The Genus Amylomyces

rDNA ITS Sequence ofRhizopus oryzae: Its Application to Classification and Identification of Lactic Acid Producers

“…The type strain of Amylomyces rouxii (CBS 438.76) was obtained from Centraalbureau voor Schimmelcultures (CBS, Utrecht, the Netherlands). For the identiˆac-tion of Amylomyces rouxii, basal synthetic medium (SBM) 4) was used.…”

Comparison of Amylomyces rouxii and Rhizopus oryzae in Lactic Acid Fermentation of Potato Pulp

“…The suspension was diluted serially and spread on the potato dextrose agar. (Abe et al, 2003;Ellis et al, 1976), all of the six SDM strains were identified as Amylomyces rouxii. Strain CBS 438.76T was originally isolated from look-pang made in Thailand (Ellis et al, 1976).…”

“…(Abe et al, 2003;Ellis et al, 1976), all of the six SDM strains were identified as Amylomyces rouxii. Strain CBS 438.76T was originally isolated from look-pang made in Thailand (Ellis et al, 1976). Ragi and look-pang, types of sweet fermented glutinous rice, are used as starter cultures for traditional foods.…”

“…Recently, sequences of 18S-28S rRNA spacer regions were shown to be identical to lactic acid-accumulating strains of R. olyzae and Amy'lomyces rouxii but not to strains of R. olyzae that produce mallc and fumaric acids (Abe et a/., 2003). Amylomyces is a monotypic genus containing the single variable species A. rouxii, which is closely related to R. oryzae, as identifiable from the foamation of rhizoids, stolons, and black-pigmented sporangia (Ellis et a/., 1976). A distinct morphological characteristic that A, rouxii and R, olyzae share is the enormous number of chlamydospores produced in the aerial and substrate mycelium.…”

Molecular Characterization of the Relationships amongAmylomyces rouxii,Rhizopus oryzae, andRhizopus delemar

“…5) Taxonomic study of A. rouxii had been done by Ellis et al, fundamentally based on morphological aspects such as the production of enormous numbers of chlamydospores produced in the aerial and substrate mycelium, and the lack of rhizoids, stolons, and blackpigmented sporangia, compared with the relative species, Rhizopus oryzae. 6) In addition, some differences have been found in the utilization of carbon sources between A. rouxii and R. oryzae. Ellis 7) reported similarity of A. rouxii and R. oryzae revealed by DNA-DNA complementation, and proposed to re-classify A. rouxii as R. arrhizus var.…”