The functional maturation of the Sertoli cell and Leydig cell in the mammalian testis

Waites, G. M. H.; Speight, Alison; Jenkins, N.

doi:10.1530/jrf.0.0750317

Cited by 30 publications

(17 citation statements)

References 58 publications

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“…Follicle stimulating hormone (FSH) receptors on SCs increases markedly before birth (Steinberger et al, 1974;Hodgson et al, 1983;Warren et al, 1984) and peaks BPND 18 with a decline until adult levels met BPND 40-50 (Waites et al, 1985).…”

Section: Sertoli Cellsmentioning

confidence: 99%

Development and maturation of the male reproductive system

Marty

Chapin

Parks

et al. 2003

Birth Defects Research Pt B

128

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Section: Sertoli Cellsmentioning

confidence: 99%

Development and maturation of the male reproductive system

Marty

Chapin

Parks

et al. 2003

Birth Defects Research Pt B

128

View full text Add to dashboard Cite

“…In the testis the diploid spermatocyte must pass between adjacent Sertoli cells to complete meiosis and gain access to the tubule lumen (Waites, Speight & Jenkins, 1985). In the rat testis, PA is concentrated within regions of the seminiferous tubule where spermatocyte migration occurs (Vikho, Toppari, Saksela et al 1986), and Sertoli cell-enriched cultures are induced to secrete PA by follicle-stimulating hor¬ mone (FSH) stimulation (Fritz & Tung, 1986).…”

Section: Introductionmentioning

confidence: 99%

“…However, the uPA-like protease (40-45 kDa) of the rat testis does not migrate as authentic human uPA (54 kDa) or its fragment (33 kDa) on gels (Hettle, Waller & Fritz, 1986;Vikho et al 1986). It is also not clear whether the rat model can be extrapolated to the protease composition in species undergoing true puberty, such as primates and ungulates (Waites et al 1985). In view of the different structural and func¬ tional properties displayed by the two forms of PA, it is important to determine by a number of different techniques which PA variant is present in the Sertoli cell, and to define the regulatory mechanisms which control its synthesis.…”

Section: Introductionmentioning

confidence: 99%

Only tissue-type plasminogen activator is secreted by immature bovine Sertoli cell-enriched cultures

Coombs

Ellison²,

Woods³

et al. 1988

Journal of Endocrinology

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Two molecular variants of plasminogen activator (PA): urokinase (uPA) and tissue-type plasminogen activator (tPA), have been reported to be synthesized in the rat testis. Data obtained in this study using monospecific antibodies raised against uPA and tPA in immunoblotting and bioimmunoassay protocols consistently demonstrate that only tPA (and not uPA) is synthesized by bovine Sertoli cell-enriched cultures, and is induced by bovine FSH. Zymographic analysis of conditioned medium on gels containing plasminogen and casein showed a dominant PA proteolytic band (72 kDa) which co-migrated with human tPA. A proteolytic band (43 kDa), which was also secreted by FSH-stimulated cells, was not present when protection was afforded from auto-proteolysis by aprotinin, and was therefore concluded to be a proteolytic fragment of tPA, and not uPA.

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“…The transformed cells did not exhibit contact inhibition, as the Swiss 3T3 cells did, when they attained confluence in culture. This difference may be somewhat analogous to the situation with testicular cells, among which somatic cells (Sertoli cells and Leydig cells) only proliferate during early postnatal life whereas germ cell proliferation continues throughout adult life [30]. Thus the differences in the responses of the two cell lines of fibroblasts may be related to the proliferative nature of the cell lines.…”

Section: Discussionmentioning

confidence: 83%

Ontogeny of Rat Testicular Mitogenic Activity Studied with Transformed and Nontransformed Mouse 3T3 Fibroblasts

Searle

Pogach²,

Nathan³

et al. 1990

Archives of Andrology

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The ontogeny of the testicular mitogenic activity of Sprague Dawley rats was evaluated by stimulation in vitro of [3H]thymidine uptake and cell number in Swiss 3T3 and transformed NIH 3T3 fibroblasts. Addition of various amounts of testicular cytosolic protein resulted in a dose-dependent increase in cell number in both cell lines. The [3H]thymidine responses to testicular cytosolic proteins was also dose dependent but in a nonparametric manner. In addition, the responses of 3T3 cells to testicular cytosol was apparently dependent on the developmental stage of the testis, the time of stimulation, and the proliferative nature of the fibroblast cell line. These results suggest the presence of multiple forms of mitogenic substance in testicular cytosol and imply that the relative amounts of these substances may depend on the age of the donor animal. These substances may be important in the proliferation or differentiation of cell types at various stages of testicular development.

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The functional maturation of the Sertoli cell and Leydig cell in the mammalian testis

Cited by 30 publications

References 58 publications

Development and maturation of the male reproductive system

Development and maturation of the male reproductive system

Only tissue-type plasminogen activator is secreted by immature bovine Sertoli cell-enriched cultures

Ontogeny of Rat Testicular Mitogenic Activity Studied with Transformed and Nontransformed Mouse 3T3 Fibroblasts

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