The Gcn5 complexes in Drosophila as a model for metazoa

Torres-Zelada, Eliana F.; Weake, Vikki M.

doi:10.1016/j.bbagrm.2020.194610

Cited by 12 publications

(11 citation statements)

References 137 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ada2b interacts with the HAT (histone acetyltransferase) module containing GCN5 and Ada3. The HAT module further associates with several other proteins to form large protein complexes (such as the SAGA complex) [ 30 , 31 , 42 ]. The Ada2b-containing complex is reported to be involved in the epigenetic regulation of gene expression.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome features of innate immune memory in Drosophila

Fuse

Okamori²,

Okaji³

et al. 2022

PLoS Genet

View full text Add to dashboard Cite

Immune memory is the ability of organisms to elicit potentiated immune responses at secondary infection. Current studies have revealed that similar to adaptive immunity, innate immunity exhibits memory characteristics (called "innate immune memory"). Although epigenetic reprogramming plays an important role in innate immune memory, the underlying mechanisms have not been elucidated, especially at the individual level. Here, we established experimental systems for detecting innate immune memory in Drosophila melanogaster. Training infection with low-pathogenic bacteria enhanced the survival rate of the flies at subsequent challenge infection with high-pathogenic bacteria. Among low-pathogenic bacteria, Micrococcus luteus (Ml) and Salmonella typhimurium (St) exerted apparent training effects in the fly but exhibited different mechanisms of action. Ml exerted training effects even after its clearance from flies, while live St persisted in the flies for a prolonged duration. RNA sequencing (RNA-Seq) analysis revealed that Ml training enhanced the expression of the immune-related genes under the challenge condition but not under the non-challenge condition. In contrast, St training upregulated the expression of the immune-related genes independent of challenge. These results suggest that training effects with Ml and St are due to memory and persistence of immune responses, respectively. Furthermore, we searched for the gene involved in immune memory, and identified a candidate gene, Ada2b, which encodes a component of the histone modification complex. The Ada2b mutant suppressed Ml training effects on survival and disrupted the expression of some genes under the training + challenge condition. These results suggest that the gene expression regulated by Ada2b may contribute to innate immune memory in Drosophila.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptome features of innate immune memory in Drosophila

Fuse

Okamori²,

Okaji³

et al. 2022

PLoS Genet

View full text Add to dashboard Cite

show abstract

“…Gene expression profiling of Ada2b mutants has revealed widespread disruption of gene expression that was historically attributed to the loss of SAGA activity ( Torres-Zelada and Weake, 2020 ). However, the recent finding that alternative splicing of Ada2b can generate new diversity in Gcn5 complexes raises the question of whether these complexes have overlapping or distinct roles in regulating gene expression.…”

Section: Resultsmentioning

confidence: 99%

Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos

Torres-Zelada

George

Blum

et al. 2022

Journal of Cell Science

Self Cite

View full text Add to dashboard Cite

The histone acetyltransferase Gcn5 is critical for gene expression and development. In Drosophila, Gcn5 is part of four complexes (SAGA, ATAC, CHAT, and ADA) that are essential for fly viability and have key roles in regulating gene expression. Here, we show that while the SAGA, ADA, and CHAT complexes play redundant roles in embryonic gene expression, the insect-specific CHAT complex uniquely regulates expression of a subset of developmental genes. We also identify a substantial decrease in histone acetylation in chiffon mutant embryos that exceeds that observed in ada2b, suggesting broader roles for Chiffon in regulating histone acetylation outside of the Gcn5 complexes. The chiffon gene encodes two independent polypeptides that nucleate formation of either the CHAT or Dbf4-dependent kinase (DDK) complexes. DDK includes the cell cycle kinase Cdc7, which is necessary for maternally-driven DNA replication in the embryo. We identify a temporal switch between the expression of these chiffon gene products during a short window during the early nuclear cycles in embryos that correlates with the onset of zygotic genome activation, suggesting a potential role for CHAT in this process.

show abstract

“…Instead, uncharacterized translational mechanisms are thought to underly the switch between Chiffon‐A and Chiffon‐B expression (Torres‐Zelada et al, 2022). Chiffon interacts with the histone acetyltransferase Gcn5 and three partner proteins, Ada2b‐PA, Sgf29 and Ada3 to nucleate formation of the CHAT and a schematic illustrating the subunit composition of the Drosophila DDK and CHAT complexes are shown in Figure 1c (Torres‐Zelada et al, 2019; Torres‐Zelada & Weake, 2021).…”

Section: Resultsmentioning

confidence: 99%

“…Gcn5/KAT2A was the first nuclear histone acetyltransferase to be identified, and functions as part of multi‐subunit complexes that have key roles in gene expression and are widely conserved in yeast, Drosophila , plants and mammals (Torres‐Zelada & Weake, 2021). Recently, gene expression profiling of Drosophila embryos revealed that CHAT has an important role in regulating the expression of developmental genes, albeit partially redundant with other Gcn5‐containing complexes (Torres‐Zelada et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

The interaction between the Dbf4 ortholog Chiffon and Gcn5 is conserved in Dipteran insect species

George

Blum

Torres-Zelada

et al. 2022

Insect Molecular Biology

View full text Add to dashboard Cite

Chiffon is the sole Drosophila ortholog of Dbf4, the regulatory subunit for the cell‐cycle kinase Cdc7 that initiates DNA replication. In Drosophila, the chiffon gene encodes two polypeptides with independent activities. Chiffon‐A contains the conserved Dbf4 motifs and interacts with Cdc7 to form the Dbf4‐dependent Kinase (DDK) complex, which is essential for a specialized form of DNA replication. In contrast, Chiffon‐B binds the histone acetyltransferase Gcn5 to form the Chiffon histone acetyltransferase (CHAT) complex, which is necessary for histone H3 acetylation and viability. Previous studies have shown that the Chiffon‐B region is only present within insects. However, it was unclear how widely the interaction between Chiffon‐B and Gcn5 was conserved among insect species. To examine this, we performed yeast two‐hybrid assays using Chiffon‐B and Gcn5 from a variety of insect species and found that Chiffon‐B and Gcn5 interact in Diptera species such as Australian sheep blowfly and yellow fever mosquito. Protein domain analysis identified that Chiffon‐B has features of acidic transcriptional activators such as Gal4 or VP16. We propose that the CHAT complex plays a critical role in a biological process that is unique to Dipterans and could therefore be a potential target for pest control strategies.

show abstract

The Gcn5 complexes in Drosophila as a model for metazoa

Cited by 12 publications

References 137 publications

Transcriptome features of innate immune memory in Drosophila

Transcriptome features of innate immune memory in Drosophila

Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos

The interaction between the Dbf4 ortholog Chiffon and Gcn5 is conserved in Dipteran insect species

Contact Info

Product

Resources

About