The genetic architecture of phosphorus efficiency in sorghum involves pleiotropic QTL for root morphology and grain yield under low phosphorus availability in the soil

Bernardino, Karine Costa; Pastina, Maria Marta; Menezes, C. B. de; Sousa, S. M. de; Maciel, Laiane S.; Carvalho, G. M. C.; Guimarães, C. T.; Barros, B. A.; Silva, Luciano da Costa e; Carneiro, Pedro Crescêncio Souza; Schaffert, R. E.; Kochian, Leon V.; Magalhães, J. V. de

doi:10.1186/s12870-019-1689-y

Cited by 62 publications

(83 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phosphorus (P) is one of the most vital nutrient elements in crop output and quality formation, not only presenting in major organic molecules (e.g., RNA, DNA, ATP, and membrane phospholipids) but also playing important roles in photosynthesis, energy metabolism, enzymatic reactions, and sugar metabolism (Péret et al 2011;Qiu et al 2013). P is the second most limiting nutrient for crop production after N (Bernardino et al 2019). A large amount of P fertilizer has been used to achieve higher crop yields and ensure food security worldwide (Dhillon et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Detection of QTL for phosphorus efficiency and biomass traits at the seedling stage in wheat

Yang

Zhang

Zheng

et al. 2020

CEREAL RESEARCH COMMUNICATIONS

View full text Add to dashboard Cite

Phosphorus (P) is one of the most vital nutrient elements in crop output and quality formation. In this study, four biomass, four P uptake efficiency (PupE), and three P-utilization efficiency (PutE) traits were investigated using a set of recombinant inbred lines (RILs) derived from a cross of "SN0431 × LM21", under hydroponic culture trials at low P (LP) and normal P (NP) levels in two different seasons, respectively. A total of 85 QTL were identified on 18 chromosomes except for 1D, 2A, and 3D. Among them, 36 and 42 QTL were detected under LP and NP, respectively, and seven QTL were simultaneously detected under LP and NP. Seventeen relatively high-frequency QTL (RHF-QTL) were detected. The average contributions of 13 major RHF-QTL were over 10.00%. Five important QTL clusters were detected on chromosomes 4D, 5A, and 5B. Among them, positive linkages were observed between PutE and biomass traits at four QTL clusters, C1, C2, C3, and C6, showing these loci may be hot spots for genetic control of both phosphorus utilization and biomass accumulation in wheat seedlings. In addition, correlation analysis indicated that three biomass traits (SDW, RDW, and TDW) should be used as primary selection indexes for PE at the seedling stage.

show abstract

Section: Introductionmentioning

confidence: 99%

Detection of QTL for phosphorus efficiency and biomass traits at the seedling stage in wheat

Yang

Zhang

Zheng

et al. 2020

CEREAL RESEARCH COMMUNICATIONS

View full text Add to dashboard Cite

show abstract

“…In this study, these sorghum SbPSTOL1 genes appear to modify root system morphology and architecture, leading to increases in grain yield in field studies on a low-P Brazilian soil, and also exhibited enhanced biomass accumulation and P content in sorghum landraces from West Africa using native soils. These data suggest a stable effect of the target alleles across environments and sorghum genetic backgrounds (Hufnagel et al, 2014;Bernardino et al, 2019). In maize, homologs of OsPSTOL1 that were preferentially expressed in roots and co-localized with QTLs associated with root morphology and P acquisition traits (Azevedo et al, 2015), mapped in the same region as QTLs for grain yield on a low-P soil (Mendes et al, 2014).…”

Section: Phosphorus-starvation Tolerance 1 (Pstol1) Genesmentioning

confidence: 78%

Root Adaptation via Common Genetic Factors Conditioning Tolerance to Multiple Stresses for Crops Cultivated on Acidic Tropical Soils

