The genome of bacteriophage T4.

Wood, William B.; Revel, Helen R.

doi:10.1128/mmbr.40.4.847-868.1976

Cited by 123 publications

(67 citation statements)

References 47 publications

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“…Identification of genes 55, o!gt, 47 and 46 On the basis of mapping data (Wood and Revel, 1976;Niggemann et al, 1981;Spicer and Konigsberg, 1983) genes aogt, 47, 46, and possibly gene 55, were expected to be found within the DNA region studied here. To identify these genes, selected subclones used during the sequencing procedure were subjected to marker rescue tests.…”

Section: Resultsmentioning

confidence: 82%

“…In vivo and in vitro studies have indicated that T4 genes 55 through to 46 are likely to be co-transcribed, starting from a promoter ahead or in close vicinity of gene 55 (Hercules and Sauerbier, 1973;Gram et al, 1984). This DNA region, -7 kb in length, harbours the genes 55, 47 and 46 (which are essential for the T4 development) and the non-essential genes gor2 and oagt (Wood and Revel, 1976).…”

Section: Introductionmentioning

confidence: 99%

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Genes 55, alpha gt, 47 and 46 of bacteriophage T4: the genomic organization as deduced by sequence analysis.

Gram¹,

Rüger²

1985

The EMBO Journal

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The nucleotide sequence of T4 genes 55, alpha gt, 47 and 46 was determined by a combination of ‘classical’ procedures and a shotgun approach. Small DNA fragments generated by frequent cleavage with restriction enzymes or by sonication of restriction fragments were cloned in phage M13 vectors and sequenced by the dideoxy method. The positions of the genes were determined by marker rescue between the corresponding T4 amber mutants and the cloned T4 DNA fragments used in the sequencing experiments. The sequence gives an insight into the organization of this 7.1‐kb early region of the T4 genome and shows that genetically ‘silent’ portions within this region are not void of genetic information.

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Section: Resultsmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Genes 55, alpha gt, 47 and 46 of bacteriophage T4: the genomic organization as deduced by sequence analysis.

Gram¹,

Rüger²

1985

The EMBO Journal

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“…The protein composition of the tail fibers of phages T2, Ox2 and K3 is shown in fig.1. Gene product (gp) 38 of T4 phage apparently modifies a tail fiber protein without assembling to the tail fiber [2]. In contrast, gp38 of T2, Ox2 and K3 is a constituent of the respective tail fibers.…”

Section: Introductionmentioning

confidence: 99%

Predicted structure of tail‐fiber proteins of T‐even type phages

1987

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The sequences of the tail fiber protein 36 of the phages T 4, T 2, K3, and Ox 2 were analyzed for homologies and for folding patterns using structure prediction methods. No repeating motif was found. A model for the fiber structure is proposed in which fl-strands of about 6 amino acids are separated by turns. In the fl-strand, hydrophobic amino acids are found alternating with hydrophilic ones. Such amphipathic fl-strands can be stabilized by dimer formation. The dimerization occurs in a parallel fashion so that both N-termini are at one end of the dimer. This structure represents a rigid fiber. Our model is consistent with electron microscopic data and electron diffraction patterns for the T 4 tail fiber. The observation that all fiber components are found as dimers supports our model. Sequences of the receptor recognition proteins 38 of T-even type phages reveal an architecture different from the architecture of the fiber proteins 36 and 37 of these phages.

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“…The position of this, in relation to various restriction enzyme sites, is shown in Figure 1. The position of the open reading frame corresponds well to the location of g63 determined by genetic mapping (Wood and Revel, 1976;O'Farrell et al, 1980). Eighteen clones cover the region of the open reading frame and sequence information was obtained from both strands of DNA except for 137 bp at its 3' end, a region from which it is difficult to obtain clones.…”

Section: Resultsmentioning

confidence: 99%

Sequence and cloning of bacteriophage T4 gene 63 encoding RNA ligase and tail fibre attachment activities.

Rand¹,

Gait²

1984

The EMBO Journal

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The sequence of gene 63 of bacteriophage T4 was determined by a shotgun approach. Small DNA fragments, derived by sonication of a restriction fragment that encompasses the region of gene 63, were cloned in M13 vectors and sequenced by the ‘dideoxy’ method. The position of the gene was established by comparison with the sequence of a gene 63 amber mutant. Knowledge of the DNA sequence of gene 63 and surrounding regions has allowed the construction of a clone of gene 63 in which RNA ligase production is under the control of the lac promoter of bacteriophage M13mp8. Infected E. coli cells can be induced to produce a protein indistinguishable from commercially available RNA ligase.

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The genome of bacteriophage T4.

Cited by 123 publications

References 47 publications

Genes 55, alpha gt, 47 and 46 of bacteriophage T4: the genomic organization as deduced by sequence analysis.

Genes 55, alpha gt, 47 and 46 of bacteriophage T4: the genomic organization as deduced by sequence analysis.

Predicted structure of tail‐fiber proteins of T‐even type phages

Sequence and cloning of bacteriophage T4 gene 63 encoding RNA ligase and tail fibre attachment activities.

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