The Gene Pool of Miscanthus Species and Its Improvement

Sacks, Erik J.; Juvik, John A.; Qi, Lin; Stewart, J. Ryan; Yamada, Toshihiko

doi:10.1007/978-1-4419-5947-8_4

Cited by 72 publications

(140 citation statements)

References 53 publications

Supporting

Mentioning

136

Contrasting

Unclassified

Order By: Relevance

“…Miscanthus 3 giganteus, a triploid sterile hybrid, is an appealing perennial bioenergy crop with high biomass production and low environmental impact. However, only a few clones exist, and biomass potential varies in different climates, emphasizing the need for region-specific varieties (Sacks et al, 2013;Arnoult and Brancourt-Hulmel, 2015). Sugarcane is valued for food, ethanol, and biomass production, and there is a need for new varieties with high sugar and biomass yield.…”

Section: Vif: a Powerful Tool In A Spectrum Of Recalcitrant Plants?mentioning

confidence: 99%

Virus-Induced Flowering: An Application of Reproductive Biology to Benefit Plant Research and Breeding

et al. 2016

View full text Add to dashboard Cite

Section: Vif: a Powerful Tool In A Spectrum Of Recalcitrant Plants?mentioning

confidence: 99%

Virus-Induced Flowering: An Application of Reproductive Biology to Benefit Plant Research and Breeding

et al. 2016

View full text Add to dashboard Cite

“…According to Meyer and Tchida (1999), since 1980 over 50 Miscanthus selections have been introduced, mainly from the United States and Germany. Currently, in the USA nursery market more than 85 M. sinensis cultivars are available (Sacks et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…varies from diploid to hexaploid. M. sacchariflorus accessions native to China are mainly diploids, while accessions native to Japan are polyploids, mostly tetraploids (Moon et al, 2013;Sacks et al, 2013). M. sinensis is typically diploid, but natural and artificial polyploids are available.…”

Section: Introductionmentioning

confidence: 99%

“…M. sinensis is typically diploid, but natural and artificial polyploids are available. One of such examples is a triploid cultivar known as 'Goliath' (CliftonBrown et al, 2008;Sacks et al, 2013). According to Purdy et al (2013), it is an intraspecific hybrid of M. sinensis, which was selected by Ernst Pagels as a vigorous seedling from crossing of unknown parents.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Miscanthus: Inter- and Intraspecific Genome Size Variation Among M. × Giganteus, M. Sinensis, M. Sacchariflorus Accessions

Cichorz¹,

Goska²,

Rewers³

2015

Acta Biologica Cracoviensia S. Botanica

View full text Add to dashboard Cite

Since M. sinensis Anderss., M. sacchariflorus (Maxim.) Hack. and M. ×giganteus J.M.Greef & Deuter ex Hodk. and Renvoize have considerably the highest potential for biomass production among Miscanthus Anderss. species, there is an urgent need to broaden the knowledge about cytological characteristics required for their improvement. In this study our objectives were to assess the genome size variation among eighteen Miscanthus accessions, as well as estimation of the monoploid genome size (2C and Cx) of the M. sinensis cultivars, which have not been analyzed yet. The characterization of three Miscanthus species was performed with the use of flow cytometry and analysis of the stomatal length. The triploid (2n = 3x = 57) M. sinensis 'Goliath' and M. ×gigan-teus clones possessed the highest 2C DNA content (8.34 pg and 7.43 pg, respectively). The intermediate 2C-values were found in the nuclei of the diploid (2n = 2x = 38) M. sinensis accessions (5.52-5.72 pg), whereas they were the lowest in the diploid (2n = 2x = 38) M. sacchariflorus ecotypes (4.58-4.59 pg). The presented study revealed interspecific variation of nuclear DNA content (P<0.01) and therefore allowed for recognition of particular taxa, inter-and intraspecific hybrids and prediction of potential parental components. Moreover, intraspecific genome size variation (P<0.01) was observed in M. sinensis cultivars at 3.62%. The values of the stomatal size obtained for the triploid M. ×giganteus 'Great Britain' (mean 30.70 μm) or 'Canada' (mean 29.67 μm) and diploid M. sinensis 'Graziella' (mean 29.96 μm) did not differ significantly, therefore this parameter is not recommended for ploidy estimation.K Ke ey yw wo or rd ds s: : Flow cytometry, intraspecific variation, Miscanthus, nuclear DNA content, stomatal size