et al. 2020

Self Cite

View full text Add to dashboard Cite

Crop tolerance to multiple abiotic stresses has long been pursued as a Holy Grail in plant breeding efforts that target crop adaptation to tropical soils. On tropical, acidic soils, aluminum (Al) toxicity, low phosphorus (P) availability and drought stress are the major limitations to yield stability. Molecular breeding based on a small suite of pleiotropic genes, particularly those with moderate to major phenotypic effects, could help circumvent the need for complex breeding designs and large population sizes aimed at selecting transgressive progeny accumulating favorable alleles controlling polygenic traits. The underlying question is twofold: do common tolerance mechanisms to Al toxicity, P deficiency and drought exist? And if they do, will they be useful in a plant breeding program that targets stress-prone environments. The selective environments in tropical regions are such that multiple, co-existing regulatory networks may drive the fixation of either distinctly different or a smaller number of pleiotropic abiotic stress tolerance genes. Recent studies suggest that genes contributing to crop adaptation to acidic soils, such as the major Arabidopsis Al tolerance protein, AtALMT1, which encodes an aluminum-activated root malate transporter, may influence both Al tolerance and P acquisition via changes in root system morphology and architecture. However, trans -acting elements such as transcription factors (TFs) may be the best option for pleiotropic control of multiple abiotic stress genes, due to their small and often multiple binding sequences in the genome. One such example is the C2H2-type zinc finger, AtSTOP1, which is a transcriptional regulator of a number of Arabidopsis Al tolerance genes, including AtMATE and AtALMT1 , and has been shown to activate AtALMT1 , not only in response to Al but also low soil P. The large WRKY family of transcription factors are also known to affect a broad spectrum of phenotypes, some of which are related to acidic soil abiotic stress responses. Hence, we focus here on signaling proteins such as TFs and protein kinases to identify, from the literature, evidence for unifying regulatory networks controlling Al tolerance, P efficiency and, also possibly drought tolerance. Particular emphasis will be given to modification of root system morphology and architecture, which could be an important physiological “hub” leading to crop adaptation to multiple soil-based abiotic stress factors.

show abstract

“…Plant grows adapting to P de ciency environment covers a series of gene expressions and morphophysiological events [4], such as regulation of P transporters (PTs), mycorrhizal association, phosphatase secretion, organic acid exudation and alteration in root structure under low-P conditions [5]. Studies have shown that OsPHR2, homologous to AtPHR1 in Arabidopsis, is a major transcriptional regulator of low P response in rice [6,7] which could active the Pi starvation-induced genes through binding to the P1BS (PHR1 Binding Sequence; GNATATNC) motif presented in genes' promoter region, including PHT1 members [8,9].…”

Section: Introductionmentioning

confidence: 99%

Identification of phosphorus stress related proteins in the seedlings of Dongxiang wild rice (Oryza rufipogon Griff.) using label-free quantitative proteomic analysis

Deng¹,

Bai²,

Dai³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The lack of available phosphorus (P) in soil is one of the important factors restricting rice growth. Previous studies indicated that Dongxiang wild rice (DXWR, O. rufipogon Griff.) was resistant to low-P stress. So far, the research of resistance mechanism in DXWR was very limited.Results: The results showed that 3589 significant differential accumulation proteins were identified between low P and the normal P treated root samples. 60 up-regulated and 15 down-regulated proteins were identified with ≥ 1.5-fold changes as an additional standard. Furthermore, among 75 significantly different expression proteins (SDEPs), 24 proteins also detected in previous transcriptome dataset verified by qRT-PCR, including OsPT2, OsPT8, OsPAP10c, OsPAP10a and OsPHF1. Through comprehensive analysis, it was found that DXWR could increase PAPs, membrane location of PTs, rhizosphere area, alternative splicing and decrease ROS activity to deal with low-P stress. Moreover, among the genes corresponding to 75 SDEPs, 7 uncharacterized genes were located in previous P related QTL intervals, of which two genes (LOC_Os12g09620 and LOC_Os03g40670) have been detected at both transcriptome and proteome levels. In addition, the expression patterns of OsPHR1, OsPHR2, OsPHO1 and NAT-OsPHO1 in DXWR were different in cultivated rice, suggesting that the response mechanism of some low-P tolerance in DXWR might be different from that in cultivated rice.Conclusions: This study screened out some candidate genes for low-P resistance and preliminarily verified that there might be a different low-P response mechanism in DXWR than in cultivated rice, which would provide insights in cloning the P-deficiency genes from wild rice, as well as elucidating the molecular mechanism of low-P resistance in DXWR.

show abstract

The genetic architecture of phosphorus efficiency in sorghum involves pleiotropic QTL for root morphology and grain yield under low phosphorus availability in the soil

Cited by 62 publications

References 65 publications

Detection of QTL for phosphorus efficiency and biomass traits at the seedling stage in wheat

Detection of QTL for phosphorus efficiency and biomass traits at the seedling stage in wheat

Root Adaptation via Common Genetic Factors Conditioning Tolerance to Multiple Stresses for Crops Cultivated on Acidic Tropical Soils

Identification of phosphorus stress related proteins in the seedlings of Dongxiang wild rice (Oryza rufipogon Griff.) using label-free quantitative proteomic analysis

Contact Info

Product

Resources

About