The formation of granules in the bronchiolar Clara cells of the rat

Kuhn, Charles; Callaway, Lawrence A.; Askin, Frederic B.

doi:10.1016/s0022-5320(74)90052-5

Cited by 84 publications

(31 citation statements)

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“…?0.46 although their tinctorial properties have been a highly controversial issue. 46 T h e cells that lined the irregular spaces in one of our tumors possessed only very few membrane-limited dense granules, probably of lysosomal nature, large numbers of free ribosomes, and polysomes; however, neither their smooth endoplasmic reticulum nor the Golgi apparatus was prominently devel0ped.4~ These lining cells also lacked several of the characteristic features of granular pneumocytes, namely, the extensive Golgi apparatus, numerous multivesicular bodies, and, most significantly, the lamellar bodies. However, it is possible that the cells in our material repre- T h e prominent vasciilature of the tumors, as well ;IS the pronounced vascular sclerosis, are features which deserve further comment.…”

Section: Cancer April 1976mentioning

confidence: 68%

Peripheral pulmonary carcinoid tumors

Bonikos

Bensch

Jamplis

1976

Cancer

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The light microscopic and ultrastructural features of five asymptomatic peripheral carcinoids presented as distinct pulmonary solitary nodules are described. By conventional microscopy the tumors displayed a variety of histologic patterns, the most unusual one showing tumor cells embedded in a richly vascular hyalinized stroma and forming papillary structures or cystic spaces lined by low cuboidal cells which ultrastructurally bore a strong resemblance to intermediate or transitional forms between types I and II pneumocytes. A striking feature of these tumors was their rich vasculature associated with a marked perivascular sclerosis composed of basement membrane‐like material and collagen fibrils most likely produced by the increased numbers of pericytes surrounding these sclerotic vessels. The clinical implications, biologic behavior, and association of these tumors with other pulmonary neoplasms are also discussed.

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Section: Cancer April 1976mentioning

confidence: 68%

Peripheral pulmonary carcinoid tumors

Bonikos

Bensch

Jamplis

1976

Cancer

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“…PETRIK and CoLLET (1974) conceived the idea that Clara cells of the mouse bronchial tree secrete other substance rather than pulmonary surfactant. KUHN III et al (1974) maintained that some elements of bronchiolar lining were derived from the Clara cells, and EVANS et al (1978) suggested that rat Clara cells change to the other kinds of cells using the 3H-thymidine autoradiography. PACK et al (1980PACK et al ( , 1981 described Clara cells of the mouse trachea as having three kinds of secretory granules released by apocrine as well as by exocytotic machanism.…”

Section: The Formation Of Cyst-like Structures In Aged Micementioning

confidence: 99%

Fine structural aspects of the development and aging of the tracheal epithelium of mice.

Kawamata

Fujita

1983

Arch. Histol. Jap., Arch. Histol. Jpn

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Summary.The fine structure of the mouse tracheal epithelium from the fetal period to the stage of old age was examined with electron and light microscopes.The tracheal epithelium of the adult mouse is ciliated and single columnar in shape, consisting of three kinds of cells: ciliated, non-ciliated and basal.Neither goblet nor brush cells were found. The earliest signs of the cytodifferentiation of ciliated and non-ciliated cells appear at 15 and 17 days of gestation respectively. The secretory granules in non-ciliated cells appear at 17 days of gestation, and increase in number from 18 days of gestation (one day before birth) to 1 day after birth and decrease in number rapidly, while elements of smooth endoplasmic reticulum appear in this cell. Non-ciliated cells constitute the major component of the tracheal epithelium in the adult mouse. They have two kinds of granules: one a secretory granule surrounded by a single limiting membrane and released by exocytosis, and the other surrounded by double limiting membranes and considered to be derived from mitochondria. With the advance of age, some parts of the epithelium invaginate into the lamina propria without any noticeable changes in either the epithelial cells or the basal lamina. These invaginations, which the present authors named "cyst-like structures," are found in all mice over four months of age examined, and increase in number and size with age. They seem to be physiological structures and are distributed in all parts of the tracheal epithelium from the first tracheal ring to the tracheal bifurcation.They contain concentric circular materials with entangled filaments (15-20 nm in diameter) and/or destructed cell debris mainly derived from granular leucocytes.As early as 1956, RHODIN and DALHAMN classified epithelial cells of the rat trachea into four cell types: the ciliated, the goblet, the brush and the basal. This classification has been principally accepted for the rat (JEFFERY and REID,1975;MARIN et al., 1979) and for some other mammals.However, the tracheal epithelium of the mouse is quite different from that of these animals, being characterized by the occurrence of numerous non-ciliated cells and by the absence of goblet cells. The function and fine structural development of the non-ciliated cells are still obscure. The present paper deals with certain fine structural aspects of the differentiation, development, and the changes brought about through aging in the mouse tracheal epithelium from 14 days of gestation to advanced age. The cytodifferentiation of the ciliated cell and non-ciliated cell, and the aging of both cells are described through their fine structural aspects.

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“…They are located mainly in small conducting airways (3)(4), and are thought to secrete components of the extracellular lining of these airways (6). The extracellular lining of this region may be of particular importance because noxious agents inhaled into the lung commonly affect these airways (7), and because these airways are the site of obstruction early in the course of very common diseases such as chronic bronchitis and emphysema (8).…”

Section: Introductionmentioning

confidence: 99%

Regulation of secretion in Clara cells: studies using the isolated perfused rat lung.

Massaro¹,

Fischman²,

Chiang³

et al. 1981

J. Clin. Invest.

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A B S T R A C T Previous studies from our laboratory indicated that both beta-adrenergic and cholinergic agents stimulate in vivo secretion by rat bronchiolar Clara cells. Those studies also provided support for an in-series beta-adrenergic-cholinergic stimulation of secretion. To further explore the regulation of secretion in Clara cells, and to do it in the absence of systemic influences, we have used the isolated ventilated perfused rat lung. We have again used morphometry and electron microscopy to assess secretion by measuring the volume density (fraction of cell volume) of the secretory granules of bronchiolar Clara cells. We found that in the isolated perfused lung, as in the intact animal, isoproterenol stimulated secretion in Clara cells and that this effect was blocked by the betaadrenergic antagonist propranolol. Pilocarpine, unlike its action in the intact animal, did not stimulate secretion in the isolated lung; rather it inhibited the secretory effect of isoproterenol. Increased tidal-volume ventilation stimulated secretion; propranolol did not block this effect. Analogs of cyclic (c)AMP and of cGMP also stimulated secretion by Clara cells. These findings indicate that there are at least two mechanisms by which Clara cells can be stimulated to secrete. One seems to be beta-adrenergic-cAMP mediated but the triggering event is unknown. The other is initiated by increased tidal volume and cGMP may be involved in the intracellular mediation ofthis stimulatory event. Finally, we found evidence of beta-adrenergic

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The formation of granules in the bronchiolar Clara cells of the rat

Cited by 84 publications

References 17 publications

Peripheral pulmonary carcinoid tumors

Peripheral pulmonary carcinoid tumors

Fine structural aspects of the development and aging of the tracheal epithelium of mice.

Regulation of secretion in Clara cells: studies using the isolated perfused rat lung.

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