The Glycine-Rich Domain Protein GRDP2 Regulates Ovule Development via the Auxin Pathway in Arabidopsis

Abstract

The glycine-rich domain proteins (GRDP) have been functionally implicated in the cell wall structure, biotic, and abiotic stress responses. However, little is known about GRDP genes in female gametophyte development of Arabidopsis. This study shows that GRDP2, a GRDP, plays a crucial role in female gametophyte development. In GRDP2 overexpression lines, grdp2-3, the embryo sacs were arrested at FG1 and no nucleus stages. Furthermore, callose staining shows that cell plate formation during megasporogenesis is disturbed in grdp2-3. In contrast, the pollen development is not affected in grdp2-3. The expression patterns of auxin-specific marker lines in female gametophytes showed that the auxin distribution and transport were significantly changed during megagametogenesis in grdp2-3. In addition, compared with the membrane-localized pattern of PIN1, PIN2, and PIN7 in WT, the signals were detected in the cytoplasm in grdp2-3. Together, our data suggest that GRDP2 plays an essential role in auxin-mediated female gametophyte development.

Using landscape genomics to assess local adaptation and genomic vulnerability of a perennial herb Tetrastigma hemsleyanum (Vitaceae) in subtropical China

“…It is noteworthy that several genes involved in flora reproductive development and flowering time were also identified ( Table 3 ). GRDP2 encodes a novel glycine-rich domain protein that modulates flowering time and ovule development ( Ortega-Amaro et al, 2015 ; Czolpinska and Rurek, 2018 ; Wang et al, 2021b ), and was previously identified to be involved in environmental response in Arabidopsis ( Mangeon et al, 2010 ; Ortega-Amaro et al, 2015 ). BAM1 has been shown to regulate anther development in Arabidopsis ( Hord et al, 2006 ), and MS5 is known to be essential for male fertility in Arabidopsis and Brassica species ( Glover et al, 1998 ; Zeng et al, 2021 ).…”

The Maize ZmBES1/BZR1-9 Transcription Factor Accelerates Flowering in Transgenic Arabidopsis and Rice

“…Likewise, PPC1 exhibits a circadian-regulated expression pattern, is involved in light-regulated development via interaction with the COP9 signalosome subunit 5, and regulates the flowering transition in the photoperiod-dependent pathway [ 52 , 53 , 54 ]. AtGRDP2 also regulates female gametophyte development via the auxin pathway, and plants overexpressing it show early flowering [ 50 , 55 ]. Meanwhile, BES1 directly represses AtMYB56 , which positively regulates the quiescent center and cell division [ 56 , 57 ].…”