The full-length nucleotide sequences of the virulent Trinidad donkey strain of Venezuelan equine encephalitis virus and its attenuated vaccine derivative, strain TC-83

Kinney, Richard M.; Johnson, Barbara J. B.; Welch, Jacqueline; Tsuchiya, Kiyotaka R.; Trent, Dennis W.

doi:10.1016/0042-6822(89)90347-4

Cited by 158 publications

(144 citation statements)

References 43 publications

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“…Reverse transcription was carried out in a 20-L reaction containing 1 M of antisense primer VEE-9207B (5Ј-TRCACTGGCTGAACTGTT-3Ј), designed to anneal to 5Ј genomic position 9207 of the Trinidad donkey strain. 26 We included 1ϫ first strand buffer (Gibco BRL), 1 mM dNTPs, 80 U RNAsin (Promega), and 200 U SuperScript II reverse transcriptase (Gibco BRL) in the reaction. The cDNA was synthesized by incubating at 40ЊC for 20 min, followed by incubation at 50ЊC for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Genetic diversity and relationships among Venezuelan equine encephalitis virus field isolates from Colombia and Venezuela.

Moncayo

Medina

Kalvatchev

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. During field studies of enzootic Venezuelan equine encephalitis (VEE) viruses associated with epizootic emergence, a large number of virus isolates were made in sylvatic foci of Venezuela and Colombia. To rapidly characterize these isolates, antigenic subtypes were determined by means of immunofluorescence and by single-strand conformational polymorphism (SSCP) analysis by use of an 856-bp fragment from the P62 gene, which we used to distinguish genetic variants. Representative isolates were sequenced to assess the sensitivity of SSCP to detect genetic differences. The SSCP analysis distinguished isolates differing by as little as 1 nucleotide; overall, differences of Ն 1 nucleotide were recognized 89% of the time, and the sensitivity to distinguish strains that differed by only 1 or 4 nucleotides was 17 and 57%, respectively. Phylogenetic analyses of representative sequences showed that all recent isolates from the Catatumbo region of western Venezuela and the middle Magdalena Valley of Colombia were closely related to epizootic subtype IAB and IC strains; strains from Yaracuy and Miranda States were more distantly related. Cocirculation of the same virus genotype in both Colombian and Venezuelan foci indicated that these viruses are readily transported between enzootic regions separated by Ͼ 300 km. The SSCP analysis appears to be a simple, fast, and relatively efficient method of screening VEE virus isolates to identify meaningful genetic variants.

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Section: Methodsmentioning

confidence: 99%

Genetic diversity and relationships among Venezuelan equine encephalitis virus field isolates from Colombia and Venezuela.

Moncayo

Medina

Kalvatchev

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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“…Furthermore, there are several logical bases for choosing specific mutations to engineer into the VEE genome [12], and an array of animal models [9,13,14] with which to test the biological consequences (e.g. attenuation, immunogenicity) of such mutations.…”

Section: Subject Termsmentioning

confidence: 99%

“…Parent V3000 virus was passaged twice in BHK cells after collection from transfection supernatant fluids. Cleavage-signal deletion with resuscitating mutation Cleavage-signal mutation with resuscitating mutation Cleavage-signal deletion with resuscitating mutation Cleavage-signal mutation with resuscitating mutation a The genome of V3000 differs from the Trinidad Donkey strain sequence [12] by a silent E2 170 change and an adventitious change at E2 239 (He for Asn). He at E2 239 was also identified in the sequence of a clonal isolate of the virulent Trinidad donkey strain of VEE [19] and therefore is not considered to be a candidate attenuating mutation.…”

Section: Clones Viruses and Cellsmentioning

confidence: 99%

Genetically engineered, live attenuated vaccines for Venezuelan equine encephalitis: testing in animal models

Pratt

Davis

Johnston

et al. 2003

Vaccine

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“…Complete sequences of 1,677 nucleotides covering the P62 gene were determined directly from RT-PCR amplicons and compared to homologous VEE complex sequences found in the GenBank library. 12,17,[25][26][27][28] The Miranda isolates exhibited a minimum nucleotide sequence divergence of 8% (3% divergence of deduced amino acid sequences) versus all other VEE complex viruses sequenced previously. Phylogenetic analyses using all methods generated trees with nearly identical topologies, with the exception of a minor difference in groupings within the subtype IAB clade in two equally parsimonious trees.…”

Section: Virus Isolations and Rodent Serologymentioning

confidence: 99%

Ecological studies of enzootic Venezuelan equine encephalitis in north-central Venezuela, 1997-1998.

Salas¹,

García

Liria

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. From 1997From -1998, we investigated the possible continuous circulation of epizootic Venezuelan equine encephalitis (VEE) virus suggested by a 1983 subtype IC interepizootic mosquito isolate made in Panaquire, Miranda State, Venezuela. The study area was originally covered by lowland tropical rainforest but has been converted into cacao plantations. Sentinel hamsters, small mammal trapping, mosquito collections, and human serosurveys were used to detect active or recent virus circulation. Six strains of subtype ID VEE virus were isolated from hamsters that displayed no apparent disease. Four other arboviruses belonging to group A (Togaviridae: Alphavirus), two Bunyamwera group (Bunyaviridae), and three Gamboa group (Bunyaviridae) arboviruses were also isolated from hamsters, as well as 8 unidentified viruses. Venezuelan equine encephalitis-specific antibodies were detected in 5 small mammal species: Proechimys guairae, Marmosa spp., and Didelphis marsupialis. Mosquito collections comprised of 38 different species, including 8 members of the subgenus Culex (Melanoconion), did not yield any virus isolates. Sera from 195 humans, either workers in the cacao plantation or nearby residents, were all negative for VEE virus antibodies. Sequences of 1,677 nucleotides from the P62 gene of 2 virus isolates indicated that they represent a subtype ID lineage that is distinct from all others characterized previously, and are unrelated to epizootic VEE emergence.

show abstract

The full-length nucleotide sequences of the virulent Trinidad donkey strain of Venezuelan equine encephalitis virus and its attenuated vaccine derivative, strain TC-83

Cited by 158 publications

References 43 publications

Genetic diversity and relationships among Venezuelan equine encephalitis virus field isolates from Colombia and Venezuela.

Genetic diversity and relationships among Venezuelan equine encephalitis virus field isolates from Colombia and Venezuela.

Genetically engineered, live attenuated vaccines for Venezuelan equine encephalitis: testing in animal models

Ecological studies of enzootic Venezuelan equine encephalitis in north-central Venezuela, 1997-1998.

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