The genome sequence of a caddisfly, Limnephilus lunatus (Curtis, 1834)

Abstract

We present a genome assembly from an individual Limnephilus lunatus (a caddisfly; Arthropoda; Insecta; Trichoptera; Limnephilidae). The genome sequence is 1,270 megabases in span. Most of the assembly is scaffolded into 13 chromosomal pseudomolecules, including the assembled Z chromosome. The mitochondrial genome has also been assembled and is 15.4 kilobases long.

Characterization of the primary structure of the major silk gene, h-fibroin, across caddisfly (Trichoptera) suborders

“… 980/n.a. C:91.4%[S:89.0%,D:2.4%],F:6.0%,M:2.6% Limnephilus lunatus (DtoL) 42 Integripalpia (tube case) GCA_917563855.2 1,269,651,477 18,993,099/95,392,806 139/39 C:89.7%[S:88.9%,D:0.8%],F:7.4%,M:2.9% Limnephilus marmoratus (DtoL) 43 Integripalpia (tube case) GCA_917880885.1 1,629,971,709 8,018,677/56,174,236 395/68 C:90.4%[S:89.3%,D:1.1%],F:6.7%,M:2.9% Limnephilus rhombicus (DtoL) Integripalpia (tube case) GCA_929108145.2 1,578,808,083 10,796,652/54,234,467 272/62 C:89.8%[S:88.6%,D:1.2%],F:7.1%,M:3.1% Leptonema lineaticorne a Annulipalpia (retreat) GCA_024500535.1 273,010,349 13,827,090/n.a. 65/n.a.…”

“…In this study, we report eight new full-length h-fibroin sequences for caddisflies across a diverse set of silk usage. Two of these were generated from new genomic resources, whereas five were mined from previously published genomes, 41 , 42 , 43 , 44 highlighting the relevance of genome sequencing projects for the wider scientific community. The new full-length h-fibroins represent a substantial increase in the number of genomic resources available for the study of caddisfly silk and allowed us to compare the major silk gene for eleven species across the primary clades of Trichoptera, in which species exhibit different silk usage.…”

“… 48 GenBank Accession: GCA_936435175.1 Glyphotaelius pellucidus h-fibroin This study GenBank Accession: BK063450 Hesperophylax magnus genome assembly Hotaling et al. 42 GenBank Accession: GCA_026573805.1 Hesperophylax magnus h-fibroin Hotaling et al. 42 GenBank Accession: OQ787679 Limnephilus lunatus genome assembly Austin et al.…”

“… 42 GenBank Accession: GCA_026573805.1 Hesperophylax magnus h-fibroin Hotaling et al. 42 GenBank Accession: OQ787679 Limnephilus lunatus genome assembly Austin et al. 46 GenBank Accession: GCA_917563855.2 Limnephilus lunatus h-fibroin This study GenBank Accession: BK063451 Limnephilus marmoratus genome assembly Clifford et al.…”

Comprehensive analysis of silk proteins and gland compartments in Limnephilus lunatus, a case-making trichopteran

“…We manually curated the transcript sequences of the candidate silk genes in L. lunatus against the available genomic sequences (GenBank:GCA_917563855.2) [ 27 ] using BLAST searches [ 28 ]. For easier distinguishing of exon–intron boundaries and visualization of genome clusters, we mapped the Illumina reads to the genome using RNA STAR software (Galaxy version 2.7.8a + galaxy0) in combination with IGV 2.9.4 [ 20 ].…”