The Genetic and Molecular Organization of the Dense Cluster of Functionally Related, Vital Genes in the DOPA Decarboxylase Region of the Drosophila melanogaster Genome

Wright, Theodore R. F.

doi:10.1007/978-3-540-47783-9_7

Cited by 20 publications

(14 citation statements)

References 72 publications

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“…In insects, melanization of the cuticle is common and contributes to the diverse coloration patterns that are the most visible features of the outer morphology. Most insights into the pathway that produces and incorporates melanin into the insect cuticle comes from studies in Drosophila melanogaster 2–4 . The melanin pathway starts with conversion of tyrosine to Dihydroxyphenylalanine (DOPA) by tyrosine hydroxylase (TH).…”

Section: Introductionmentioning

confidence: 99%

CRISPR-Cas9 targeted disruption of the yellow ortholog in the housefly identifies the brown body locus

Heinze

Kohlbrenner

Ippolito

et al. 2017

Sci Rep

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The classic brown body (bwb) mutation in the housefly Musca domestica impairs normal melanization of the adult cuticle. In Drosophila melanogaster, a reminiscent pigmentation defect results from mutations in the yellow gene encoding dopachrome conversion enzyme (DCE). Here, we demonstrate that the bwb locus structurally and functionally represents the yellow ortholog of Musca domestica, MdY. In bwb Musca strains, we identified two mutant MdY alleles that contain lesions predicted to result in premature truncation of the MdY open reading frame. We targeted wildtype MdY by CRISPR-Cas9 RNPs and generated new mutant alleles that fail to complement existing MdY alleles, genetically confirming that MdY is the bwb locus. We further found evidence for Cas9-mediated interchromosomal recombination between wildtype and mutant bwb alleles. Our work resolves the molecular identity of the classic bwb mutation in Musca domestica and establishes the feasibility of Cas9-mediated genome editing in the Musca model.

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Section: Introductionmentioning

confidence: 99%

CRISPR-Cas9 targeted disruption of the yellow ortholog in the housefly identifies the brown body locus

Heinze

Kohlbrenner

Ippolito

et al. 2017

Sci Rep

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“…Mutations in class 11, the noncomplementing class that includes null alleles, and class I11 (which also corresponds to basic isoform variants) cause phenotypes that appear to be associated with loss of a pterin cofactor [Brunet, 1980;Wright, 1987;Reynolds and ODonnell, 19871. Homozygous embryos from these classes die during late embryogenesis with completely unpigmented and weak cuticles (Fig.…”

Section: Embryonic Morphology Of Pu Mutantsmentioning

confidence: 99%

Molecular and developmental genetics of the Punch locus, a pterin biosynthesis gene in Drosophila melanogaster

1989

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Punch (Pu), the gene encoding the pterin biosynthetic enzyme GTP cyclohydrolase in Drosophila, is a complex locus. Mutations fall into several complementation classes that correspond to classes of mutants with distinct morphological and protein phenotypes. Two of these classes are developmentally specific, with mutants in each having defects in discrete subsets of the known functions of the locus. Defined functions of the locus include a role in embryonic nuclear divisions using initially a maternal Pu product, the synthesis of pterin cofactors that are required for catecholamine biosynthesis beginning in late embryogenesis, and the production of pterin-screening pigments in the developing adult eye. Mutant phenotypes include an interruption in synchronous nuclear divisions in precellular blastoderm embryos, a segment pattern phenotype in late embryos, failure to pigment and cross-link embryonic cuticular structures and failure to synthesize red eye pigments. Molecular analysis reveals that the locus is large, a minimum of 29 kb as defined by Southern mapping of Pu mutants. This region is transcriptionally extremely active, encoding at least 16 developmentally regulated transcripts. One transcript has been shown to be responsible for the production of the adult eye GTP cyclohydrolase on the basis of developmental profile, location with respect to the mapping of eye-specific Pu mutants, absence in eye-specific mutants, and hybrid-selection in vitro translation experiments. Several other transcripts are candidates for Pu vital functions, as suggested by their pattern of expression and their derivation from regions to which lethal Pu mutations map.

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“…More recently a combination of classical cytogenetic and molecular biological techniques has been used to analyze de®ned chromosomal regions and the ®ne structure of individual bands and interbands. As a result of these studies it is now understood that individual bands of polytene chromosomes may contain one gene (Rykowsky et al 1988), two genes (Ish-Horowicz and Pinchin 1980), several functionally related genes (Ayme and Tissieres 1985;Wright 1987;Stathakis et al 1995) or even dozens of repeated genes (Lifton et al 1978;De Lotto et al 1982). Some of the chromomeres of polytene chromosomes may contain large amounts of regulatory sequences in addition to coding DNA (Peifer et al 1987).…”

Section: Introductionmentioning

confidence: 99%

Molecular organization of an individual Drosophila polytene chromomere: transcribed sequences in the 10A1-2 band

1997

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Complete cloning of the DNA in the cytogenetic region 9F12-10A7 has allowed us to analyze the fine structure of an individual polytene chromomere in Drosophila melanogaster. In this report we identify the transcriptionally active fragments within the 10A1-2 band. At least 22 DNA fragments representing multiple distinct transcription units are active during embryogenesis, in third-instar larva or in isolated larval tissues. The transcribed fragments are significantly (at least sevenfold) more numerous than the three genetically defined complementation groups in the 10A1-2 band. One of the transcriptionally active zones detected encompasses a previously unsuspected homologue of the genes Delta and Serrate.

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The Genetic and Molecular Organization of the Dense Cluster of Functionally Related, Vital Genes in the DOPA Decarboxylase Region of the Drosophila melanogaster Genome

Cited by 20 publications

References 72 publications

CRISPR-Cas9 targeted disruption of the yellow ortholog in the housefly identifies the brown body locus

CRISPR-Cas9 targeted disruption of the yellow ortholog in the housefly identifies the brown body locus

Molecular and developmental genetics of the Punch locus, a pterin biosynthesis gene in Drosophila melanogaster

Molecular organization of an individual Drosophila polytene chromomere: transcribed sequences in the 10A1-2 band

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