The Frequency of imp and vim Genes Among Pseudomonas aeruginosa Isolates From Children’s Medical Center of Tehran

Bejestani, Fatemeh Bagheri; Hakemi-Vala, Mojdeh; Momtaheni, Raheleh; Bejestani, Ozra Bagheri; Goli, Hamid Reza

doi:10.5812/archcid.20991

Cited by 4 publications

(5 citation statements)

References 12 publications

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“…In the present study, similar to several studies in Iran, bla VIM and bla IMP have been only carbapenemase genes that were identified in carbapenem-resistant isolates of P. aeruginosa [ 3 , 16 , 17 ]. Also, our study, was not in agreement with Hakemi Vala et al and Bagheri Bejestani studies in Tehran, Iran, that reported no bla VIM positive P. aeruginosa isolates from different clinical samples such as burns’ patients [ 18 , 19 ]. However, bla VIM rates vary greatly among different studies [ 16 – 19 ] which may reflect differences in infection control policies and other factors.…”

Section: Discussioncontrasting

confidence: 97%

“…Also, our study, was not in agreement with Hakemi Vala et al and Bagheri Bejestani studies in Tehran, Iran, that reported no bla VIM positive P. aeruginosa isolates from different clinical samples such as burns’ patients [ 18 , 19 ]. However, bla VIM rates vary greatly among different studies [ 16 – 19 ] which may reflect differences in infection control policies and other factors.…”

Section: Discussioncontrasting

confidence: 97%

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Determination of carbapenem resistance mechanism in clinical isolates of Pseudomonas aeruginosa isolated from burn patients, in Tehran, Iran

Mirsalehian¹,

Kalantar-Neyestanaki²,

Taherikalani³

et al. 2017

JEGH

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Carbapenems are the most important therapeutic options that effect against serious infections caused by multidrug resistant Pseudomonas aeruginosa (MDR-PA) isolates. Carbapenems resistant isolates of P. aeruginosa are increasing worldwide. The aim of this study was to determine the carbapenem resistance mechanisms in clinical P. aeruginosa isolates from burn patients, in Tehran, Iran. A total of 53 non-duplicated isolates of carbapenem-resistant P. aeruginosa were collected from burn patients. The presence of carbapenemase genes were determined by PCR. AmpC overproducer isolates were detected by phenotypic method. The mutation and transcription level of oprD were determined by PCR-sequencing and quantitative Real-time PCR (RT-PCR), respectively. Twenty-seven (50.9%) isolates were positive for carbapenemase (bla=25 and bla=2) and showed high-level resistance to imipenem and meropenem. Twenty-eight isolates were AmpC overproducers. All isolates had a mutation in the oprD gene and down-regulation of oprD was found in 56.6% of MDR-PA isolates. Although the presence of carbapenemase is the common mechanism of resistant to carbapenem, but carbapenem resistance was found by oprD mutation-driven and the AmpC overproducing isolates in Tehran, Iran.

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Section: Discussioncontrasting

confidence: 97%

Section: Discussioncontrasting

confidence: 97%

Determination of carbapenem resistance mechanism in clinical isolates of Pseudomonas aeruginosa isolated from burn patients, in Tehran, Iran

Mirsalehian¹,

Kalantar-Neyestanaki²,

Taherikalani³

et al. 2017

JEGH

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“…In our study, 1.9% of the imipenem non-susceptible isolates of P. aeruginosa carried VIM-type MBLs. Bagheri Bejestani et al reported that bla VIM gene was not detected among the studied isolates in the center of Iran (27). However, several previous reports revealed that the genes of bla IMP and bla VIM -type MBL are often encoded on mobile genetic cassettes, which can easily spread among isolates.…”

Section: Discussionmentioning

confidence: 90%

Detection of Metallo-Beta-Lactamase-Encoding Genes Among Clinical Isolates of Pseudomonas aeruginosa in Qom Province

