We investigate the effect of small diffusion on the principal eigenvalues of linear time-periodic parabolic operators with zero Neumann boundary conditions in one dimensional space. The asymptotic behaviors of the principal eigenvalues, as the diffusion coefficients tend to zero, are established for non-degenerate and degenerate spatial-temporally varying environments. A new finding is the dependence of these asymptotic behaviors on the periodic solutions of a specific ordinary differential equation induced by the drift. The proofs are based upon delicate constructions of super/sub-solutions and the applications of comparison principles.