The Fourth Wave of Biocatalysis Emerges— The 13 th International Symposium on Biocatalysis and Biotransformations

Poppe, László; Vértessy, Beáta G.

doi:10.1002/cbic.201700687

Cited by 14 publications

(10 citation statements)

References 6 publications

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“…More and more synthetic technologies of bulk chemicals, including chiral alcohols and chiral amines, are shiing from traditional chemical synthesis processes to biosynthesis processes. [1][2][3][4] Principles for a green chemistry future are becoming increasingly more concrete, including low toxicity, recyclable, and easily separable green solvents or solventless, 5 and have been used to guide the synthesis of new organic molecules. 6 The commonly used reaction media mainly include various organic solvents, ionic liquids, and deep eutectic solvents for the enzymatic synthesis reaction.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic synthesis of 2-phenethyl acetate in water catalyzed by an immobilized acyltransferase from Mycobacterium smegmatis

Feng

Huang

et al. 2022

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Enzymatic synthesis of 2-phenethyl acetate in water catalyzed by an immobilized acyltransferase from Mycobacterium smegmatis

Feng

Huang

et al. 2022

RSC Adv.

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“…Sustainable and environmentally conscious development requires the advancement and application of economical, efficient, and green processes to meet the needs of different industries and users. Among the solutions suitable for the production of various materials to satisfy these needs, efficient catalytic technologies come to the fore, within which the ever-evolving biocatalytic processes play an important role [1,2]. In addition to the search for and development of newer and more efficient enzymes, the efficient development of biocatalysis requires that the mode of application of the biocatalysts should also be significantly improved [1,2].…”

Section: Introductionmentioning

confidence: 99%

Magnetically Agitated Nanoparticle-Based Batch Reactors for Biocatalysis with Immobilized Aspartate Ammonia-Lyase

et al. 2021

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In this study, we investigated the influence of different modes of magnetic mixing on effective enzyme activity of aspartate ammonia-lyase from Pseudomonas fluorescens immobilized onto epoxy-functionalized magnetic nanoparticles by covalent binding (AAL-MNP). The effective specific enzyme activity of AAL-MNPs in traditional shake vial method was compared to the specific activity of the MNP-based biocatalyst in two devices designed for magnetic agitation. The first device agitated the AAL-MNPs by moving two permanent magnets at two opposite sides of a vial in x-axis direction (being perpendicular to the y-axis of the vial); the second device unsettled the MNP biocatalyst by rotating the two permanent magnets around the y-axis of the vial. In a traditional shake vial, the substrate and biocatalyst move in the same direction with the same pattern. In magnetic agitation modes, the MNPs responded differently to the external magnetic field of two permanent magnets. In the axial agitation mode, MNPs formed a moving cloud inside the vial, whereas in the rotating agitation mode, they formed a ring. Especially, the rotating agitation of the MNPs generated small fluid flow inside the vial enabling the mixing of the reaction mixture, leading to enhanced effective activity of AAL-MNPs compared to shake vial agitation.

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“…Enzyme technology plays an important role in synthetic biology and biomanufacturing (Bornscheuer et al, 2012;Reetz, 2013;Sheldon and Brady, 2018). Developments in a variety of tools have resulted in the classical concept of biocatalysis expanding from single enzyme reactions to the incorporation of multiple enzymes allowing the assembly of synthetic biocatalytic pathways (Bornscheuer, 2018;Poppe and Vértessy, 2018). The current trend for "green" synthesis of chemicals has resulted in an increased focus on biocatalysis as an alternative to traditional pathways for sustainable and environmentally responsible biomanufacturing (Swartz, 2006(Swartz, , 2012Bornscheuer et al, 2012;France et al, 2017;Sperl and Sieber, 2018).…”

Section: Introductionmentioning

confidence: 99%

Cell-Free Biocatalysis for the Production of Platform Chemicals

2020

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Genetically engineered host bacteria have an extensive history for the production of specific proteins including the synthesis of single enzymes for the modification of compounds produced for industrial purposes by biological or chemical processes. Such processes have been developed largely through the process of discovery. The ability to assemble multiple enzymes into synthetic pathways is a new development aided by the synthetic biology approach of constructing and assembling suitable enzymes into pathways that may or may not occur in Nature to provide a highimpact platform for bio-manufacturing of chemicals, biofuels and pharmaceuticals. Industry has depended on chemical catalysts because of the known constraints experienced frequently with biological catalysts but when high stereochemistry, mild synthesis conditions and environmentally friendly processes are significant, application of enzymes is preferred, especially in the case of drug synthesis where enantioselectivity is important. However, many whole cell production processes are beset by toxicity problems, metabolite competition, the production of side products, sub-optimal enzyme ratios and varying temperature optima. As a result, a cell-free biocatalysis allows the manipulation of substrate ratios, provision of regenerated cofactors and adjustment of high energy flux ratios that are difficult or impossible to control in whole cell synthesis. We discuss here the construction of cell free biocatalytic pathways as added free enzymes or multi-enzyme modules that may contain heterologous catalysts. We examine the status of applications leading to commercialisation, emphasizing the importance of economical cofactor regeneration. Nevertheless, problems remain, particularly protein post-translational modifications including glycosylation, phosphorylation, ubiquitination, acetylation, proteolysis, etc. The National Renewable Energy Laboratory and Pacific North West Laboratory have published lists of top value-added chemicals from biomass that include glucaric acid. There have been few reports on the combination of synthetic biology and cell-free protein synthesis to set up pathways for these valuable intermediate compounds. This observation is despite the existence of at least one large scale but specialized cell-free production of antibody conjugates. This review will provide a description of one successful attempt at the cell-free production of glucaric acid and will evaluate progress for other key intermediate and platform chemicals.

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The Fourth Wave of Biocatalysis Emerges— The 13 th International Symposium on Biocatalysis and Biotransformations

Cited by 14 publications

References 6 publications

Enzymatic synthesis of 2-phenethyl acetate in water catalyzed by an immobilized acyltransferase from Mycobacterium smegmatis

Enzymatic synthesis of 2-phenethyl acetate in water catalyzed by an immobilized acyltransferase from Mycobacterium smegmatis

Magnetically Agitated Nanoparticle-Based Batch Reactors for Biocatalysis with Immobilized Aspartate Ammonia-Lyase

Cell-Free Biocatalysis for the Production of Platform Chemicals

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