The frequency of high-risk human papillomavirus types, HPV16 lineages, and their relationship with p16INK4a and NF-κB expression in head and neck squamous cell carcinomas in Southwestern Iran

Pakdel, Fatemeh; Farhadi, Ali; Pakdel, Tahereh; Andishe-Tadbir, Azadeh; Alavi, Parnian; Behzad-Behbahani, Abbas; Ashraf, Mohammad Javad

doi:10.1007/s42770-020-00391-1

Cited by 8 publications

(7 citation statements)

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“…For all studies there was a significant difference in p16INK4a expression between HNSCC HPV(−) and HNSCC HPV16(+). Pakdel et al 2020 observed the expression of p16INK4a among HPV variants and showed that a strong p16INK4a expression was present in poorly differentiated tumour tissues infected with HPV16 sublineage A2 [ 45 ].…”

Section: Main Textmentioning

confidence: 99%

“…The authors remarked that the slight predominance of European variants accompanied observation from their normal cervical samples, suggesting that the distribution of HPV16 variants reflects the overall frequency in each studied population. The most recent study was published by Pakdel et al [ 45 ]. Thirteen HNSCC tissue specimens tested positive for HPV16 using overlapping PCR assays and were analyzed for the presence of E6 variants.…”

Section: Main Textmentioning

confidence: 99%

“…HPV genotyping was performed using different methods. Two studies using a commercial kit (INNO LIPA) which identifies 28 different HPV types (6,11,16,18,26,31,33,35,39,40,43,44,45,51,52,53,54,56,58,59, 66, 68, 69, 70, 71, 73, 74, 82) by reverse blot hybridization. Two other studies used TaqMan PCR methods targeting the E6 or E6/LCR.…”

Section: Hpv Detection and Genotypingmentioning

confidence: 99%

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Exploring the roles of HPV16 variants in head and neck squamous cell carcinoma: current challenges and opportunities

Cochicho

Costa

Félix

2021

Virol J

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The incidence of squamous cell carcinomas of the head and neck (HNSCC) is consistently increasing, in association with human papillomavirus (HPV) infection, especially HPV16. HPV variants show heterogeneity in the pathogenicity of cervical cancer, but little has been established about their relevance on HNSCC. This review addresses the distribution of HPV16 variants in HNSCC and their potential contribution to clinical practice. A search was performed in PubMed using the keywords HNSCC HPV16 variants. Sixty articles were identified between 2000 and 2020 and 9 articles were selected for a systematic analysis. Clinical cohorts comprised 4 to 253 patients aged between 17 and 91 years with confirmed HPV16-positive HNSCC. Samples were collected from fresh biopsies of the tumour, oral rinse or formol fixed/paraffin embedded tissue, from the oral cavity, oropharynx, hypopharynx, larynx and Waldeyer's tonsillar ring. HPV16 variants were identified using Sanger sequencing techniques. Seven studies addressed the HPV16 E6 gene, one studied E6 and E7, another studied L1 and one focused on the long control region. European variants represent 25–95%, Asian-American 5–57% and African 2–4% of the total isolates, suggesting a marked predominance of European strains. No correlations could be drawn with patient prognosis, partly because many studies relied on small patient cohorts. Additional studies are needed, particularly those employing next generation sequencing techniques (NGS), which will allow faster and accurate analysis of large numbers of samples.

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Section: Main Textmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

Section: Hpv Detection and Genotypingmentioning

confidence: 99%

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Exploring the roles of HPV16 variants in head and neck squamous cell carcinoma: current challenges and opportunities

