The genomic RNA of Mengovirus II: Comparative analysis of the standard and denser virions

Perez-Bercoff, R.; Gander, M; Preisig, Elisabeth

doi:10.1016/0042-6822(78)90446-4

Cited by 13 publications

(8 citation statements)

References 20 publications

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“…HHA viral RNA was digested to completion with RNase T1, the resulting fragments were labeled at the 5' end with polynucleotide kinase and [y-32P]ATP, and the large T1resistant oligonucleotides were resolved by two-dimensional polyacrylamide gel electrophoresis as previously described (22). The complexity of the fingerprint depicted in Fig.…”

Section: Resultsmentioning

confidence: 99%

Isolation and molecular cloning of a fast-growing strain of human hepatitis A virus from its double-stranded replicative form

Venuti¹,

Russo²,

Grosso³

et al. 1985

J Virol

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A fast-growing strain of human hepatitis A virus was selected and characterized. The virus has the unusual property of developing a strong cytopathic effect in tissue culture in 7 to 10 days. Sequences of the viral genome were cloned into recombinant plasmids with the double-stranded replicative form as a template for the reverse transcription of cDNA. Restriction analysis and direct sequencing indicate that this strain is different from that described by Ticehurst et al. (Proc. Natl. Acad. Sci. USA 80:5885-5889, 1983) in the region that presumptively codes for the major capsid protein VP1, but both isolates have conserved large areas of homology in the untranslated 5'-terminal sequences of the genome.

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Section: Resultsmentioning

confidence: 99%

Isolation and molecular cloning of a fast-growing strain of human hepatitis A virus from its double-stranded replicative form

Venuti¹,

Russo²,

Grosso³

et al. 1985

J Virol

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“…particles of higher buoyant density (density 1-44 g/ml) in CsC1 than standard particles (density 1.33 g/ml), have been described for several picornaviruses: poliovirus, coxsackie B5 virus, mengovirus, bovine enterovirus, and swine vesicular disease virus (SVDV) (Rowlands et at., 1975;Yamaguchi-Koll et al, 1975;Wiegers et al, 1977;Perez-Bercoff et al, 1978). Antigenic properties and chemical properties, such as amount of RNA, polypeptide composition, and RNA to protein ratio, are similar for dense and standard particles.…”

mentioning

confidence: 92%

“…Antigenic properties and chemical properties, such as amount of RNA, polypeptide composition, and RNA to protein ratio, are similar for dense and standard particles. It was suggested (Rowlands et al, 1975;Perez-Bercoff et al, 1978) that higher buoyant density results from conformational differences between dense and standard particles, dense particles having a more open capsid structure leading to a greater interaction of virus RNA with Cs + ions (Rowlands et al, 1975;Yamaguchi-Koll et al, 1975;Wiegers et aL, 1977Wiegers et aL, , 1978Mapoles et al, 1978).…”

mentioning

confidence: 99%

Echovirus 11 Dense Particles: Isolation and Preliminary Characterization

Cova-Baczko¹,

Aymard²

1982

Journal of General Virology

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SUMMARYDense particles (density 1.44 g/ml) were isolated during purification in CsC1 of echovirus 11 produced in HeLa cells. The dense particles had similar antigenic properties and similar RNA and protein composition to standard (density 1.33 g/ml) echovirus 11 particles. They differed from standard particles in their higher buoyant density, lower infectivity and slightly smaller diameter. In contrast to other picornavirus dense particles, echovirus 11 dense particles were present as a major component of virus population. This high ratio of dense particles was found only in virus from HeLa cells which produced non-haemagglutinating echovirus l l. Haemagglutinating echovirus i 1 from primary monkey kidney (MK) cells did not contain any detectable levels of dense particles.

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“…The RNAs of the aphthoand cardioviruses differ from those of the entero-and rhinoviruses in containing a long tract (100 to 250 residues) which is resistant to RNAse T1 (2,3). This tract comprises c. 90% C residues and hence has been termed poly C. It is located towards the 5' end of the RNA in EMC and Mengo viruses (4,5) and FMDV (6). However its precise location has been determined for only one virus strain, FMDV, type A, where it was found to be 400-500 bases from the 5' end (7).…”

Section: Introductionmentioning

confidence: 99%

Sequence and location of the poly C tract in aphtho- and cardiovirus RNA

et al. 1979

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The poly C tract in the RNA of the aphtho- and cardio viruses has been examined in several isolates of foot-and-mouth disease virus (FMDV) and encephalomyocarditis (EMC) virus. The length of the tract is variable, containing 100 to 170 bases in the FMDV isolates and 80 to 250 bases in the EMC virus isolates. Each poly C tract contains c. 10% A and U residues, located at the 5' end, i.e. most of the tract is a continuous run of C residues. The position of the tract on the genome was the same in each of the FMDV isolates, about 400 bases from the 5' end, whereas in the EMC virus isolates it was about 150 bases from the 5' end.

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The genomic RNA of Mengovirus II: Comparative analysis of the standard and denser virions

Cited by 13 publications

References 20 publications

Isolation and molecular cloning of a fast-growing strain of human hepatitis A virus from its double-stranded replicative form

Isolation and molecular cloning of a fast-growing strain of human hepatitis A virus from its double-stranded replicative form

Echovirus 11 Dense Particles: Isolation and Preliminary Characterization

Sequence and location of the poly C tract in aphtho- and cardiovirus RNA

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