The Genus Zymomonas

Sahm, Hermann; Bringer-Meyer, Stephanie; Sprenger, Georg A.

doi:10.1007/0-387-30745-1_10

Cited by 16 publications

(7 citation statements)

References 212 publications

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“…Zymomonas strains have been found in a variety of plant juices, mainly from tropical and subtropical regions, and they are perhaps the most important alcoholic fermenters of the bacterial group in plant saps and juices (Obire 2005). Zymomonas mobilis performs a highly productive ethanolic fermentation, producing an equimolar mixture of ethanol and CO 2 from more than 95% of the glucose utilized (Swings and De Ley 1977; Sahm et al. 2006).…”

Section: Discussionmentioning

confidence: 99%

The bacterial community in ‘taberna’ a traditional beverage of Southern Mexico

Alcántara-Hernández

Rodríguez-Álvarez

Valenzuela‐Encinas

et al. 2010

Letters in Applied Microbiology

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Aims: To characterize the bacterial community of taberna, an alcoholic traditional beverage from the Southern part of Mexico produced by the fermentation of the coyol palm sap (Acrocomia aculeate). Methods and Results: Bacterial 16S rDNA libraries were constructed from metagenomic DNA extracted during the fermentation process at 0, 60 and 108 h. A total of 154 clones were sequenced, and 13, 10 and nine unique sequences were found at each sampling time. At the onset of the fermentation, Zymomonas mobilis, Fructobacillus spp., Pantoea agglomerans and other Gammaproteobacteria were detected. After 60 h, lactic acid bacteria were found and 30% of clones in the library were related to Lactobacillus nagelii, L. sucicola and L. sp. By the end of the experiment, i.e. after 108 h, the bacterial community included Z. mobilis, Lact. nagelii and Acetobacter pasteurianus. Conclusions: Our results suggest that Z. mobilis population represented an important proportion of the bacterial community (60–80%), as well as the lactobacilli during the fermentation process. The bacterial diversity was low and decreased as the fermentation progressed. Significance and Impact of the Study: This culture‐independent study suggests that Z. mobilis and lactobacilli play an important role in the alcoholic fermentation of the taberna beverage.

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Section: Discussionmentioning

confidence: 99%

The bacterial community in ‘taberna’ a traditional beverage of Southern Mexico

Alcántara-Hernández

Rodríguez-Álvarez

Valenzuela‐Encinas

et al. 2010

Letters in Applied Microbiology

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“…Unfortunately, the genetic manipulation of Z. mobilis is still restricted since all known Z. mobilis strains are inherently resistant to a variety of antibiotics , most strains of Z. mobilis contain several indigenous plasmids , and no bacteriophage has been reported for Z. mobilis yet . More importantly, Z. mobilis has a strong host‐dependent restriction/modification system and rec A‐dependent repair system, causing it to be resistant to transformation and be highly resistant to mutagenesis . However, these characteristics have still not been fully explained from analysis of the genomic sequence.…”

Section: Introductionmentioning

confidence: 99%

Enhanced electrotransformation of the ethanologen Zymomonas mobilis ZM4 with plasmids

Zou

Zhang

You

et al. 2012

Engineering in Life Sciences

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Enhanced electrotransformation of the ethanologen Zymomonas mobilis ZM4 with plasmidsThe Zymomonas mobilis ZM4 strain with excellent ethanol-producing capabilities was the first strain of Z. mobilis, which was sequenced. This strain is resistant to transformation, and no previous study has shown a detailed protocol for electrotransfer of ZM4 with foreign DNA. In this work, many electrical and biological parameters were selected and evaluated in order to optimize the electrotransformation of ZM4. First, improved transformation efficiencies of 11 896, 99, 96 and 5989 transformants/mg DNA were separately achieved with shuttle plasmid pZB21-mini (3082 bp), pZB21 (5930 bp), pZA22 (6994 bp) and broad-host-range vector pBBR1MCS-2 (5144 bp) all prepared from Escherichia coli JM110. The crucial factors affecting the transformation efficiency included the source of the plasmid (the best strain was ZM4), origin and size of the plasmids, growth phase of the cells (the most ideal phase was early log phase with OD 600 of 0.3-0.4), the electric field strength (generally 11.75 kV/cm-13.25 kV/cm) and the recovery time (3-24 h). Further, based upon the optimal transformation protocol mentioned above for replicative plasmids in ZM4, (i) the electrotransformation by recombinant plasmid pBBR1MCS-2-Pgap-FLP (6880 bp) was an immediate success with the transformation efficiency 10 2 transformants/mg DNA; (ii) the site-specific integration efficiencies (expressed in terms of ''per mg of DNA'') of 3-6 integrating transformants was obtained using the integrating plasmid pBR328-ldhR-cml-ldhL (7447 bp). This study will assist genetic and biotechnological research of ZM4 and other Z. mobilis strains by providing information about suitable vectors and a more universal and reliable procedure for introducing DNA into this strain.

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“…Namun demikian mikroorganisme penghasil bioetanol lainnya, yaitu Zymomonas mobilis (Z. mobilis) juga menunjukkan potensi yang mirip dengan dua model bakteri sebelumnya. Z.mobilis mampu memanfaatkan substrat gula secara optimal untuk memproduksi etanol dengan hasil yang lebih tinggi, dengan tingkat (jumlah) pertumbuhan sel (biomassa) yang relatif rendah, sekaligus tanpa memerlukan kontrol oksigen selama masa fermentasi (Doelle et al, 1993;Seo et al, 2005, Lin andTanaka, 2006;Panesar et al, 2006;Sahm et al, 2006;Rogers et al, 2007). Bakteri ini juga sangat sesuai untuk pembuatan etanol berbahan lignoselulosa karena memiliki toleransi yang cukup tinggi terhadap berbagai macam inhibitor yang ada pada hidrolisat lignoselulosa (Shui et al, 2015).…”

Section: Pendahuluanunclassified