The frequency and characteristics of highly thermophilic bacteria in cool soil environments

Marchant, Roger; Banat, Ibrahim M.; Rahman, Thahira; Berzano, Marco

doi:10.1046/j.1462-2920.2002.00344.x

Cited by 100 publications

(114 citation statements)

References 14 publications

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“…Development of thermophilic CO uptake in Canopy material but not Bare cinders might also reflect differences in CO oxidizer diversity, population sizes and resource availability for growth and metabolism, all of which increase from Bare cinders to Canopy material (King and Weber, 2008;Weber and King, 2010). Although thermophiles occur as dormant forms in many soils (Marchant et al, 2002(Marchant et al, , 2008Rahman et al, 2003), higher total microbial and CO-oxidizer diversity and population sizes in Canopy material might increase the likelihood that one or more thermophiles becomes active with elevated temperature. Similarly, higher organic matter and nitrogen contents of Canopy material might facilitate activation or growth.…”

Section: Discussionmentioning

confidence: 99%

Temperature responses of carbon monoxide and hydrogen uptake by vegetated and unvegetated volcanic cinders

King

2012

The ISME Journal

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Ecosystem succession on a large deposit of volcanic cinders emplaced on Kilauea Volcano in 1959 has resulted in a mosaic of closed-canopy forested patches and contiguous unvegetated patches. Unvegetated and unshaded surface cinders (Bare) experience substantial diurnal temperature oscillations ranging from moderate (16 1C) to extreme (55 1C) conditions. The surface material of adjacent vegetated patches (Canopy) experiences much smaller fluctuations (14-25 1C) due to shading. To determine whether surface material from these sites showed adaptations by carbon monoxide (CO) and hydrogen (H 2 ) consumption to changes in ambient temperature regimes accompanying succession, we measured responses of CO and H 2 uptake to short-term variations in temperature and long-term incubations at elevated temperature. Based on its broader temperature optimum and lower activation energy, Canopy H 2 uptake was less sensitive than Bare H 2 uptake to temperature changes. In contrast, Bare and Canopy CO uptake responded similarly to temperature during short-term incubations, indicating no differences in temperature sensitivity. However, during extended incubations at 55 1C, CO uptake increased for Canopy but not Bare material, which indicated that the former was capable of thermal adaptation. H 2 uptake for material from both sites was completely inhibited at 55 1C throughout extended incubations. These results indicated that plant development during succession did not elicit differences in short-term temperature responses for Bare and Canopy CO uptake, in spite of previously reported differences in CO oxidizer community composition, and differences in average daily and extreme temperatures. Differences associated with vegetation due to succession did, however, lead to a notable capacity for thermophilic CO uptake by Canopy but not Bare material.

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Section: Discussionmentioning

confidence: 99%

Temperature responses of carbon monoxide and hydrogen uptake by vegetated and unvegetated volcanic cinders

King

2012

The ISME Journal

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“…Morphological and biochemical characterization of the isolates designated F10 (from the meadow site) and Tf T (from the wooded site) were carried out using standard methods as described by Marchant et al (2002b). In order to allow longer incubation times for the biochemical tests, the cultures were incubated at 60 u C rather than at the higher temperatures of 60-70 u C at which the organisms are able to grow.…”

Section: Many Of the Organisms Previously Classified In The Genusmentioning

confidence: 99%

“…In order to allow longer incubation times for the biochemical tests, the cultures were incubated at 60 u C rather than at the higher temperatures of 60-70 u C at which the organisms are able to grow. DNA was extracted from pure cultures of the organisms and the 16S rRNA gene sequences were determined as described by Marchant et al (2002b). DNA extraction from cultured bacteria was performed using a BIO 101 Inc.…”

Section: Many Of the Organisms Previously Classified In The Genusmentioning

confidence: 99%

“…Three forward and three reverse sequencing primers were used (Marchant et al, 2002b). The sequencing reactions were carried out and analysed using the ABI Prism 3100 Genetic Analyzer system as specified by the manufacturer.…”

Section: Many Of the Organisms Previously Classified In The Genusmentioning

confidence: 99%

“…Although there are references to these thermophilic bacilli producing chains of cells, some isolates produce enormously long, undivided flexuous cells (Marchant et al, 2002b). In the case of G. debilis, no elongated cells have been observed and the rods were 0?5-1 mm wide by 1-14 mm long.…”

Section: Many Of the Organisms Previously Classified In The Genusmentioning

confidence: 99%

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Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov.

Banat

Marchant

Rahman

2004

International Journal of Systematic and Evolutionary Microbiology

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High growth rate and substrate exhaustion results in rapid cell death and lysis in the thermophilic bacterium Geobacillus thermoleovorans

Pavlostathis

Marchant

Banat³

et al. 2006

Biotech & Bioengineering

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Batch cultures of the thermophilic bacterium Geobacillus thermoleovorans T80 attained extremely high-specific glucose utilization rates leading to high specific growth rates, followed by extensive cell death and lysis with the onset of substrate exhaustion. The dramatic decrease in live cell numbers, as determined by flow cytometry, was accompanied by the release of soluble protein. Once the growth phase reached the point of commitment to lysis created by the impending exhaustion of substrate, the addition of extra carbon substrate did not halt the rapid death rate and lysis, although, towards the end of the exponential growth phase, the substrate was utilized producing only a small additional biomass concentration as a result of the net effect of cell growth and death. This lytic phenomenon was observed when a range of different carbon substrates (glucose, pyruvate, acetate, n-hexadecane, nutrient broth), as well as ammonium (the nitrogen source) in the presence of excess carbon source, reached near exhaustion. The rate and extent of cell death and the ensuing lysis depend on the culture growth rate. Cultures batch grown with a lower initial substrate concentration, or at a lower temperature, or at lower dilution rates for continuous-flow cultures, exhibited a lower rate and extent of cell death and lysis. Batch re-culture of the persister cells resulted in a behavior identical to that of the original culture indicating that these cells were not genetically modified. The glucose utilization, cell growth and death rates were mathematically described based on Monod kinetics and estimated values of pertinent biokinetic constants are reported.

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The frequency and characteristics of highly thermophilic bacteria in cool soil environments

Cited by 100 publications

References 14 publications

Temperature responses of carbon monoxide and hydrogen uptake by vegetated and unvegetated volcanic cinders

Temperature responses of carbon monoxide and hydrogen uptake by vegetated and unvegetated volcanic cinders

Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov.

High growth rate and substrate exhaustion results in rapid cell death and lysis in the thermophilic bacterium Geobacillus thermoleovorans

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