The genetic control of grain protein content variation in a doubled haploid population derived from a cross between Australian and North American two-rowed barley lines

Emebiri, Livinus; Moody, D. Branch; Horsley, Richard D.; Panozzo, JF; Read, B. J.

doi:10.1016/j.jcs.2004.08.012

Cited by 35 publications

(31 citation statements)

References 28 publications

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“…The identified markers for GPC will be very useful in the evaluation and screening of barley accessions with reasonable GPC. In comparison with previous studies [1,4,15,31], we found more markers in this study, including 3, 3, and 1 marker(s) on chromosome 6H, 2H and 5H, respectively (Table 2). Three major QTLs were identified on chromosomes 6H and 2H using a barley mapping population developed from a cross between ‘Karl’, a low grain protein six-rowed variety and ‘Lewis’, a high grain protein two-rowed variety.…”

Section: Discussioncontrasting

confidence: 74%

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Grain protein content variation and its association analysis in barley

Cai

Chen

et al. 2013

BMC Plant Biol

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BackgroundGrain protein content (GPC) is an important quality determinant for barley used as malt, feed as well as food. It is controlled by a complex genetic system. GPC differs greatly among barley genotypes and is also variable across different environments. It is imperative to understand the genetic control of barley GPC and identify the genotypes with less variation under the different environments.ResultsIn this study, 59 cultivated and 99 Tibetan wild barley genotypes were used for a genome-wide association study (GWAS) and a multi-platform candidate gene-based association analysis, in order to identify the molecular markers associated with GPC. Tibetan wild barley had higher GPC than cultivated barley. The significant correlation between GPC and diastatic power (DP), and malt extract confirmed the importance of GPC in determining malt quality. Diversity arrays technology (DArT) markers associated with barley GPC were detected by GWAS. In addition, GWAS revealed two HvNAM genes as the candidate genes controlling GPC. No association was detected between HvNAM1 polymorphism and GPC, while a single nucleotide polymorphism (SNP) (798, P < 0.01), located within the second intron of HvNAM2, was associated with GPC. There was a significant correlation between haplotypes of HvNAM1, HvNAM2 and GPC in barley.ConclusionsThe GWAS and candidate gene based-association study may be effectively used to determine the genetic variation of GPC in barley. The DArT markers and the polymorphism of HvNAM genes identified in this study are useful in developing high quality barley cultivars in the future. HvNAM genes could play a role in controlling barley GPC.

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Section: Discussioncontrasting

confidence: 74%

“…Barley GPC is under polygenic control, with many quantitative trait loci (QTLs) having been mapped on all seven chromosomes, mainly on 2H, 4H, 5H and 6H [3,4]. All these loci had been determined by QTL mapping.…”

Section: Introductionmentioning

confidence: 99%

Grain protein content variation and its association analysis in barley

Cai

Chen

et al. 2013

BMC Plant Biol

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“…On the other hand, insufficient levels of grain protein may limit the growth of yeast during the fermentation process; it also reduces the stability of the beer head because the beer foam cannot cling effectively to the side of the glass. Consequently, maltsters prefer a GPC close to 10.5% [90]. It behooves the breeder to know which parameters are correlated, because these parameters require balancing to achieve the optimal outcome.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Mapping for Kernel and Malting Quality Traits Using Historical European Barley Records

et al. 2014

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Malting quality is an important trait in breeding barley (Hordeum vulgare L.). It requires elaborate, expensive phenotyping, which involves micro-malting experiments. Although there is abundant historical information available for different cultivars in different years and trials, that historical information is not often used in genetic analyses. This study aimed to exploit historical records to assist in identifying genomic regions that affect malting and kernel quality traits in barley. This genome-wide association study utilized information on grain yield and 18 quality traits accumulated over 25 years on 174 European spring and winter barley cultivars combined with diversity array technology markers. Marker-trait associations were tested with a mixed linear model. This model took into account the genetic relatedness between cultivars based on principal components scores obtained from marker information. We detected 140 marker-trait associations. Some of these associations confirmed previously known quantitative trait loci for malting quality (on chromosomes 1H, 2H, and 5H). Other associations were reported for the first time in this study. The genetic correlations between traits are discussed in relation to the chromosomal regions associated with the different traits. This approach is expected to be particularly useful when designing strategies for multiple trait improvements.

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“…Protein content is the most important quality trait in barley. The protein content of barley affects the malting and brewing processes, and the operating efficiency of the brewery and the quality of malt used for making beer [30], [31]. In barley, two protein coding genes, HvNAM-1 and HvNAM-2 , have been identified and mapped on chromosomes 6H and 2H, respectively [32], [33].…”

Section: Introductionmentioning

confidence: 99%

Tibet as a Potential Domestication Center of Cultivated Barley of China

et al. 2013

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The importance of wild barley from Qinghai-Tibet Plateau in the origin and domestication of cultivated barley has long been underestimated. Population-based phylogenetic analyses were performed to study the origin and genetic diversity of Chinese domesticated barley, and address the possibility that the Tibetan region in China was an independent center of barley domestication. Wild barley (Hordeum vulgare ssp. spontaneum) populations from Southwest Asia, Central Asia, and Tibet along with domesticated barley from China were analyzed using two nuclear genes. Our results showed that Tibetan wild barley distinctly diverged from Southwest Asian (Near East) wild barley, that Central Asian wild barley is related to Southwest Asian wild barley, and that Chinese domesticated barley shares the same haplotypes with Tibetan wild barley. Phylogenetic analysis showed a close relationship between Chinese domesticated barley and the Tibetan wild barley, suggesting that Tibetan wild barley was the ancestor of Chinese domesticated barley. Our results favor the polyphyletic origin for cultivated barley.

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The genetic control of grain protein content variation in a doubled haploid population derived from a cross between Australian and North American two-rowed barley lines

Cited by 35 publications

References 28 publications

Grain protein content variation and its association analysis in barley

Grain protein content variation and its association analysis in barley

Genome-Wide Association Mapping for Kernel and Malting Quality Traits Using Historical European Barley Records

Tibet as a Potential Domestication Center of Cultivated Barley of China

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