1981
DOI: 10.1016/0014-5793(81)80116-0
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The glutamate dehydrogenase system and the redox state of mitochondrial free nicotinamide adenine dinucleotide in myocardium

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Cited by 36 publications
(14 citation statements)
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“…According to the above data the equilibrium of the LDH reaction in the cytosol of the cardiac myocyte cannot be attested by optical methods as was possible in the case of GDH in the mitochondria in situ (5). The equilibria were therefore tested by also measuring the concentration ratio between glycerol-3-phosphate and dihydroxyacetone phosphate in the tissue (table I).…”
Section: Indicator Metabolite Systemsmentioning
confidence: 99%
See 1 more Smart Citation
“…According to the above data the equilibrium of the LDH reaction in the cytosol of the cardiac myocyte cannot be attested by optical methods as was possible in the case of GDH in the mitochondria in situ (5). The equilibria were therefore tested by also measuring the concentration ratio between glycerol-3-phosphate and dihydroxyacetone phosphate in the tissue (table I).…”
Section: Indicator Metabolite Systemsmentioning
confidence: 99%
“…Information on the cytosolic and mitochondrial redox states of the NAD § couple is much more difficult to obtain in heart muscle. Only the glutamate dehydrogenase system (GDH) in the mitochondria appears to be amenable to the indicator metabolite method (5). Tissue L/P and glycerol-3-phosphate/dihydroxyacetone phosphate ratios have been correlated with changes of the NADH fluorescence in perfused rat heart during transition to anoxia (6), but systematic evaluation of the cytosolic redox indicator reactions in myocardium is lacking.…”
Section: Introductionmentioning
confidence: 99%
“…The rat heart mitochondria were isolated using standard methods (20). 5 Autofluorescence emission spectrum of isolated rat heart mitochondria collected using the same apparatus as shown in Fig. 1.…”
Section: Cell Viability and Spectral Characteristicsmentioning
confidence: 99%
“…Cellular NADH (reduced) and NAD+ (oxidized) levels have been measured using enzymatic assays on tissue extracts. The determination of the NADH and NAD+ compartmentation has relied on the measurement of specific redox couples participating in localized enzymatic reactions, such as the lactate dehydrogenase system in the cytosol (4) and the glutamate dehydrogenase system in the mitochondrial matrix (5). Surface fluorescence has been used to measure the relative level of NADH in both perfused (3) and in vivo tissues (6).…”
Section: Introductionmentioning
confidence: 99%
“…Experimental model was the isolated normoxic guinea-pig heart [19,201 perfused with glucose as the sole substrate to support net glycolytic flux and simultaneously establish lactate dehydrogenase near-equilibrium [3,4,6,71. Glycolytic rate was minimized by employing hyperkalemic mechanical arrest and stimulated by normal and maximum norepinephrine doses using the non-working Langendorff heart.…”
mentioning
confidence: 99%