The gene expression profile of Bombyx mori silkgland

Tang, Jie; Li, Weifang; Zhang, Xuan; Zhou, Cong‐Zhao

doi:10.1016/j.gene.2007.04.014

Cited by 9 publications

(7 citation statements)

References 19 publications

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“…The silk gland is a specialized organ to synthesize and secrete silk proteins. Silk fibroin, a major component of silk protein, is composed of a complex ([fibroin heavy chain ( Fib‐H ) ‐ fibroin light chain ( Fib‐L )] 6 ‐ P25 ) (Inoue et al ., ; Zhou et al ., ; Tang et al ., ). Our previous study showed that there is no difference in expression levels of the genes encoding silk proteins between domestic and wild silkworms (Fang et al ., ).…”

Section: Discussionmentioning

confidence: 97%

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Identification and comparison of long non‐coding RNAs in the silk gland between domestic and wild silkworms

et al. 2017

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Under long-term artificial selection, the domestic silkworm (Bombyx mori) has increased its silk yield tremendously in comparison with its wild progenitor, Bombyx mandarina. However, the molecular mechanism of silk yield increase is still unknown. Comparative analysis of long non-coding RNAs (lncRNAs) may provide some insights into understanding this phenotypic variation. In this study, using RNA sequencing technology data of silk gland in domestic and wild silkworms, we identified 599 lncRNAs in the silk gland of the silkworm. Compared with protein-coding genes, the silk gland lncRNA genes tend to have fewer exon numbers, shorter transcript length and lower GC-content. Moreover, we found that three lncRNA genes are significantly and differentially expressed between domestic and wild silkworms. The potential targets of two differentially expressed lncRNAs (DELs) (dw4sg_0040 and dw4sg_0483) and the expression-correlated genes with the two DELs are mainly enriched in the related processes of silk protein translation. This implies that these DELs may affect the phenotypic variation in silk yield between the domestic and wild silkworms through the post-transcriptional regulation of silk protein.

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Section: Discussionmentioning

confidence: 97%

“…Silk yield is an important economic trait and a domestication trait. However, little is known about the molecular mechanism of the silk yield variation between domestic and wild silkworms (Tang et al ., ; Li et al ., ; Li et al ., ; Wu et al ., ). The silk gland is a specialized organ to synthesize and secrete silk proteins.…”

Section: Discussionmentioning

confidence: 99%

Identification and comparison of long non‐coding RNAs in the silk gland between domestic and wild silkworms

et al. 2017

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“…The domestic silkworm has long been studied as a model lepidopteran insect and many studies have examined gene expression in the silk glands of this species (Tang et al ., ; Royer et al ., ). Although eri and domestic silkworms are closely related phylogenetically (Regier et al ., ), the properties of their silks are very different.…”

Section: Resultsmentioning

confidence: 99%

“…‡ Categorized as A/MSG upregulated genes in the Bombyx (Xia et al, 2007). (Tang et al, 2007;Royer et al, 2011). Although eri and domestic silkworms are closely related phylogenetically (Regier et al, 2009), the properties of their silks are very different.…”

Section: Comparison Of Gene Expression Between Eri and Domestic Silkw...mentioning

confidence: 99%

Gene expression analysis in the larval silk gland of the eri silkworm Samia ricini

et al. 2015

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Insects produce silk for a range of purposes. In the Lepidoptera, silk is utilized as a material for cocoon production and serves to protect larvae from adverse environmental conditions or predators. Species in the Saturniidae family produce an especially wide variety of cocoons, for example, large, golden colored cocoons and those with many small holes. Although gene expression in the silk gland of the domestic silkworm (Bombyx mori L.) has been extensively studied, considerably fewer investigations have focused on members of the saturniid family. Here, we established expression sequence tags from the silk gland of the eri silkworm (Samia ricini), a saturniid species, and used these to analyze gene expression. Although we identified the fibroin heavy chain gene in the established library, genes for other major silk proteins, such as fibroin light chain and fibrohexamerin, were absent. This finding is consistent with previous reports that these latter proteins are lacking in saturniid silk. Recently, a series of fibrohexamerin-like genes were identified in the Bombyx genome. We used this information to conduct a detailed analysis of the library established here. This analysis identified putative homologues of these genes. We also found several genes encoding small silk protein molecules that are also present in the silk of other Lepidoptera. Gene expression patterns were compared between eri and domestic silkworm, and both conserved and nonconserved expression patterns were identified for the tested genes. Such differential gene expression might be one of the major causes of the differences in silk properties between these species. We believe that our study can be of value as a basic catalogue for silk gland gene expression, which will yield to the further understanding of silk evolution.

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“…To date, the autophagy-related gene ATG8 of many insects, such as the silkworm, D. melanogaster, Helicoverpa armigera, Spodoptera litura and Trichoplusia ni, has been cloned (Tang et al, 2007;Owa et al, 2008;Zhang et al, 2009;Khoa & Takeda, 2012). The ATG8 protein combines with PE (i.e.…”

Section: Discussionmentioning

confidence: 99%

Starvation‐induced autophagy in Spodoptera frugiperda Sf9 ovarian cells

et al. 2019

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Autophagy controls insect development and can be targeted for pest control in agriculture. In the present study, starvation‐induced autophagy is investigated in the insect species Spodoptera frugiperda. Bioinformatics analysis and a search of the EST database (http://bioweb.ensam.inra.fr/spodobase) identifies a putative ATG8 gene of S. frugiperda. To generate a biomarker of autophagosome, the DNA sequence encoding the open reading frame of this gene is amplified and cloned into a pIEX‐4‐mCherry‐EGFP‐SfATG8 recombinant vector. Sf9 cells are then transfected with this expression vector and starved in phosphate‐buffered saline solution for 4 h to induce autophagy, which is examined by LysoTracker staining (Life Technologies, Grand Island, New York), western blotting and fluorescence microscopy. The results obtained show that starvation stimulates lipidation of SfATG8‐PE and the formation of autophagosomes, providing a foundation for further research with respect to autophagy in insects.

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The gene expression profile of Bombyx mori silkgland

Cited by 9 publications

References 19 publications

Identification and comparison of long non‐coding RNAs in the silk gland between domestic and wild silkworms

Identification and comparison of long non‐coding RNAs in the silk gland between domestic and wild silkworms

Gene expression analysis in the larval silk gland of the eri silkworm Samia ricini

Starvation‐induced autophagy in Spodoptera frugiperda Sf9 ovarian cells

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