Meimani

Nazari

Rostami

2018

Zahedan J Res Med Sci

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Background: Carbapenem-resistant Pseudomonas aeruginosa has emerged as a serious problem in many hospitals and intensive care units. The purpose of this study was to determine the antibiotic susceptibility profile and the distribution of metallo-betalactamase genes among P. aeruginosa isolates from Qom province of Iran. Methods: In this study, 200 P. aeruginosa isolates were collected from several clinical samples in hospitals affiliated to Qom University of Medical Sciences, Qom province, Iran. The antibiotic susceptibility of the isolates was tested by the Kirby-Bauer disc diffusion test and the distribution of MBL genes among carbapenem-resistant isolates was determined by polymerase chain reaction (PCR) method. Results: Among carbapenem non-susceptible isolates of P. aeruginosa, 38% (76 isolates) were multidrug resistant, 26% (52 isolates) were pan-drug-resistant and 5% (10 isolates) were extensive drug resistant. 52% of the isolates were resistant to carbapenems. Among carbapenem non-susceptible isolates of P. aeruginosa, 40 isolates (38.4%) exhibited MBL production. Of 40 MBL-producing isolates, 38 isolates (36.5%) contained the blaIMP gene and two (1.9%) isolates contained the blaVIM gene. Conclusions: Outbreaks of MBL-producing P. aeruginosa isolates will be a serious problem in hospitals in the future and rapid identification of these isolates is necessary to control further dissemination of MBL resistance genes. This is the first report of the rates of MDR, XDR, PDR, and MBL-producing P. aeruginosa isolated from hospitals affiliated to Qom University of Medical Sciences in Qom province of Iran.

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“…Most samples were clinical urine specimens. Only MBL-associated genes including IMP and VIM were evaluated, 3.3% and 0%, respectively [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…In only one of these studies, the positivity rate of VIM gene was above 50%, and in other studies, it was between 0% and 47%. The frequency rate of imp gene was thoroughly various, between 0% and 100% [ 4 , 9 , 11 , 12 , 16 , 21 , 23 , 25 , 45 – 55 ].…”

Section: Discussionmentioning

confidence: 99%

Antipseudomonal β-Lactams Resistance in Iran

Rabiei

Asadi

Shokouhi

et al. 2020

International Journal of Microbiology

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Over the last years, the mortality rate of Pseudomonas aeruginosa, which is one of the major reasons for severe infections, has been significantly increasing. This bacterium is highly resistant to many antibiotics, especially carbapenems, thanks to its complicated mechanism by which it can acquire exogenous genes. The purpose of this research is to have a review of empirical studies surveying the P. aeruginosa resistance to beta-lactams in Iran in order to investigate the most reliable methods by which the incidence of P. aeruginosa infections can be decreased and controlled. We performed a systematic review of all articles published from 2008 until 2018. Studies which did not address P. aeruginosa resistance to beta-lactams were excluded from the analysis. Studies with less than 10 cases were also excluded. Studies with more than ten cases, which did not have repetitive information, were taken into account for the final selection; 133 out of 893 articles were chosen. The resistance rate of P. aeruginosa among the articles was as follows: more than 72% of studies revealed >50% level of resistance to cefepime, followed by aztreonam (53.2%), ceftazidime (61%), piperacillin/tazobactam (54.5%), meropenem (48.3%), and imipenem (42.4%). The selection of empiric antipseudomonal antibiotics is absolutely uncertain and hazardous, and the risk of clinical failure may be more among cephalosporins and piperacillin-tazobactam as well as aztreonam. The results of this study illustrate that the methods enabling clinics to identify the bacterium resistance pattern and its genetic basis and to have the opportunity of empiric therapies through access to updated local data of antimicrobial susceptibility pattern are the most effective methods. However, the widespread usage of these approaches undoubtedly needs reliable molecular and nucleic acid-based devices, which are both affordable and available.

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The Frequency of imp and vim Genes Among Pseudomonas aeruginosa Isolates From Children’s Medical Center of Tehran

Cited by 4 publications

References 12 publications

Determination of carbapenem resistance mechanism in clinical isolates of Pseudomonas aeruginosa isolated from burn patients, in Tehran, Iran

Determination of carbapenem resistance mechanism in clinical isolates of Pseudomonas aeruginosa isolated from burn patients, in Tehran, Iran

Detection of Metallo-Beta-Lactamase-Encoding Genes Among Clinical Isolates of Pseudomonas aeruginosa in Qom Province

Antipseudomonal β-Lactams Resistance in Iran

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