The genome sequence of the Buff-tailed Bumblebee, Bombus terrestris (Linnaeus, 1758)

Abstract

We present a genome assembly from an individual female Bombus terrestris (the Buff-tailed Bumblebee; Arthropoda; Insecta; Hymenoptera; Apidae). The genome sequence is 393.0 megabases in span. Most of the assembly is scaffolded into 18 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 24.7 kilobases in length. Gene annotation of this assembly on Ensembl identified 14,435 protein coding genes.

Klumpy: A Tool to Evaluate the Integrity of Long-Read Genome Assemblies and Illusive Sequence Motifs

“…As the BLASTN results are largely composed of sequences from the genus Bombus , we speculate that LOC126875579 originated from an ancestral Bombus species. It must be noted that several of the Bombus data sets used in this study have been documented to share a common 10-mer amongst other Bombus genome assemblies (Crowley et al 2023b), with our results exemplifying a similar case. Despite these details, these chromosome-level genome assemblies remain useful and with the sequencing of additional bee species, the extent of which LOC126875579 can be found across Bombus and relatives can be revealed.…”

“…To identify orthologs, we used BLASTN to search the NCBI NR database using the LOC126875579 exons as the query. In light of the BLASTN results, we searched the genome assemblies of B. affinis (GCF_024516045.1; Koch et al 2023), B. hortorum (GCA_905332935.1; Crowley 2021), B. hypnorum (GCA_911387925.2; Crowley et al 2023a), B. pratorum (GCA_930367275.1; Crowley et al 2023c), B. sylvestris (GCA_911622165.2; Crowley 2023), and B. terrestris (GCF_910591885.1; Crowley et al 2023b) for LOC126875579 klumps. To keep our analysis consistent across all bee species, we generated klumps using the same parameters applied to B. huntti .…”