The Gel Test

Malyska, Harry; Weiland, Deborah

doi:10.1093/labmed/25.2.81

Cited by 26 publications

(23 citation statements)

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“…24 The procedures used in humans are standardized and provide clear and stable reactions that improve result interpretation. The gel test cards can be saved for up to 24 hours, 25 and photocopies are readily achievable. This technology was recently applied for canine DEA 1.1 typing, by using a monoclonal antibody, and initial comparative studies concluded that it is a reliable and rapid laboratory method for canine DEA 1.1 blood typing.…”

Section: Discussionmentioning

confidence: 99%

Canine Dal Blood Type: A Red Cell Antigen Lacking in Some Dalmatians

Blais

Berman

Oakley

et al. 2007

Veterinary Internal Medicne

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Background: Based upon alloantibodies produced after sensitizing dogs with transfused blood, more than a dozen blood group systems have been recognized thus far, and some have been classified as dog erythrocyte antigens (DEA).Hypothesis: A new canine red cell antigen was suspected, based on the development of specific alloantibodies in a Dalmatian previously sensitized by blood transfusions.Animals: Twenty-six Dalmatians (including 1 Dalmatian in need of blood compatibility studies); 55 canine blood donors. Methods: Serologic tests, including blood typing, crossmatching, and direct Coombs' test were performed by standard tube techniques and a novel gel column technology adapted from human blood banking.Results: By day 40 after transfusion of an anemic Dalmatian, all major crossmatch tests to 55 non-Dalmatian dogs were incompatible. The 2 initial donors, who were compatible before transfusion, were also now incompatible, suggesting the development of an alloantibody to a common red cell antigen. No siblings were available, but 4 of 25 unrelated Dalmatians were crossmatch compatible, suggesting that they were missing the same red cell antigen. The patient was blood typed DEA 1.1, 3, 4, and 5 positive, but DEA 7 negative. Further blood typing and crossmatching results did not support an association to any of these known blood types. The alloantibodies produced were determined to be of the immunoglobulin G class.Conclusions and Clinical Importance: Based upon the identification of an acquired alloantibody in a Dalmatian, a presumably new common blood type named Dal was identified. Dalmatians lacking the Dal antigen are likely at risk of delayed and acute hemolytic transfusion reactions.

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Section: Discussionmentioning

confidence: 99%

Canine Dal Blood Type: A Red Cell Antigen Lacking in Some Dalmatians

Blais

Berman

Oakley

et al. 2007

Veterinary Internal Medicne

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“…The basic principle of the gel test is that instead of a test tube, the serum and cell reaction takes place in a microtube. Six of such microtubes are embedded in a plastic card to allow ease of handling, testing, reading and disposal [8].…”

Section: Discussionmentioning

confidence: 99%

Comparative Study of Blood Cross Matching Using Conventional Tube and Gel Method

Swarup¹,

Dhot²,

Kotwal

et al. 2008

Medical Journal Armed Forces India

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Background: Conventionally tube method is used for compatibility and cross matching in transfusion medicine. Methods: A comparative study was carried out to evaluate the efficacy of conventional tube and gel technique. Result: Compatibility testing was performed on 1000 blood samples by conventional test tube method and DiaMed gel method. The results were analysed. Conclusion: The gel method was found to be a rapid and reliable procedure without controls. There was no requirement of wash phase in indirect antiglobulin test and sensitivity and specificity was comparable to spin tube method. We conclude that this technique is a better substitute for spin tube method. MJAFI 2008; 64 : 129-130

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“…Variation in results between laboratories is not uncommon, as some laboratories still use enhancing media so as not to miss low titer samples (Moise, 2002). Malyska & Weiland (1994) pointed out that testing by the gel microcolumn assay requires only small volumes of blood sample, is relatively stable at room temperature and reaction endpoints are standardized, making this method theoretically ideal for prenatal antibody titration studies. Biosafety is also enhanced in the gel system, by the elimination of glass tubes (Lapierre et al, 1990;Novaretti et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Comparison of conventional tube test with diamed gel microcolumn assay for anti-D titration

Novaretti

Jens

Pagliarini

et al. 2003

Clinical &Amp; Laboratory Haematology

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Anti-D titration is the first step in the evaluation of the RhD-sensitized patient. Traditionally, anti-D titration has been performed by tube agglutination. Gel microcolumn assay is a method that has gained widespread usage throughout the world, mainly for ABO/Rh typing, unexpected antibody screening and direct antiglobulin tests. As gel assay has become widely used as a routine method to detect red blood cell alloantibodies, a critical anti-D titer needs to be established. Seventy-nine known blood samples with anti-D (titers 1-32) were titrated simultaneously by the conventional tube test and the gel microcolumn assay. Red blood cells (R0r phenotype) were used, with a final concentration of 3% for tube and 0.8% for gel. Serial twofold dilutions (2-2.048) were prepared for each technique, followed by reading in antiglobulin phase. Anti-D titration in the gel microcolumn assay showed significantly higher titers (mean 3.4-fold) than the conventional tube test in all samples studied. Based on these data, it was not possible to determine a critical titer for anti-D titration by the gel microcolumn assay.

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The Gel Test

Cited by 26 publications

References 0 publications

Canine Dal Blood Type: A Red Cell Antigen Lacking in Some Dalmatians

Canine Dal Blood Type: A Red Cell Antigen Lacking in Some Dalmatians

Comparative Study of Blood Cross Matching Using Conventional Tube and Gel Method

Comparison of conventional tube test with diamed gel microcolumn assay for anti-D titration

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