The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by
            <i>Pseudomonas aeruginosa</i>

O’Toole, George A.; Gibbs, Karine A.; Hager, Paul W.; Phibbs, Paul V.; Kolter, Roberto

doi:10.1128/jb.182.2.425-431.2000

Cited by 282 publications

(251 citation statements)

References 36 publications

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“…Previous studies showed that the formation of mushroom-like structures by P. aeruginosa biofilms depended on the carbon and energy source provided in the growth medium (39,40). For biofilms grown in minimal medium, glucose supported formation of mushroom-like structures, but citrate did not.…”

Section: Discussionmentioning

confidence: 98%

Iron and Pseudomonas aeruginosa biofilm formation

Banin

Vasil

Greenberg

2005

Proc. Natl. Acad. Sci. U.S.A.

727

643

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Iron serves as a signal in Pseudomonas aeruginosa biofilm development. We examined the influence of mutations in known and putative iron acquisition-signaling genes on biofilm morphology. In iron-sufficient medium, mutants that cannot obtain iron through the high-affinity pyoverdine iron acquisition system form thin biofilms similar to those formed by the parent under low iron conditions. If an iron source for a different iron acquisition system is provided to a pyoverdine mutant, normal biofilm development occurs. This enabled us to identify iron uptake gene clusters that likely serve in transport of ferric citrate and ferrioxamine. We suggest that the functional iron signal for P. aeruginosa biofilm development is active transport of chelated iron or the level of internal iron. If the signal is internal iron levels, then a factor likely to be involved in iron signaling is the cytoplasmic ferric uptake regulator protein, Fur, which controls expression of iron-responsive genes. In support of a Fur involvement, we found that with low iron a Fur mutant was able to organize into more mature biofilms than was the parent. The two known Fur-controlled small regulatory RNAs (PrrF1 and F2) do not appear to mediate iron control of biofilm development. This information establishes a mechanistic basis for iron control of P. aeruginosa biofilm formation.pyoverdine

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Section: Discussionmentioning

confidence: 98%

Iron and Pseudomonas aeruginosa biofilm formation

Banin

Vasil

Greenberg

2005

Proc. Natl. Acad. Sci. U.S.A.

727

643

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“…This absorptive layer, referred to as a conditioning film (reviewed in reference 39), not only changes the physicochemical properties of the surface but also contributes to the accumulation of proteins, polysaccharides, and other molecules at the surface that provide a metabolically favorable environment for bacterial cells and serve as nutritional cues to trigger biofilm formation. In Pseudomonas aeruginosa, these cues are integrated by the catabolite repression control protein, which plays a role in the regulation of carbon metabolism and biofilm formation, as a crc mutant formed only a dispersed monolayer of cells devoid of microcolonies which are typical of mature biofilms of the wild-type strain (123). One would therefore assume that an abundant supply of nutrients fosters attachment and biofilm formation.…”

Section: Why Attach In the First Place?mentioning

confidence: 99%

Sticky Situations: Key Components That Control Bacterial Surface Attachment

Petrova

Sauer

2012

Journal of Bacteriology

323

251

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“…One important characteristic of biofilms is their high resistance to antibiotics, which makes the treatment of biofilm-related infections difficult. Several surface-related environmental signals, as well as surface-contact mediated by specific cell surface structures, are recognized to trigger changes that allow bacteria to undergo stable cell-surface interactions (2)(3)(4)(5). However, it remains a challenge to identify regulatory processes critical for adhesion, the understanding of which will be useful to devise better treatments to combat biofilm formation.…”

mentioning

confidence: 99%

Surface sensing and adhesion of Escherichia coli controlled by the Cpx-signaling pathway

Otto

Silhavy

2002

Proc. Natl. Acad. Sci. U.S.A.

370

343

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Bacterial adhesion is an important initial step in biofilm formation, which may cause problems in medical, environmental, and industrial settings. In spite of obvious phenotypic differences between attached and planktonic cells, knowledge about the genetic basis for these differences and how adhesion-induced changes are mediated is limited. The Cpx two-component signal transduction pathway responds specifically to stress caused by disturbances in the cell envelope and activates genes encoding periplasmic protein folding and degrading factors. Here, we address the role of the Cpx-signaling pathway in sensing and responding to the physical change occurring during adhesion of Escherichia coli to surfaces. We present evidence that the expression of Cpx-regulated genes is induced during initial adhesion of E. coli to abiotic surfaces. This induction is specifically observed upon attachment of stationaryphase cells to hydrophobic surfaces. Moreover, surface-induced activity of the Cpx response requires NlpE, an outer membrane lipoprotein, which has previously been shown to induce the Cpx system when overproduced. The importance of a functional Cpx response during adhesion is further supported by the fact that a dramatically lower number of cells attach to the surface and dynamic cell-surface interactions as measured by a quartz crystal microbalance technique are altered when the CpxRA pathway is disrupted. The defects in adhesion exhibited by the cpxR and nlpE mutants were strikingly similar to those of wild-type cells in which protein synthesis was inhibited, suggesting that the Cpx pathway plays a key role in the regulation of adhesion-induced gene expression.biofilm formation ͉ cell envelope stress ͉ NlpE ͉ signal transduction ͉ quartz crystal microbalance B acteria often survive within biofilms, which are implicated in environmental problems and numerous infections (1). One important characteristic of biofilms is their high resistance to antibiotics, which makes the treatment of biofilm-related infections difficult. Several surface-related environmental signals, as well as surface-contact mediated by specific cell surface structures, are recognized to trigger changes that allow bacteria to undergo stable cell-surface interactions (2-5). However, it remains a challenge to identify regulatory processes critical for adhesion, the understanding of which will be useful to devise better treatments to combat biofilm formation.As the interface with the external environment, the bacterial cell envelope is directly exposed to changes in environmental conditions and is particularly sensitive to any perturbations. In Escherichia coli, stressful conditions affecting the cell envelope are perceived by two regulatory pathways, the E regulatory system and the CpxRA two-component signal transduction pathway (6). Although both pathways similarly respond to general stress conditions in the extracytoplasmic compartment of the cell and partially overlap in their regulons, they can be distinguished by their response to specific signals...

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The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa

Cited by 282 publications

References 36 publications

Iron and Pseudomonas aeruginosa biofilm formation

Iron and Pseudomonas aeruginosa biofilm formation

Sticky Situations: Key Components That Control Bacterial Surface Attachment

Surface sensing and adhesion of Escherichia coli controlled by the Cpx-signaling pathway

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