The glyceraldehyde-3-phosphate dehydrogenase of the shrimp Litopenaeus vannamei : Molecular cloning, characterization and expression during hypoxia

Camacho‐Jiménez, Laura; Peregrino‐Uriarte, Alma B.; Martínez-Quintana, José A.; Yépiz-Plascencia, Glória

doi:10.1016/j.marenvres.2018.04.003

Cited by 28 publications

(15 citation statements)

References 49 publications

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“…Comparable to a previous study (Camacho-Jiménez et al, 2018), hypoxia strongly induced GAPDH expression in gills at 3 h, a response that was completely blunted in HIF-1 silenced animals. This suggests that, similar to mammalian cells (Higashimura et al, 2011;Lu et al, 2002), GAPDH expression is induced via HIF-1 by binding to HREs upon oxygen deficiency.…”

Section: Discussionsupporting

confidence: 45%

“…Furthermore, although GAPDH is a key glycolytic enzyme, studies from prokaryotes to vertebrates have shown that it has multiple roles unrelated to its enzyme activity in glycolysis in the cells, and thus, it is considered a "moonlighting protein" (White and Garcin, 2017). In crustaceans, GAPDH gene expression and protein activity are sensitive to several stressors such as diseases (Liu et al, 2011;Wang et al, 2007), high hydrostatic pressure (Morris et al, 2015), hydrocarbons (Pasquevich et al, 2013), water acidification (Chang et al, 2016;Hauton et al, 2009), cold stress (Fan et al, 2013), hypercapnic hypoxia (Johnson et al, 2015) and hypoxia (Camacho-Jiménez et al, 2018). Although most of the knowledge on GAPDH molecular biology in animal models can be translated from the findings in vertebrates, the specific mechanisms underlying the transcriptional control of GAPDH are poorly understood in crustaceans as well as in most aquatic invertebrates.…”

Section: Discussionmentioning

confidence: 99%

“…The integrity of total RNA was validated by 1% agarose gel electrophoresis. Contaminating gDNA was eliminated from total RNA by digestion with RNAse-free DNAse I, (Roche, Mannheim, Germany) and was verified by qPCR using the specific primers for the GAPDH gene GAPDHFw2/GAPDHRv1 (Table 1) that generate a product of 143 bp (Camacho-Jiménez et al, 2018). Each qPCR reaction (15 μL) contained: 7.5 μL of 2× SyBr green qPCR Master Mix (Biotool, Houston, TX, USA), 25 ng total RNA, and 0.5 μM each primer.…”

Section: Mrna Quantification By Rt-qpcrmentioning

confidence: 99%

“…Recently, the GAPDH gene from L. vannamei was cloned and sequenced, and its expression in gills was shown to be up-regulated during hypoxia (Camacho-Jiménez et al, 2018). Gills are involved in respiration, and energy-requiring processes like osmoregulation, detoxification and acid-base balance (Henry et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Regulation of glyceraldehyde-3-phosphate dehydrogenase by hypoxia inducible factor 1 in the white shrimp Litopenaeus vannamei during hypoxia and reoxygenation

Camacho‐Jiménez

Leyva‐Carrillo

Peregrino‐Uriarte

et al. 2019

Comparative Biochemistry and Physiology Part A: Molecular &

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Hypoxia is a frequent source of stress in the estuarine habitat of the white shrimp Litopenaeus vannamei. During hypoxia, L. vannamei gill cells rely more heavily on anaerobic glycolysis to obtain ATP. This is mediated by transcriptional up-regulation of glycolytic enzymes including glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The hypoxia inducible factor 1 (HIF-1) is an important transcriptional activator of several glycolytic enzymes during hypoxia in diverse animals, including crustaceans. In this work, we cloned and sequenced a fragment corresponding to the 5′ flank of the GAPDH gene and identified a putative HIF-1 binding site, as well as sites for other transcription factors involved in the hypoxia signaling pathway. To investigate the role of HIF-1 in GAPDH regulation, we simultaneously injected double-stranded RNA (dsRNA) into shrimp to silence HIF-1α and HIF-1β under normoxia, hypoxia, and hypoxia followed by reoxygenation, and then measured gill HIF-1α, HIF-1β expression, GAPDH expression and activity, and glucose and lactate concentrations at 0, 3, 24 and 48 h. During normoxia, HIF-1 silencing induced up-regulation of GAPDH transcripts and activity, suggesting that expression is down-regulated via HIF-1 under these conditions. In contrast, HIF-1 silencing during hypoxia abolished the increases in GAPDH expression and activity, glucose and lactate concentrations. Finally, HIF-1 silencing during hypoxia-reoxygenation prevented the increase in GAPDH expression, however, those changes were not reflected in GAPDH activity and lactate accumulation. Altogether, these results indicate that GAPDH and glycolysis are transcriptionally regulated by HIF-1 in gills of white shrimp.

