AbstractThe reported incidence of vaginal agenesis varies from 1 in 4,000 to 1 in 80,000 and individuals with congenital absence of vagina usually are first seen by a gynecologist at ages 14 to 15 years, when the absence of menses causes concern. A colon-introitus anastomosis was performed with interrupted absorbable sutures after opening peritoneum on müllerien dimple by sharp dissection using a guide inserted through the created perineo-abdominal tunnel. A rare case of vaginal agenesis with a septate uterus and normal tubes and ovaries is reported. Sigmoid vaginoplasty seemed the most appropriate procedure for the case and the continuity of the genital tract was well established as the patient menstruated vaginally after the operation.