The genetic control of alkaline phosphatase activity in the duodenum of the mouse

The Differentiation of Alkaline Phosphatase in the Small Intestine

“…The quantity of such phosphatase, and its ratio, differs between mouse strains, being relatively abundant and having ratios as high as 3, in our high-activity strain (Nayudu & Moog, 1967). This activity resembles HRP in that it does not bind the DEAEcellulose equilibrated with 0.05 M tris acetate at pH 7.4; thus, it is completely separable from the major fraction (used in the titration experiments described in the preceding paragraph), which appears as a peak with ratios below 1 through- out.…”

“…That the phosphatase of the mouse duodenum is heterogeneous at all stages has been decisively demonstrated by investigations employing column chromatography and starch gel electrophoresis (Moog, Vire & Grey, 1966;Nayudu & Moog, 1967;Etzler & Moog, 1968b). All this work has been based on homogenates of whole duodenal (or sometimes jejunal) wall, in which the phosphatase has been solubilized with butanol (Morton, 1954).…”

Clinical Chemistry of the Laboratory Mouse

“…lAP is located along the brush-border of the enterocytes. Intestinal AP activity was shown to vary four-fold between two strains of Swiss mice (Nayudu and Moog 1967). This difference in activity was under polygenic control and influenced by a strain-specific factor in milk.…”

Energy transduction at the catalytic site of enzymes: Hydrolysis of phosphoester bonds and synthesis of pyrophosphate by alkaline phosphatase

“…Alkaline phosphatase (EC 3.1.3.1) was purified from mouse intestinal epithelial cells by butanol solubilization, alcohol fractionation, and molecular-sieve chromatography, followed by affinity chromatography with L-histidyl diazobenzylphosphonic acid agarose gel [13,14].…”

“…The hydrolysis of pyrophosphate, ADP, glycerol phosphate and glucose 6-phosphate was assayed by measuring the release of Pi colorimetrically [17]. The hydrolysis of p-nitrophenylphosphate was assayed by measuring the formation of p-nitrophenyl colorimetrically at 405 nm [14].…”