Cochicho

Costa

Félix

2021

Virol J

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“…NF-κB dysregulation, among others, is a pivotal step in the occurrence and development of many tumors. The expression of NF-κB is upregulated in several cancers, including head and neck squamous cell carcinoma (HNSCC), pancreatic ductal adenocarcinoma, colorectal cancer, and gastric cancer [ 60 , 61 ]. The expression and activation of p65, the most well-studied NF-kB subunit in cancer, is increased in RCC tissues [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Epstein–Barr virus infection is associated with the nuclear factor-kappa B p65 signaling pathway in renal cell carcinoma

et al. 2022

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Background There have been few studies regarding viral involvement in the pathogenesis of renal cell carcinoma (RCC). The aim of this study was to examine the possible association of Epstein–Barr virus (EBV) infection with clinicopathological features and cellular biomarkers including p53, p16INK4a, Ki-67 and nuclear factor-kappa B (NF-κB) in RCC tumors. Methods In this prospective study, 122 histologically confirmed Formalin-fixed Paraffin-embedded RCC tissue specimens along with 96 specimens of their corresponding peritumoral tissues and 23 samples of blunt renal injuries were subjected to nested polymerase chain reaction (nPCR) in order to amplify EBV DNA sequences. The expression of p53, p16INK4a, Ki-67 and NF-κB was investigated by immunohistochemistry (IHC) assay. Statistical analysis was employed to demonstrate the possible associations. Results Infection with EBV was found to be significantly associated with RCC. Our results indicate that p65 NF-κB signaling pathway is probably involved in EBV-mediated RCC pathogenesis. Moreover, we found p53, Ki-67 and cytoplasmic NF-κB expression to be associated with tumor nuclear grade in RCC patients. The expression of p53 and Ki-67 was associated with primary tumor category as well. In addition, p53 overexpression was significantly more frequent among nonconventional RCC tumors than the conventional histologic type. Conclusions Infection with EBV is likely to play an important role in the development of RCC through the constitutive and permanent activation of NF-κB p65 signaling pathway. However, more experiments and supporting data are required to reach a decisive conclusion.

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“…The aberrant expression of this two groups of miRNAs, might gradually transform the normal cervix to a cancerous state in the case of HPV16 infection, whose transcription factors were predicted NF-κB family mainly. According to many studies in this area, NF-κB family was confirmed to be interfered by HPV infection [ 16 ]. However, most founding of these studies focus on the regulation of HPV-16 E6 and E7 on NF-κB expression, which always present conflicting data whether HPV-16 E6 and E7 stimulate or suppress NF-κB activation [ 10 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

Transcription factor c-Rel regulated by E5 affects the whole process after HPV16 infection through miR-133a-modulated feedback loop aim at mir-379-369 cluster

Zhou

et al. 2022

Cancer Cell Int

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Background During the development of cervical cancer, HPV infection causes a series of changes in transcription factors and microRNAs. But their relationships with pathogenic processes are not clear. Methods Base on previous study, to analyse the relationship among HPV16 infection and the related transcription factors, related miRNAs, so as to further understand the molecular mechanism of HPV16 infection to cervical cancer, around the HPV16 related miRNAs we have reported, the methods of bioinformatics prediction, histology, cell model in vitro and molecular interaction were used for prediction and validation respectively Results The results showed that NF-κB family members(c-Rel, p65 and p50) were identified as main HPV16rmiR-transcription factors. They have different expressive characteristics in cervical lesions and play tumorigenesis or progression roles in different periods of HPV16 infection. c-Rel, p65 and p50 act as mediators which link the HPV16 E5 and HPV16 related miRNAs. Among them, c-Rel affects the occurrence and progression of cervical cancer during whole HPV16 infection stage through miR133a-3p–modulated mir-379-369 cluster with a positive feedback way which targeted c-Rel itself and its positive regulator AKT3. Conclusion So in the course of HPV16 infection, the E5, c-Rel, and miR-133a-3p form a positive feedback system which aim at mir-379-369 cluster for the whole process from HPV16 infection to cervical cancer.

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The frequency of high-risk human papillomavirus types, HPV16 lineages, and their relationship with p16INK4a and NF-κB expression in head and neck squamous cell carcinomas in Southwestern Iran

Cited by 8 publications

References 58 publications

Exploring the roles of HPV16 variants in head and neck squamous cell carcinoma: current challenges and opportunities

Exploring the roles of HPV16 variants in head and neck squamous cell carcinoma: current challenges and opportunities

Epstein–Barr virus infection is associated with the nuclear factor-kappa B p65 signaling pathway in renal cell carcinoma

Transcription factor c-Rel regulated by E5 affects the whole process after HPV16 infection through miR-133a-modulated feedback loop aim at mir-379-369 cluster

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