The frequency of TP53 R72P and MDM2 309T>G polymorphisms in Iranian infertile men with spermatogenetic failure: A case-control study

Abstract

Background:Tumor protein p53 (TP53) is a tumor suppressor transcriptional regulator protein which plays a critical role in the spermatogenesis. One of the most important regulators of p53 is Murine double minute 2 (MDM2), which acts as a negative regulator of the p53 pathway. Based on the key role of p53 and MDM2 in germ cell apoptosis, polymorphisms that cause a change in their function might affect germ cell apoptosis and the risk of male infertility.Objective:This study was designed to examine associations of TP53 72 Arg>Pro (rs1042522), and MDM2 309 T>G (rs937283) polymorphisms with spermatogenetic failure in Iranian population.Materials and Methods:A case-control study was conducted with 150 nonobstructive azoospermia or severe oligozoospermia and 150 fertile controls. The two polymorphisms, 72 Arg>Pro in TP53 and 309 T>G in MDM2, were genotyped using PCR-RFLP and ARMS-PCR respectively. Results:Our analyses revealed that the allele and genotype frequencies of the TP53 R72P polymorphism were not significantly different between the cases and controls (p=0.41, p=0.40 respectively). Also, no significant differences were found in the allelic (p=0.46) and genotypic (p=0.78) distribution of MDM2 309 T>G polymorphism between patients and controls.Conclusion:The results of this study indicate that polymorphisms of TP53 and MDM2 genes are unlikely to contribute to the pathogenesis of male infertility with spermatogenetic failure.

Formation of autotriploid Carassius auratus and its fertility-related genes analysis

“…In human, P53 gene polymorphism is higher in infertile men compared with fertile men, implying that it may affect germ cell apoptosis and increase risk of male infertility [29,32]. In the current study, analysis of expression levels of the TP53 gene showed signi cantly different expression levels between M3nRR and M4nRR.…”

Formation of autotriploid Carassius auratus and its fertility-related genes analysis

“…In addition, TP53 codon 72 polymorphism in mice is involved in meiosis, implying that it plays a critical role in spermatogenesis [ 31 ]. In human, P53 gene polymorphism is higher in infertile men compared with fertile men, implying that it may affect germ cell apoptosis and increase risk of male infertility [ 29 , 32 ]. In the current study, analysis of expression levels of the TP53 gene showed significantly different expression levels between M3nRR and M4nRR.…”