show abstract

“…Miscanthus species originating from China, the primary center of diversity, are nearly always diploid (2n = 2x = 38) and include M. sinensis and M. sacchariflorus (Sacks et al, 2013, Sun et al, 2010. Species with allopolyploid genome constitutions occur regularly 20 | Chapter 1 1 in the secondary centers of diversity Japan and the Korean peninsula (Sacks et al, 2013). An allotetraploid species (2n = 4x = 76) that is most accurately named M. ogiformis, but is often erroneously referred to as tetraploid M. sacchariflorus, occurs in Japan and has a genome homologous to M. sinensis and a genome homologous to M. sacchariflorus (Sacks et al, 2013).…”

mentioning

confidence: 99%

Targets and tools for optimizing lignocellulosic biomass quality of miscanthus

Weijde¹

View full text Add to dashboard Cite

Evaluation of Miscanthus sinensis biomass quality as feedstock for conversion into different bioenergy products 95 Chapter 5Stability of cell wall composition and saccharification efficiency in Miscanthus across diverse environments 125 Chapter 6Dissecting genetic complexity of miscanthus cell wall composition and biomass quality for biofuels 149 Chapter 7Impact of drought stress on growth and quality of miscanthus for biofuel production 179 Chapter 8 General Discussion 205Acknowledgements 223 About the author 227 List of publications 228Overview of training activities 229 SummaryMiscanthus is a perennial energy grass characterized by a high productivity and resource-use efficiency, making it an ideal biomass feedstock for the production of cellulosic biofuels and a wide range of other biobased value-chains. However, the large-scale commercialization of converting biomass into cellulosic biofuel is hindered by our inability to efficiently deconstruct the plant cell wall. The plant cell wall is a complex and dynamic structure and its components are extensively cross-linked into an unyielding matrix. The production of biofuel depends on the extraction, hydrolysis and fermentation of cell wall polysaccharides, which currently requires energetically and chemically intensive processing operations that negatively affect the economic viability and sustainability of the industry. To address this challenge it is envisioned that the bioenergy feedstocks can be compositionally tailored to increase the accessibility and extractability of cell wall polysaccharides, which would allow a more efficient conversion of biomass into biofuel under milder processing conditions.Extensive phenotypic and genetic diversity in cell wall composition and conversion efficiency was observed in different miscanthus species, including M. sinensis, M. sacchariflorus and interspecific hybrids between these two species. In multiple experiments a twofold increase in the release of fermentable sugars was observed in 'high quality' accessions compared to 'low quality' accessions. The exhaustive characterization of eight highly diverse M. sinensis genotypes revealed novel and distinct breeding targets for different bioenergy conversion routes. The key traits that contributed favourably to the conversion efficiency of biomass into biofuel were a high content of hemicellulosic polysaccharides, extensive cross-linking of hemicellulosic polysaccharides (revealed by a high content of trans-ferulic acids and a high ratio of arabinose-to-xylose), a low lignin content and extensive incorporation of paracoumaric acid into the lignin polymer.Lignin is widely recognized as one of the key factors conveying recalcitrance against enzymatic deconstruction of the cell wall. The incorporation of para-coumaric acid into the lignin polymer is hypothesized to make lignin more easily degradable during alkaline pretreatment, one of the most widely applied processing methods that is used to pretreat biomass prior to enzymatic hydrolysis. Previous studies have shown that red...

show abstract

The Gene Pool of Miscanthus Species and Its Improvement

Cited by 72 publications

References 53 publications

Virus-Induced Flowering: An Application of Reproductive Biology to Benefit Plant Research and Breeding

Virus-Induced Flowering: An Application of Reproductive Biology to Benefit Plant Research and Breeding

Miscanthus: Inter- and Intraspecific Genome Size Variation Among M. × Giganteus, M. Sinensis, M. Sacchariflorus Accessions

Targets and tools for optimizing lignocellulosic biomass quality of miscanthus

Contact Info

Product

Resources

About