The GanB Gα-Protein Negatively Regulates Asexual Sporulation and Plays a Positive Role in Conidial Germination in Aspergillus nidulansSequence data from this article have been deposited with the EMBL/GenBank Data Libraries under accession no. AF198116.

Chang, Mi-Hee; Chae, Keon‐Sang; Han, Dong-Min; Jahng, Kwang-Yeop

doi:10.1534/genetics.103.025379

Cited by 95 publications

(123 citation statements)

References 52 publications

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“…FluG activates asexual development by removing repressive effects imposed by multiple negative regulators (Seo et al 2003), resulting in activation of a key transcription factor BrlA (see Adams et al 1998). Genetic data suggest that GanB (Chang et al 2004) and SfaD (Yu et al 1996;Rosén et al 1999) function in negative regulation of conidiation under submerged culture conditions. It is further speculated that FadA and SfaDTGpgA may also function in signaling for sexual fruiting body development.…”

Section: Discussionmentioning

confidence: 99%

“…(dominant negative allele), DsfaD, and the absence of GanB (another Ga) functions suggest that GanB and SfaD play a role in negative regulation of conidiation under submerged culture conditions (Rosén et al 1999;Chang et al 2004;Han et al 2004b).…”

Section: G203rmentioning

confidence: 99%

“…In addition, a recent study showed that GNG-1 (Gg) is necessary for normal female fertility in N. crassa (Krystofova and Borkovich 2005). Deletion of ganA or ganB encoding additional Ga-subunits in A. nidulans is found to cause no effects in sexual development (Chang et al 2004;K.-Y. Jahng, personal communication).…”

Section: G203rmentioning

confidence: 99%

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Multiple Roles of a Heterotrimeric G-Protein γ-Subunit in Governing Growth and Development of Aspergillus nidulans

Seo¹,

Han²,

2005

Genetics

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Vegetative growth signaling in the filamentous fungus Aspergillus nidulans is primarily mediated by the heterotrimeric G-protein composed of FadA (Ga), SfaD (Gb), and a presumed Gg. Analysis of the A. nidulans genome identified a single gene named gpgA encoding a putative Gg-subunit. The predicted GpgA protein consists of 90 amino acids showing 72% similarity with yeast Ste18p. Deletion (D) of gpgA resulted in restricted vegetative growth and lowered asexual sporulation. Moreover, similar to the DsfaD mutant, the DgpgA mutant was unable to produce sexual fruiting bodies (cleistothecia) in self-fertilization and was severely impaired with cleistothecial development in outcross, indicating that both SfaD and GpgA are required for fruiting body formation. Developmental and morphological defects caused by deletion of flbA encoding an RGS protein negatively controlling FadA-mediated vegetative growth signaling were suppressed by DgpgA, indicating that GpgA functions in FadA-SfaD-mediated vegetative growth signaling. However, deletion of gpgA could not bypass the need for the early developmental activator FluG in asexual sporulation, suggesting that GpgA functions in a separate signaling pathway. We propose that GpgA is the only A. nidulans Gg-subunit and is required for normal vegetative growth as well as proper asexual and sexual developmental progression.

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Section: Discussionmentioning

confidence: 99%

Section: G203rmentioning

confidence: 99%

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Multiple Roles of a Heterotrimeric G-Protein γ-Subunit in Governing Growth and Development of Aspergillus nidulans

Seo¹,

Han²,

2005

Genetics

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“…2). The gasC orthologues, rasA, and cflA are also important for conidial germination in the taxonomically closest model fungus Aspergillus nidulans, in Botrytis cinerea, and in a variety of other fungal species (3,(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21).…”

mentioning

confidence: 99%

Morphogenetic Circuitry Regulating Growth and Development in the Dimorphic Pathogen Penicillium marneffei

