots

Yang

Wang

Zhou

et al. 2015

Ecotoxicology and Environmental Safety

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The formation of a new horseradish peroxidase binding rare earth

Cited by 3 publications

References 9 publications

Different targets of lanthanum(III), cerium(III) and terbium(III) action on cells

Different targets of lanthanum(III), cerium(III) and terbium(III) action on cells

Direct Interaction between Terbium Ion and Peroxidase in Horseradish at Different pH Values

Toxic effects of heavy metal terbium ion on the composition and functions of cell membrane in horseradish roots

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