The Formation of Blood Vessel After the Administration of the Plasmid Encoding Ang-1 Gene in Fischer Rats

Skóra, Jan; Płonek, Tomasz; Barć, Piotr; Baczyńska, Dagmara; Radwańska, Agata; Pupka, Artur; Korta, Krzysztof; Ussowicz, Marek; Stróżecki, Łukasz; Kupczyńska-Markiewicz, Diana; Hałoń, Agnieszka

doi:10.17219/acem/62430

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…Quartu et al [4] reported that the administration of frankincense essential oils (unsaturated fatty acids containing DHA and eicosapentaenoic acid) before cerebral ischemia can reduce the inflammatory response of brain tissue by downregulating the expression of COX-2 and provide nutritional support to damaged brain tissue, improving the cell survival rate and thus reducing cerebral ischemia/reperfusion injury. Paterniti et al [31] confirmed that DHA can significantly reduce inflammation and tissue damage after traumatic spinal cord injury. Further studies have shown that DHA can alleviate cerebral ischemia/reperfusion injury, maintain blood-brain barrier integrity, and reduce blood-brain barrier permeability [5].…”

Section: Discussionmentioning

confidence: 97%

“…Angiopoietins are proteins secreted by vascular endothelial cells, pericytes, myocytes, and mesenchymal cells. The competitive binding of Ang-1 and Ang-2 to Tie-2 plays an important role in blood-brain barrier permeability, endothelial cell fusion, and the response of blood vessels to different organ systems and disease states [31][32][33]. Further studies showed that downregulated Ang-1 expression is closely related to blood-brain barrier function after cerebral ischemia and that the administration of Ang-1 can alleviate injury to the blood-brain barrier induced by ischemia, while Ang-2 and VEGF cause damage blood-brain barrier permeability [34,35].…”

Section: Discussionmentioning

confidence: 99%

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DHA Attenuates Hypoxia/Reoxygenation Injury by Activating SSeCKS in Human Cerebrovascular Pericytes

Fang

Qiu

et al. 2019

Neurochem Res

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Docosahexaenoic acid (DHA) can alleviate cerebral ischemia/reperfusion injury by reducing blood-brain barrier permeability and maintaining its integrity, accompanied by an increased Ang-1/Ang-2 ratio; however, the underlying mechanisms of these effects remain unclear. Src-suppressed C kinase substrates (SSeCKS), a substrate of protein kinase C, plays an important role in maintaining cell junctions and cell morphology and regulating cell permeability. However, whether DHA can increase SSeCKS expression and then mediate the Ang-1/Ang-2 ratio still needs to be studied. Human cerebrovascular pericytes (HBVPs) cultured in vitro were divided into groups, treated with or without DHA along with SSeCKS siRNA to knockdown SSeCKS expression, and then subjected to 24 h of hypoxia followed by 6 h of reoxygenation. Cell viability; lactate dehydrogenase (LDH) release; and Ang-1, Ang-2 and VEGF activity were detected by using ELISA kits. The apoptosis rate was assessed by TUNEL flow cytometry. Expression of the SSeCKS, Ang-1, Ang-2 and VEGF proteins was evaluated by western blotting. Pretreatment with 10 μM or 40 μM DHA efficiently attenuated hypoxia/reoxygenation (H/R) injury by activating SSeCKS to increase the Ang-1/Ang-2 ratio and downregulate VEGF expression in HBVPs, as evidenced by decreased LDH release and apoptotic rates and increased HBVPs viability. Meanwhile, after we used SSeCKS siRNA to knock down SSeCKS protein expression, the protective effect of DHA on HBVPs following H/R injury was reversed. In conclusion, DHA can activate SSeCKS to increase the Ang-1/Ang-2 ratio and downregulate VEGF expression in HBVPs, thus reducing H/R injury.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

DHA Attenuates Hypoxia/Reoxygenation Injury by Activating SSeCKS in Human Cerebrovascular Pericytes

Fang

Qiu

et al. 2019

Neurochem Res

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“…The ANG-1 plasmid was prepared analogously. The whole process has already been described in detail [16].…”

Section: Preparation Of Plasmid Dnamentioning

confidence: 99%

Two-Stage Gene Therapy (VEGF, HGF and ANG1 Plasmids) as Adjunctive Therapy in the Treatment of Critical Lower Limb Ischemia in Diabetic Foot Syndrome

