The genus Coprinellus (Basidiomycota; Agaricales) in Pakistan with the description of four new species

Abstract

Mushrooms with a thin-fleshed pileus that becomes plicate on opening, deliquescent lamellae and dark brown to blackish basidiospores are commonly called coprinoid mushrooms. The genus Coprinellus is one of the important lineages of coprinoid mushroom in the family Psathyrellaceae. Species-level taxonomy in Coprinellus is based mainly on the presence or absence and the structure of veil and cystidia on the pileus, of cystidia on the lamellae and on basidiospore morphology. In this study, four new species of Coprinellus (Co.campanulatus, Co.disseminatus-similis, Co.pakistanicus and Co.tenuis) are described from Pakistan. Species descriptions are based on morphological and molecular data. Phylogenetic analyses based on nuc rDNA ITS region show that the new species Co.campanulatus and Co.disseminatus-similis are clustered in a clade including members of section Micacei; Co.tenuis falls in a clade with members of section Domestici; and Co.pakistanicus recovered in a separate clade adjacent to other recently described clades of genus Coprinellus. Morpho-anatomical descriptions of the new species and comparison with closely allied taxa are provided. With this study, the number of known species of Coprinellus in Pakistan has reached eight.

Fungal diversity notes 1387–1511: taxonomic and phylogenetic contributions on genera and species of fungal taxa

“…Notes : Coprinellus , consists of approximately 85 described species, characterized by independent lineages in Psathyrellaceae (Redhead et al 2001 ; Walther et al 2005 ; Padamsee et al 2008 ; Vašutová et al 2008 ; Nagy et al 2011 , 2012 , 2013 ; Örstadius et al 2015 ; Hussain et al 2018 ). Mushrooms belonging to Coprienllus are common saprotrophs and are divided into three major sections on the basis of veil anatomy and the presence or absence of pileocystidia.…”

“…Notes : Closely related species, including Coprinellus disseminatisimilis Hussain mostly resemble C. punjabensis (Fig. 200 ) in having cylindrical to campanulate pileus, length of cylindrical stipe 20–40 μm, absence of annulus, ellipsoid to amygdamliform dark brownish basidiospores and hyaline cylindrical to clavate basidia (Hussain et al 2018 ). Coprinellus disseminatus also resemble with our taxon in having whitish grey cylindrical stipe, lamellulae 0–3, globose to subglobose veil elements up to 40 μm (Uljé 2005 ) .…”

“…However, C. punjabensis differs from C. disseminatisimilis in smaller size of white greyish pileus up to 12 mm diam., lamellulae 0–3, pileipellis irregular epithelium, veil elements hyaline globose to sub-globose up to 45 μm while C. disseminatisimilis has larger greyish-brown to greyish-yellowish-brown pileus (15–20 μm diam. ), lamellulae 0–2, pileipellis light brown to hyaline loosely euhymenoderm, veil elements greyish brown up to 40 μm (Hussian et al 2018 ). Coprinellus disseminates also differs from C. punjabensis in having larger pale-brown to yellow–brown, sometime almost white conical to convex pileus (up to 20 μm diam.…”

“…201 ). To provide the recommendation to justify our new species, we follow Hussain et al ( 2018 ). Comparison of 625 nucleotides of the ITS sequences between C. punjabensis (MT180990) and C. disseminatisimilis (MH753670) reveals 32 substitutions and 6 deletions in our taxon (6.08% nucleotide difference) while comparison of 643 nucleotides of ITS sequences between C. punjabensis (MT180990) and C. disseminates (JN159560) reveals 23 substitutions and 27 deletions in our taxon (7.77% nucleotide differences), which we believe should be sufficient to delineate our new species.…”

Changes in landscape ecology between nature reserve and palm oil plantation in West Java, Indonesia based on the observations of macrofungal population

“…Microchemical tests were carried out with MR, KOH 5%, lactophenol cotton blue (CB) 1%, and ammonium hydroxide (NH4OH) 10% for mycelium; and MR, CB 1% and NH4OH 10% for spores (Dwivedi et al 2012;Mohanan 2014). Macrofungi were identified from the standard literature and the description of legitimate current name status registered in Mycobank database (Gäumann 1928;Thomas 1928;Corner 1950;Cooke 1961;Denison 1963;Dennis 1978;Dutta et al 2014;Dwidjoseputro 1978;Dring 1980;Arora 1986;Gilbertson and Ryvarden 1986;Largent 1986;Singer 1986;Hjortstam et al 1987;Miller and Miller 1988;Largent et al 1997;Pegler et al 1997;Spooner 2000;Lodge 2004;Deacon 2005;Halling and Mueller 2005;Cannon and Kirk 2007;Parfitt et al 2007;Kirk et al 2008;Laessøe 2013;Petersen 2013;Beug et al 2014;Shay et al 2017;Wu et al 2017;Hussain et al 2018;Laessøe and Petersen 2019a, b).…”