The Genome of Simian Virus 40

Abstract

The nucleotide sequence of SV40 DNA was determined, and the sequence was correlated with known genes of the virus and with the structure of viral messenger RNA's. There is a limited overlap of the coding regions for structural proteins and a complex pattern of leader sequences at the 5' end of late messenger RNA. The sequence of the early region is consistent with recent proposals that the large early polypeptide of SV40 is encoded in noncontinguous segments of DNA.

Heritable lympho-epithelial thymoma resulting from a transgene insertional mutation

“…The SV40 early region transcription terminator/polyadenylation site (nucleotide residues #2586 ± 2452, see Reddy et al, 1978) was inserted downstream from the TGF insert. The MHC ± TGF insert was puri®ed from an agarose gel using Geneclean glass beads (Bio 101, Vista, CA, USA) and microinjected into inbred C3HeB/FeJ (Jackson Laboratories, Bar Harbor, MA, USA) zygotes using standard methodologies (Hogan, 1994).…”

Biosynthesis of peptide precursors and protease inhibitors using new constitutive and inducible eukaryotic expression vectors

“…Prior to ligation the SaiI site had been converted to a BamHI site by BamHI linkers. The simian virus 40 (SV40) polyadenylation signal, a 237 bp BamHI to BciI fragment corresponding to position 2451-2688 of the SV40 genome [12], was inserted into the BamHI site of this vector in two different orientations, thereby regenerating one BamHI site. This fragment contains two polyadenylation signals on one strand and one on the other [12].…”

“…The simian virus 40 (SV40) polyadenylation signal, a 237 bp BamHI to BciI fragment corresponding to position 2451-2688 of the SV40 genome [12], was inserted into the BamHI site of this vector in two different orientations, thereby regenerating one BamHI site. This fragment contains two polyadenylation signals on one strand and one on the other [12]. In the vector where the regenerated BamHI site was located between the polylinker and the polyadenylation signal, the polylinker was extended with EcoRI and SmaI by insertion of a 24 bp BamHI/BgAI fragment from the polylinker of pSP6/T3 (Pharmacia) into the BamHI site.…”

“…enhancer, and origin of DNA replication were excised as a 326 bp Hind111 to PvuII fragment corresponding to position 5085 to 189 of the SV40 genome [12]. This fragment was converted to blunt ends and ligated into the blunt end converted Bg/II site of pTEJ-4.…”

“…The neomycin phosphotransferase encoding gene is driven by the SV40 early promoter. In addition to the promoter, the 326 bp HindIII-PvuII SV40 fragment also contains the origin of DNA replication [ 12,22,29]. This should allow this vector to replicate to a level greater than lo5 copies per cell when transfected into e.g.…”