Barć

Antkiewicz

Frączkowska-Sioma

et al. 2022

IJERPH

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One of the most serious problems in people with diabetes is diabetic foot syndrome. Due to the peripheral location of atherosclerotic lesions in the arterial system of the lower extremities, endovascular treatment plays a dominant role. However, carrying out these procedures is not always possible and does not always bring the expected results. Gene therapy, which stimulates angiogenesis, improves not only the inflow from the proximal limb but also the blood redistribution in individual angiosomes. Due to the encouraging results of sequential treatment consisting of intramuscular injections of VEGF/HGF bicistronic plasmids followed by a month of ANG1 plasmids, we decided to use the described method for the treatment of critical ischemia of the lower limbs in the course of diabetes and, more specifically, in diabetic foot syndrome. Twenty-four patients meeting the inclusion criteria were enrolled in the study. They were randomly divided into two equal groups. The first group of patients was subjected to gene therapy, where the patients received intramuscular injections of pIRES/VEGF165/HGF plasmids and 1 month of ANG-1 plasmids. The remaining patients constituted the control group. Gene therapy was well tolerated by most patients. The wounds healed significantly better in Group 1. The minimal value of ABI increased significantly in Group 1 from 0.44 ± 0.14 (± standard deviation) to 0.47 ± 0.12 (with p = 0.028) at the end of the study. There were no significant differences in the control group. In the gene treatment group, PtcO2 increased significantly (from 28.71 ± 10.89 mmHg to 33.9 ± 6.33 mmHg with p = 0.001), while in Group 2, no statistically significant changes were found. The observed resting pain decreased significantly in both groups (Group 1 decreased from 6.80 ± 1.48 to 2.10 ± 1.10; p < 0.001; the control group decreased from 7.44 ± 1.42 to 3.78 ± 1.64 with p < 0.001). In our study, we evaluated the effectiveness of gene therapy with the growth factors described above in patients with CLI in the course of complicated DM. The therapy was shown to be effective with minimal side effects. No serious complications were observed.

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Angiogenic and Migratory Gene Expression Analysis of Stem Cells From Exfoliated Deciduous Teeth for Wound Repair Application

Aziz

Ahmad

Yusop

2022

CSCR

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Background: The migration and differentiation of stem cells take place during the reparative phase of the healing cascade. Chemokine ligands and receptors are the key players in the homing process dur-ing the early stage of capillary morphogenesis. Stem cells from exfo-liated deciduous teeth are known to possess a huge potential benefit for tissue regeneration. However, the gene expression of SHED en-gaging in angiogenesis and migratory activity during tissue healing is not fully understood. This study aims to assess the gene expression of SHED following in-vitro angiogenesis and migratory induction protocol. Methods: Scratch test assay was conducted following an angiogenic induction of SHED by supplementation of EGM-2 and VEGF. For the detection of migratory cell markers, angiogenic markers, and stem cell markers, RNA samples were extracted on day 1, 3, 7, 10, and 14 after the angiogenic induction in a transwell chamber, fol-lowed by RT-PCR analysis. Results: The findings suggested that SHED forming endothelial cells at higher capacity under an imma-ture state with higher seeding density. SHED undergoing angiogen-esis and migratory activity showed elevated IL-8, CCR1, CXCR4 and CCL28 expression. CCR1 expression significantly increased in the A+M+ group (p<0.05). Conclusion: The gene expression of these chemokines, particularly CCR1, which closely represent cellular migration, suggests the po-tential use of SHED for cell-based therapy to enhance tissue repair.

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The Formation of Blood Vessel After the Administration of the Plasmid Encoding Ang-1 Gene in Fischer Rats

Cited by 3 publications

References 23 publications

DHA Attenuates Hypoxia/Reoxygenation Injury by Activating SSeCKS in Human Cerebrovascular Pericytes

DHA Attenuates Hypoxia/Reoxygenation Injury by Activating SSeCKS in Human Cerebrovascular Pericytes

Two-Stage Gene Therapy (VEGF, HGF and ANG1 Plasmids) as Adjunctive Therapy in the Treatment of Critical Lower Limb Ischemia in Diabetic Foot Syndrome

Angiogenic and Migratory Gene Expression Analysis of Stem Cells From Exfoliated Deciduous Teeth for Wound Repair Application

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