The Global Arginine Regulator ArgR Controls Expression of
 argF
 in
 Pseudomonas syringae
 pv. phaseolicola but Is Not Required for the Synthesis of Phaseolotoxin or for the Regulated Expression of
 argK

Hernández-Flores, José Luis; López‐López, Karina; Garcidueñas-Piña, Rogelio; Jofre-Garfías, A.E.; Alvarez‐Morales, Ariel

doi:10.1128/jb.186.11.3653-3655.2004

Cited by 7 publications

(7 citation statements)

References 28 publications

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“…In order to discard any activity corresponding to the phaseolotoxin-sensitive OCTase, we preincubated the reaction mixture with a phaseolotoxin containing supernatant; later, OCTase activity was determined as previously reported [24]. The results obtained are shown in Figure 2.…”

Section: Resultsmentioning

confidence: 99%

“…Based in the previous RT-PCR analyses showing an increase in argK expression at 28°C in mutant YNorf1P ( Figure 1B ) and considering that the argK gene codes for the phaseolotoxin-resistant OCTase, we analyzed the OCTase specific activity at 28°C in YNorf1P in comparison to that of the wild type strain NPS3121. In order to discard any activity corresponding to the phaseolotoxin-sensitive OCTase, we preincubated the reaction mixture with a phaseolotoxin containing supernatant; later, OCTase activity was determined as previously reported [24] . The results obtained are shown in Figure 2 .…”

Section: Resultsmentioning

confidence: 99%

“…The OCTase activity was determined by measuring OCTase specific activity as previously described [24] by using 5 ml cultures of strains NPS3121 (wild type) and Ynorf1P ( phtA − ) grown in M9 medium at 28°C during 24 h. Cells were disrupted with a VirTis sonicator (model VirSonic 60), and 3 µl of this crude extract were used for the assay. The reaction mixtures were incubated at 37°C for 20 min and OCTase activity was determined.…”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, the OCTase present under conditions nonpermissive for phaseolotoxin synthesis in P. syringae pv. phaseolicola, encoded by gene argF , is negatively regulated by ArgR [24] . However, production of ROCT and synthesis of phaseolotoxin occur independently of ArgR.…”

Section: Introductionmentioning

confidence: 99%

“…However, production of ROCT and synthesis of phaseolotoxin occur independently of ArgR. Therefore there is not any apparent metabolic link between the genes for phaseolotoxin synthesis/resistance and the genes involved in the primary metabolism [24] .…”

Section: Introductionmentioning

confidence: 99%

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Expression of the Gene for Resistance to Phaseolotoxin (argK) Depends on the Activity of Genes phtABC in Pseudomonas syringae pv. phaseolicola

et al. 2012

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The bacterium Pseudomonas syringae pv. phaseolicola produces phaseolotoxin in a temperature dependent manner, being optimally produced between 18°C and 20°C, while no detectable amounts are present above 28°C. Phaseolotoxin is an effective inhibitor of ornithine carbamoyltransferase (OCTase) activity from plant, mammalian and bacterial sources and causes a phenotypic requirement for arginine. To protect the cell from its own toxin, P. syringae pv. phaseolicola synthesizes a phaseolotoxin-resistant OCTase (ROCT). The ROCT is the product of the argK gene and is synthesized only under conditions leading to phaseolotoxin synthesis. The argK gene is included in a chromosomal fragment named Pht cluster, which contains genes involved in the synthesis of phaseolotoxin. The aim of the present work was to investigate the possible involvement of other genes included in the Pht cluster in the regulation of gene argK. We conducted transcriptional analyses of argK in several mutants unable to produce phaseolotoxin, transcriptional fusions and electrophoretic mobility shift assays, which allowed us to determine that genes phtABC, located within the Pht cluster, participate in the transcriptional repression of gene argK at temperatures not permissive for phaseolotoxin biosynthesis. This repression is mediated by a protein present in both toxigenic and nontoxigenic strains of P. syringae and in E. coli, and requires the coordinated participation of phtA, phtB and phtC products in order to carry out an efficient argK repression.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Expression of the Gene for Resistance to Phaseolotoxin (argK) Depends on the Activity of Genes phtABC in Pseudomonas syringae pv. phaseolicola

et al. 2012

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Current Ideas on the Genetics and Regulation of the Synthesis of Phaseolotoxin in Pseudomonas Syringae PV. Phaseolicola

Alvarez‐Morales¹,

López‐López²,

Hernández-Flores³

2004

Pseudomonas

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Comparative Analysis of argK-tox Clusters and Their Flanking Regions in Phaseolotoxin-Producing Pseudomonas syringae Pathovars

et al. 2006

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DNA fragments containing argK-tox clusters and their flanking regions were cloned from the chromosomes of Pseudomonas syringae pathovar (pv.) actinidiae strain KW-11 (ACT) and P. syringae pv. phaseolicola strain MAFF 302282 (PHA), and then their sequences were determined. Comparative analysis of these sequences and the sequences of P. syringae pv. tomato DC3000 (TOM) (Buell et al., Proc Natl Acad Sci USA 100:10181-10186, 2003) and pv. syringae B728a (SYR) (Feil et al., Proc Natl Acad Sci USA 102:11064-11069, 2005) revealed that the chromosomal backbone regions of ACT and TOM shared a high similarity to each other but presented a low similarity to those of PHA and SYR. Nevertheless, almost-identical DNA regions of about 38 kb were confirmed to be present on the chromosomes of both ACT and PHA, which we named "tox islands." The facts that the GC content of such tox islands was 6% lower than that of the chromosomal backbone regions of P. syringae, and that argK-tox clusters, which are considered to be of exogenous origin based on our previous studies (Sawada et al., J Mol Evol 54:437-457, 2002), were confirmed to be contained within the tox islands, suggested that the tox islands were an exogenous, mobile genetic element inserted into the chromosomes of P. syringae strains. It was also predicted that the tox islands integrated site-specifically into the homologous sites of the chromosomes of ACT and PHA in the same direction, respectively, wherein 34 common gene coding sequences (CDSs) existed. Furthermore, at the left end of the tox islands were three CDSs, which encoded polypeptides and had similarities to the members of the tyrosine recombinase family, suggesting that these putative site-specific recombinases were involved in the recent horizontal transfer of tox islands.

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The Global Arginine Regulator ArgR Controls Expression of argF in Pseudomonas syringae pv. phaseolicola but Is Not Required for the Synthesis of Phaseolotoxin or for the Regulated Expression of argK

Cited by 7 publications

References 28 publications

Expression of the Gene for Resistance to Phaseolotoxin (argK) Depends on the Activity of Genes phtABC in Pseudomonas syringae pv. phaseolicola

Expression of the Gene for Resistance to Phaseolotoxin (argK) Depends on the Activity of Genes phtABC in Pseudomonas syringae pv. phaseolicola

Current Ideas on the Genetics and Regulation of the Synthesis of Phaseolotoxin in Pseudomonas Syringae PV. Phaseolicola

Comparative Analysis of argK-tox Clusters and Their Flanking Regions in Phaseolotoxin-Producing Pseudomonas syringae Pathovars

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