The generation of oxygen radicals during host plant responses to infection

Sutherland, Mark W.

doi:10.1016/0885-5765(91)90020-i

Cited by 323 publications

(179 citation statements)

References 111 publications

Supporting

Mentioning

168

Contrasting

Unclassified

Order By: Relevance

“…It has become apparent that ROS are an integral response to both biotic and abiotic stress. A large number of reviews have been dedicated to this topic (Apel and Hirt, 2004;Baker and Orlandi, 1995;Barna et al, 2012;Baxter et al, 2013;Foyer and Noctor, 2013;Laloi et al, 2004;Mehdy, 1994;Miller et al, 2008;Mittler, 2002;Mittler et al, 2011;O'Brien et al, 2012a;Sutherland, 1991). In this article, we will briefly review the available methods to detect the formation of ROS in plant tissue.…”

Section: Introductionmentioning

confidence: 99%

Reactive oxygen species and plant resistance to fungal pathogens

et al. 2015

View full text Add to dashboard Cite

Reactive oxygen species (ROS) have been studied for their role in plant development as well as in plant immunity. ROS were consistently observed to accumulate in the plant after the perception of pathogens and microbes and over the years, ROS were postulated to be an integral part of the defence response of the plant. In this article we will focus on recent findings about ROS involved in the interaction of plants with pathogenic fungi. We will describe the ways to detect ROS, their modes of action and their importance in relation to resistance to fungal pathogens. In addition we include some results from works focussing on the fungal interactor and from studies investigating roots during pathogen attack.

show abstract

Section: Introductionmentioning

confidence: 99%

Reactive oxygen species and plant resistance to fungal pathogens

et al. 2015

View full text Add to dashboard Cite

show abstract

“…the release of active oxygen species (AOS) by elicited plant cells. These AOS include superoxide (O[-), hydrogen peroxid,: (H202), and the hydroxyl radical (OH') [1,2]. The signifivance of AOS acting as a signal towards host defense in plant-pathogen interactions has been demonstrated by HaO2.mediated oxidative cross-linking of the plant cell wall during HR [3,4], phytoalexin formation in cultured cells [5][6][7] and induction of cellular protection genes [4].…”

Section: Introductionmentioning

confidence: 99%

Generation of active oxygen in elicited cells of Arabidopsis thaliana is mediated by a NADPH oxidase‐like enzyme

et al. 1996

View full text Add to dashboard Cite

“…The cultures were allowed to grow for an additional hour prior to the addition of oxidants at various concentrations. Thereafter, bacterial growth was monitored hourly for 8 h. Survival experiments were performed by subculturing Xanthomonas overnight cultures into fresh media at 2% inoculum, and this was followed by growth for an additional 3 h to give an OD 600 of approximately 1.0 (mid log) (44). For comparison between different cultures, it was important to use bacteria of the same metabolic state and growth phase since large variations in oxidative stress resistance levels were linked to these parameters (46).…”

Section: Bacteria and Plasmidsmentioning

confidence: 99%

“…Oxidative stress is an important component of normal aerobic life and in bacterial-plant interactions. Increasing production of ROS, including superoxide, H 2 O 2 , and OH⅐, is associated with an active plant defense response and with aerobic respiration (44). ROS are highly toxic to all cellular components, and their rapid detoxification is essential for microbial survival.…”

mentioning

confidence: 99%

Heterologous growth phase- and temperature-dependent expression and H2O2 toxicity protection of a superoxide-inducible monofunctional catalase gene from Xanthomonas oryzae pv. oryzae

et al. 1996

View full text Add to dashboard Cite

Catalase is an important protective enzyme against H 2 O 2 toxicity. Here, we report the characterization of a Xanthomonas oryzae pv. oryzae catalase gene (katX). The gene was localized and its nucleotide sequence was determined. The gene codes for a 77-kDa polypeptide. The deduced katX amino acid sequence shares regions of high identity with other monofunctional catalases in a range of organisms from bacteria to eukaryotes. The transcriptional regulation of katX was atypical of bacterial monofunctional kat genes. Northern (RNA) analysis showed that katX transcription was highly induced by treatments with low concentrations of menadione, a superoxide generator, and methyl methanesulfonate, a mutagen. It was only weakly induced by H 2 O 2 . Unlike in other bacteria, a high level of catalase in Xanthomonas spp. provided protection from the growth-inhibitory and killing effects of H 2 O 2 but not from those of organic peroxides and superoxide generators. Unexpectedly, heterologous expression of katX in Escherichia coli was both growth phase and temperature dependent. Catalase activity in E. coli kat mutants harboring katX on an expression vector was detectable only when the cells entered the stationary phase of growth and at 28؇C. The patterns of transcription regulation, heterologous expression, and physiological function of katX are different from previously studied bacterial kat genes.Catalase is a heme-containing enzyme involved in dismutation of H 2 O 2 to oxygen and water. The enzyme plays an important role in detoxifying H 2 O 2 and minimizing oxidative stress caused by highly reactive oxygen species (ROS, i.e., OH⅐) which arise from H 2 O 2 degradation in the Fenton reaction (13). Mutations in kat have always resulted in increased sensitivity to H 2 O 2 stress, and this is an indication of the important physiological role of the enzyme (10, 13). In many bacteria, there are two types of catalase enzyme, namely a monofunctional catalase and a bifunctional catalase/peroxidase. Each enzyme is encoded by a different gene (e.g., in Escherichia coli, katE and katG code for monofunctional and bifunctional catalases, respectively) (35, 45). Monofunctional catalases share regions of an amino acid sequence that is highly conserved among microbial, plant, and mammalian enzymes (5,26,47). In many bacteria, the two kat genes are regulated differently in terms of growth phase and response to oxidative stress, suggesting that they may have different physiological functions (10,23,29).Xanthomonas oryzae pv. oryzae (Xoo) is the causative agent for the most destructive bacterial disease (the bacterial leaf blight) in rice (37). Oxidative stress is an important component of normal aerobic life and in bacterial-plant interactions. Increasing production of ROS, including superoxide, H 2 O 2 , and OH⅐, is associated with an active plant defense response and with aerobic respiration (44). ROS are highly toxic to all cellular components, and their rapid detoxification is essential for microbial survival.Xoo monofunctional catalase ...

show abstract

The generation of oxygen radicals during host plant responses to infection

Cited by 323 publications

References 111 publications

Reactive oxygen species and plant resistance to fungal pathogens

Reactive oxygen species and plant resistance to fungal pathogens

Generation of active oxygen in elicited cells of Arabidopsis thaliana is mediated by a NADPH oxidase‐like enzyme

Heterologous growth phase- and temperature-dependent expression and H2O2 toxicity protection of a superoxide-inducible monofunctional catalase gene from Xanthomonas oryzae pv. oryzae

Contact Info

Product

Resources

About