Boyce

Andrianopoulos

2013

Eukaryot Cell

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Penicillium marneffei is an emerging human-pathogenic fungus endemic to Southeast Asia. Like a number of other fungal pathogens, P. marneffei exhibits temperature-dependent dimorphic growth and grows in two distinct cellular morphologies, hyphae at 25°C and yeast cells at 37°C. Hyphae can differentiate to produce the infectious agents, asexual spores (conidia), which are inhaled into the host lung, where they are phagocytosed by pulmonary alveolar macrophages. Within macrophages, conidia germinate into unicellular yeast cells, which divide by fission. This minireview focuses on the current understanding of the genes required for the morphogenetic control of conidial germination, hyphal growth, asexual development, and yeast morphogenesis in P. marneffei. MORPHOGENESIS DURING THE GROWTH AND DEVELOPMENT OF PENICILLIUM MARNEFFEI

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“…Genomic sequencing has revealed that most filamentous ascomycetes have three Ga-subunits (Liu and Dean 1997;Chang et al 2004;Kays and Borkovich 2004). Among them, Ga-subunits of the fungal group I share specific sequence similarities with the mammalian G i subunits, while the subunits of group III have been assigned as G s subunits on the basis of their functionality in stimulating adenylyl cyclases like their mammalian counterparts.…”

mentioning

confidence: 99%

Three α-Subunits of Heterotrimeric G Proteins and an Adenylyl Cyclase Have Distinct Roles in Fruiting Body Development in the Homothallic Fungus Sordaria macrospora

et al. 2008

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Sordaria macrospora, a self-fertile filamentous ascomycete, carries genes encoding three different a-subunits of heterotrimeric G proteins (gsa, G protein Sordaria alpha subunit). We generated knockout strains for all three gsa genes (Dgsa1, Dgsa2, and Dgsa3) as well as all combinations of double mutants. Phenotypic analysis of single and double mutants showed that the genes for Ga-subunits have distinct roles in the sexual life cycle. While single mutants show some reduction of fertility, double mutants Dgsa1Dgsa2 and Dgsa1Dgsa3 are completely sterile. To test whether the pheromone receptors PRE1 and PRE2 mediate signaling via distinct Ga-subunits, two recently generated Dpre strains were crossed with all Dgsa strains. Analyses of the corresponding double mutants revealed that compared to GSA2, GSA1 is a more predominant regulator of a signal transduction cascade downstream of the pheromone receptors and that GSA3 is involved in another signaling pathway that also contributes to fruiting body development and fertility. We further isolated the gene encoding adenylyl cyclase (AC) (sac1) for construction of a knockout strain. Analyses of the three DgsaDsac1 double mutants and one Dgsa2Dgsa3Dsac1 triple mutant indicate that SAC1 acts downstream of GSA3, parallel to a GSA1-GSA2-mediated signaling pathway. In addition, the function of STE12 and PRO41, two presumptive signaling components, was investigated in diverse double mutants lacking those developmental genes in combination with the gsa genes. This analysis was further completed by expression studies of the ste12 and pro41 transcripts in wild-type and mutant strains. From the sum of all our data, we propose a model for how different Ga-subunits interact with pheromone receptors, adenylyl cyclase, and STE12 and thus cooperatively regulate sexual development in S. macrospora.

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The GanB Gα-Protein Negatively Regulates Asexual Sporulation and Plays a Positive Role in Conidial Germination in Aspergillus nidulansSequence data from this article have been deposited with the EMBL/GenBank Data Libraries under accession no. AF198116.

Cited by 95 publications

References 52 publications

Multiple Roles of a Heterotrimeric G-Protein γ-Subunit in Governing Growth and Development of Aspergillus nidulans

Multiple Roles of a Heterotrimeric G-Protein γ-Subunit in Governing Growth and Development of Aspergillus nidulans

Morphogenetic Circuitry Regulating Growth and Development in the Dimorphic Pathogen Penicillium marneffei

Three α-Subunits of Heterotrimeric G Proteins and an Adenylyl Cyclase Have Distinct Roles in Fruiting Body Development in the Homothallic Fungus Sordaria macrospora

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