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Section: Discussionsupporting

confidence: 45%

Section: Discussionmentioning

confidence: 99%

Section: Mrna Quantification By Rt-qpcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regulation of glyceraldehyde-3-phosphate dehydrogenase by hypoxia inducible factor 1 in the white shrimp Litopenaeus vannamei during hypoxia and reoxygenation

Camacho‐Jiménez

Leyva‐Carrillo

Peregrino‐Uriarte

et al. 2019

Comparative Biochemistry and Physiology Part A: Molecular &

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“…Glycolysis is the main pathway of anaerobic metabolism [36]. Glyceraldehyde-3-phosphate dehydrogenase (GAPD H) is a multifunctional enzyme in glycolysis [37,38]. It can catalyze the mutual conversion of 1,3-diphosphoglyceric acid and 3-phosphoglycerate and it participates in multiple biological processes, including DNA repair, membrane fusion and transport, RNA binding, autophagy, cell death cytoskeleton dynamics, and cytoskeleton dynamics [39].…”

Section: Discussionmentioning

confidence: 99%

Multi-omics analysis reveals the glycolipid metabolism response mechanism in the liver of genetically improved farmed Tilapia (GIFT, Oreochromis niloticus) under hypoxia stress

Qiang

Tao

et al. 2021

BMC Genomics

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Background Dissolved oxygen (DO) in the water is a vital abiotic factor in aquatic animal farming. A hypoxic environment affects the growth, metabolism, and immune system of fish. Glycolipid metabolism is a vital energy pathway under acute hypoxic stress, and it plays a significant role in the adaptation of fish to stressful environments. In this study, we used multi-omics integrative analyses to explore the mechanisms of hypoxia adaptation in Genetically Improved Farmed Tilapia (GIFT, Oreochromis niloticus). Results The 96 h median lethal hypoxia (96 h-LH50) for GIFT was determined by linear interpolation. We established control (DO: 5.00 mg/L) groups (CG) and hypoxic stress (96 h-LH50: 0.55 mg/L) groups (HG) and extracted liver tissues for high-throughput transcriptome and metabolome sequencing. A total of 581 differentially expressed (DE) genes and 93 DE metabolites were detected between the CG and the HG. Combined analyses of the transcriptome and metabolome revealed that glycolysis/gluconeogenesis and the insulin signaling pathway were down-regulated, the pentose phosphate pathway was activated, and the biosynthesis of unsaturated fatty acids and fatty acid metabolism were up-regulated in GIFT under hypoxia stress. Conclusions The results show that lipid metabolism became the primary pathway in GIFT under acute hypoxia stress. Our findings reveal the changes in metabolites and gene expression that occur under hypoxia stress, and shed light on the regulatory pathways that function under such conditions. Ultimately, this information will be useful to devise strategies to decrease the damage caused by hypoxia stress in farmed fish.

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Temperate Versus Arctic: Unraveling the Effects of Temperature on Oil Toxicity in Gammarids

van den Heuvel‐Greve,

Jonker,

Klaassen

et al. 2024

Enviro Toxic and Chemistry

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Shipping activities are increasing with sea ice receding in the Arctic, leading to higher risks of accidents and oil spills. Because Arctic toxicity data are limited, oil spill risk assessments for the Arctic are challenging to conduct. In the present study, we tested if acute oil toxicity metrics obtained at temperate conditions reflect those at Arctic conditions. The effects of temperature (4 °C, 12 °C, and 20 °C) on the median lethal concentration (LC50) and the critical body residue (CBR) of the temperate invertebrate Gammarus locusta exposed to water accommodated fractions of a fuel oil were determined. Both toxicity metrics decreased with increasing temperature. In addition, data for the temperate G. locusta were compared to data obtained for Arctic Gammarus species at 4 °C. The LC50 for the Arctic Gammarus sp. was a factor of 3 higher than that for the temperate G. locusta at 4 °C, but its CBR was similar, although both the exposure time and concentration were extended to reach lethality. Probably, this was a result of the larger size and higher weight and total lipid content of Arctic gammarids compared to the temperate gammarids. Taken together, the present data support the use of temperate acute oil toxicity data as a basis for assessing risks in the Arctic region, provided that the effects of temperature on oil fate and functional traits (e.g., body size and lipid content) of test species are considered. As such, using the CBR as a toxicity metric is beneficial because it is independent of functional traits, despite its temperature dependency. To the best of our knowledge, the present study is the first to report CBRs for oil. Environ Toxicol Chem 2024;00:1–11. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

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The glyceraldehyde-3-phosphate dehydrogenase of the shrimp Litopenaeus vannamei : Molecular cloning, characterization and expression during hypoxia

Cited by 28 publications

References 49 publications

Regulation of glyceraldehyde-3-phosphate dehydrogenase by hypoxia inducible factor 1 in the white shrimp Litopenaeus vannamei during hypoxia and reoxygenation

Regulation of glyceraldehyde-3-phosphate dehydrogenase by hypoxia inducible factor 1 in the white shrimp Litopenaeus vannamei during hypoxia and reoxygenation

Multi-omics analysis reveals the glycolipid metabolism response mechanism in the liver of genetically improved farmed Tilapia (GIFT, Oreochromis niloticus) under hypoxia stress

Temperate Versus Arctic: Unraveling the Effects of Temperature on Oil Toxicity in Gammarids

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