We present a genome assembly from an individual female Golden-tabbed robberfly, Eutolmus rufibarbis (Arthropoda; Insecta; Diptera; Asilidae). The genome sequence has a total length of 285.90 megabases. Most of the assembly is scaffolded into 7 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 16.22 kilobases in length.