The genetic analysis of meiosis in female
            <i>Drosophila melanogaster</i>

Lindsley, Dan L.; Sandler, L.

doi:10.1098/rstb.1977.0019

Cited by 138 publications

(65 citation statements)

References 46 publications

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“…As mentioned above, Drosophila shows a marked suppression of crossing over near the tip of the X chromosome, spanning megabases of sequence (Lindsley and Sandler 1977). In contrast, our subtelomeric data come from a physical region of $100 kb.…”

Section: Resultsmentioning

confidence: 80%

“…Moreover, it has recently been reported that nonhomologous end joining (NHEJ) is a frequently used double-strand break repair pathway at the ends of human chromosomes (Linardopoulou et al 2005). Interestingly, while yeast exhibits a moderate reduction of homologous recombination in a small region of the telomere, D. melanogaster shows a more dramatic, large-scale suppression of crossing over within and proximal to telomeres (Lindsley and Sandler 1977). The tip of the X chromosome (distal 5%), in particular, shows markedly reduced crossing over.…”

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confidence: 99%

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Molecular Population Genetics of Drosophila Subtelomeric DNA

2008

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DNA sequence surveys in yeast and humans suggest that the forces shaping telomeric polymorphism and divergence are distinctly more dynamic than those in the euchromatic, gene-rich regions of the chromosomes. However, the generality of this pattern across outbreeding, multicellular eukaryotes has not been determined. To characterize the structure and evolution of Drosophila telomeres, we collected and analyzed molecular population genetics data from the X chromosome subtelomere in 58 lines of North American Drosophila melanogaster and 29 lines of African D. melanogaster. We found that Drosophila subtelomeres exhibit high levels of both structural and substitutional polymorphism relative to linked euchromatic regions. We also observed strikingly different patterns of variation in the North American and African samples. Moreover, our analyses of the polymorphism data identify a localized hotspot of recombination in the most-distal portion of the X subtelomere. While the levels of polymorphism decline sharply and in parallel with rates of crossing over per physical length over the distal first euchromatic megabase pairs of the X chromosome, our data suggest that they rise again sharply in the subtelomeric region (%80 kbp). These patterns of historical recombination and geographic differentiation indicate that, similar to yeast and humans, Drosophila subtelomeric DNA is evolving very differently from euchromatic DNA. P OPULATION geneticists aspire to understand the evolutionary forces shaping patterns of molecular polymorphism and divergence. The recent increase in the quantity of DNA sequence data from proteincoding regions has led to considerable advances in our understanding of the forces governing the evolution of such regions. For example, genomic surveys have revealed functional variants as well as classes of genes enriched for the signature of natural selection (e.g., Bustamante et al. 2005;Nielsen et al. 2005;Begun et al. 2007). In contrast, regions of the genome containing fewer genes but an increased density of repetitive sequences have been less amenable to population and molecular evolutionary analysis. These regions, typically found adjacent to telomeres and centromeres, are collectively referred to as the heterochromatin. Because heterochromatic DNA is difficult to clone, sequence, and assemble, much less is known about the structure of these regions. Thus, there is almost no foundation for effective population or comparative genomic analyses. Indeed, only recently have researchers begun to identify the sparsely distributed protein-coding sequences embedded in heterochromatin (reviewed in Yasuhara and Wakimoto 2006).Most population and molecular evolutionary surveys from telomeric regions come from yeast and primates (but see recent work on Arabidopsis in Kuo et al. 2006). These studies show that telomeric DNA is evolving very differently from the gene-rich euchromatin. For example, the telomeric regions of several human chromosomes are dramatically different from the corresponding regions in c...

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Section: Resultsmentioning

confidence: 80%

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confidence: 99%

Molecular Population Genetics of Drosophila Subtelomeric DNA

2008

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“…The cytological localization of jba and pm provides clues to estimate the location of the Lhr locus. Genetic distances should be proportional to the cytological distance especially in the middle portion of a chromosome arm (Lindsley and Sandler, 1977). The genetic map distance between Lhr and jba is 12.9, and Regions at which recombination occurred are indicated as , region between sd and jba; , region between jba and pm.…”

Section: Resultsmentioning

confidence: 99%

Cytogenetic mapping of the Lethal hybrid rescue gene of Drosophila simulans.

et al. 1997

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Hybrids not carrying Drosophila simulans X chromosome derived from the cross between D. melanogaster females and D. simulans males are lethal at the late third instar larval period or early pupal stage. This lethality can be rescued by the mutation Lethal hybrid rescue (Lhr) of D. simulans. The Lhr gene is considered to play an important role in reproductive isolation, but the genetic analyses have not been carried out extensively because of the lack of visible mutations and chromosome rearrangements in D. simulans. Using a cuticle mutation, jabara, of which locus is close to Lhr, and D. melanogaster deficiencies, we performed cytological mapping in hybrids and estimated the Lhr locus to 54E-F.

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“…Studies in fruit flies (Drosophila melanogaster) and other organisms have identified numerous genes with roles in homologous recombination (Lindsley and Sandler, 1977;Sekelsky et al, 2000). Among these are factors with a general role in homologous recombination including members of the Rad52 epistasis group such as the recombinase Rad51 (Ghabrial et al, 1998;Staeva-Vieira et al, 2003;Börner et al, 2004;McCaffrey et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Brca2/Pds5 complexes mobilize persistent meiotic recombination sites to the nuclear envelope

Kusch

2015

Journal of Cell Science

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Homologous recombination is required for reciprocal exchange between homologous chromosome arms during meiosis. Only select meiotic recombination events become chromosomal crossovers; the majority of recombination outcomes are noncrossovers. Growing evidence suggests that crossovers are repaired after noncrossovers. Here, I report that persisting recombination sites are mobilized to the nuclear envelope of Drosophila pro-oocytes during mid-pachytene. Their number correlates with the average crossover rate per meiosis. Proteomic and interaction studies reveal that the recombination mediator Brca2 associates with lamin and the cohesion factor Pds5 to secure persistent recombination sites at the nuclear envelope. In Rad51 2/2females, all persistent DNA breaks are directed to the nuclear envelope. By contrast, a reduction of Pds5 or Brca2 levels abolishes the movement and has a negative impact on crossover rates. The data suggest that persistent meiotic DNA double-strand breaks might correspond to crossovers, which are mobilized to the nuclear envelope for their repair. The identification of Brca2-Pds5 complexes as key mediators of this process provides a first mechanistic explanation for the contribution of lamins and cohesins to meiotic recombination.

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The genetic analysis of meiosis in female Drosophila melanogaster

Cited by 138 publications

References 46 publications

Molecular Population Genetics of Drosophila Subtelomeric DNA

Molecular Population Genetics of Drosophila Subtelomeric DNA

Cytogenetic mapping of the Lethal hybrid rescue gene of Drosophila simulans.

Brca2/Pds5 complexes mobilize persistent meiotic recombination sites to the nuclear envelope

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